Bioassay-Guided Isolation and Characterization of the Antibacterial Compound from Sonneratia apetala Buch. Ham Leaves Collected from the Maharashtra Coast of India

  • Jaimini Sarkar* Department of Life Sciences, University of Mumbai, Mumbai-98, India
  • Chiradeep Sarkar Department of Biotechnology, G.N. Khalsa College (Autonomous), Matunga, Mumbai-19, India
  • Bhaskar Laxman Jadhav Department of Life Sciences, University of Mumbai, Mumbai-98, India
Keywords: Phytochemicals, Antibacterial agents, Phytotherapy, Plant extracts, Ethnobotany


Background and Aim: Sonneratia apetala plant has been used in traditional medicine in south Asian countries for treating diarrhea, hepatitis, inflammation, wounds and ulcers. The leaf extract of this plant has shown promising antibacterial activity and it is essential to isolate that antibacterial compound in pure form. Materials and Methods: In this study, a bioassay-guided isolation and characterization approach was taken to isolate and characterize the antibacterial compound from the S. apetala Buch. Ham leaf. Petroleum ether, acetone and water extracts were prepared and tested against eight human pathogenic bacteria. Water and acetone extracts have shown the inhibition of bacteria but the more promising acetone extract was carried forward for further study. The compound was isolated using the preparative column chromatography method. The isolated compound was studied for antibacterial activity using TLC-bioautography. Further characterization was done using the UV-Vis spectroscopy and FT-IR spectroscopy. Results: The findings of the present study showed the presence of various valuable phytochemical constituents. Conclusion: The presence of an antibacterial compound highlights the importance of this plant as a source of phytochemicals with medicinal properties.  This study indicated that the geographical location of the plant is one of the factors which determines the antibacterial potency of the leaf extract.


Gore-Langton GR, Cano J, Simpson H, Tatem A, Garavito NT, Wigley A et al. Global estimates of pregnancies at risk of Plasmodium falciparum and Plasmodium vivax infection in 2020 and changes in risk patterns since 2000. PLOS Glob Public Health. 2022;2(11).

Agarwal SK, Srivastava RK. Chronic kidney disease in India: challenges and solutions. Nephron Clin Pract. 2009;111(3):c197-203.

Bahirwani R, Griffin C. The diagnosis and management of nonalcoholic fatty liver disease: A patient-friendly summary of the 2018 AASLD guidelines. Clin Liver Dis (Hoboken). 2022;19(6):222-6.

Teede HJ, Misso ML, Costello MF, Dokras A, Laven J, Moran L et al. Recommendations from the international evidence-based guideline for the assessment and management of polycystic ovary syndrome. Fertil Steril. 2018;110(3):364-79.

Udwadia ZF, Amale RA, Ajbani KK, Rodrigues C. Totally drug-resistant tuberculosis in India. Clin Infect Dis. 2011;54:67-71.

Garcia M, Jemal A, Ward EM, Center MM, Hao Y, Siegel RL et al. Global cancer facts and figures.; 2007. American Cancer Society.

Donnez J, Dolmans MM. Uterine fibroid management: from the present to the future. Hum Reprod Update. 2016;22(6):665-86.

Gibson AD, Yale G, Corfmat J, et al. Elimination of human rabies in Goa, India through an integrated One Health approach. Nat Commun. 2022;13(1):2788.

Ventola CL. The antibiotic resistance crisis: part 1: causes and threats. Pharm Ther. 2015;40(4):277-83.

Gajdács M. The concept of an ideal antibiotic: implications for drug design. Molecules. 2019;24(5):892.

Jaimini D, Jadhav BL. Wheat grass medicine to all. J Univ Mumbai. 2004;57:66-74.

Mukharjee D, Lokwani S, Dave A, Gonsalves C, Sarkar C. Bhringraj: A pharmaceutical treasure trove. Indian J Nat Sci. 2021;12(68):35231-35239.

Boukhatem MN, Setzer WN. Aromatic Herbs, Medicinal Plant-Derived Essential Oils, and Phytochemical Extracts as Potential Therapies for Coronaviruses: Future Perspectives. Plants.2020; 9(6):800.

Jaimini D, Jadhav BL. Studies on the protein extraction of Mango (Mangifera indica) seed endosperm. Trends Life Sci. 2004;19(1):1-8.

Jaimini D, Jadhav BL. Qualitative and quantitative analysis of endosperm proteins of Mangifera indica (Mango) Var. Totapuri. Trends Life Sci. 2005;20(1):39-47.

Jaimini D, Jadhav BL. Diet formulation for rohu (Labeo rohita) using Mango seed endosperm along with groundnut oil cake and fish meal. J Univ Mumbai. 2008; 58:9-18.

Morales G, Paredes A, Sierra P, Loyola LA. Antimicrobial activity of three Baccharis species used in the traditional medicine of Northern Chile. Molecules. 2008;13(4):790-4.

Parihar R, Akleshwar M. Role of phytochemicals in the field of chemical engineering. Int J Eng Res Technol. 2014;2(3):39-42.

Prabhakaran J, Kavitha D. Ethnomedicinal importance of Mangrove species of Pitchavaram. Int J Res Pharm Biomed Sci. 2012;3:611-614.

Glasenapp Y, Korth I, Nguyen X-V, Papenbrock J. Sustainable use of mangroves as sources of valuable medicinal compounds: species identification, propagation and secondary metabolite composition. S Afr J Bot. 2019;121:317-28.

Kathiresan K, Bingham BL. Biology of mangroves and mangrove ecosystems. Adv Mar Biol. 2001; 40:81-251.

Chen Y, Liao B, Peng Y, Xu S, Zheng S, Chen D. Researches on the northern introduction of mangrove species Sonneratla apetala Buch. Ham. Guangdong Forest. 2003; 19:9-12.

Mahmood H. Handbook of Selected Plant and Species of the Sundarbans and the Embankment Ecosystem. Sustainable Development and Biobdiversity Conservation in Coastal Protection Forests, Bangladesh (SDBC-Sundarbans) Project Implemented by the DEeutsche Gesellschaft Fur Internationale Zusammenarbeit (GIZ) GmbH on behalf of the German Federal Ministry for Economic Cooperation and Development (BMZ). Dhaka; 2015:116.

Hossain SJ, Basar MH, Rokeya B. Arif KMT, sultana M S, and Rahman M H 2013. Evaluation of Antioxidant, Antidiabetic and Antibacterial Activities of the Fruit of S. apetala (Buch. -Ham.). Oriental Pharmacy Experimental Medicine. 3:95-102.

Jayatissa LP, Dahdouh-Guebas F, Koedam N. A review of the floral composition and distribution of mangroves in Sri Lanka. Bot J Linn Soc. 2002;138(1):29-43.

Avijit H, Bhattacharyya D, Hazra AK, Sur TK. Anti-radical and anti-ulcer effect of S. apetala Buch. -Ham. leaves against alcohol induced gastric mucosal injury. Ars Pharm. 2021;62(4):348-57.

Bandaranayake WM. Traditional and medicinal uses of mangroves. Mangroves Salt Marshes. 1998;2(3):133-48.

Bandaranayake WM. Survey of mangrove plants from Northern Australia for phytochemical assessment and UV-absorbing compounds. Curr Top Phytochem. 1995;14:69-78.

Jaimini D, Sarkar C, Shabnam AA, Jadhav BL. Evaluation of antibacterial properties of mangrove plant S. apetala Buch. Ham. leaf. World Appl Sci J. 2011;14(11):1683-6.

Kumar PJ, Kumar Das S, Thatoi H. Phytochemical profiling and bioactivity of a mangrove plant, S. apetala, from Odisha coast of India. Chin J Integr Med. 2015;21(4):274-85.

Arifur Rahman Md, Rus AA’d. Md. Enamul Haque. Bangladesh J Microbiol. Isolation and characterization of antimicrobial compound from stem bark of traditional medicinal plant S. apetala: evaluation of their antimicrobial, cytotoxic and antioxidant properties. 2021;38(1):1-5.

Jana H. Mondol KC, Pati BR, Mitra A. Evaluation of anti-infective potential of fruits of common mangrove tree S. apetala against some selected pathogenic fungi and bacteria. Int J Herb Med. 2015;3(2):34-37.

Sunita S, Joshi M. Pharmacognostic evaluation of medicinally important mangroves Avicennia marina and S. apetala. World J Pharm Sci. 2015;3(7):1403-12.

Ji QF, Lin WH, Li J, et al. Chemical investigation of Chinese mangrove S. apetala. China J Chin Mater Med. 2005;30(16):1258-60.

Neelesh M, Malviya S. Bioassay guided fractionation-an emerging technique influence the isolation, identification and characterisation of lead phytomolecules. Int J Hosp Pharm. 2017;2(5):1-6.

Sattler K, Feidt HJ. Extraction. In: Thermal Separation Processes. VCH; 1995.

Spooner DF, Skyes G. Laboratory assessment of antimicrobial activity. In: Norris JR, Ribbons D, eds.. W. Publ: Academic Press London. Methods of Microbiology; vol 7(B); 1972:217-224.

Ecolink. Why is acetone a good solvent? www.; 2019.

Peifer JJ. A rapid and simplified method of analysis by thin layer chromatography using microchromatoplates. Microchim Acta. 1962;50(3):529-540.

Fisher R, Lautner H. Arch. Pharm. 1961 (Weinheim);294:1.

Nicolaus BJR, Coronelli C, Binaghi A. Farmaco (Pavia) Prat, ed.; 1961.

Ullah N, Khurram M, Amin MU, Khan TA, Khayyam SU, Khan FA. Umberin Najeeb and Saleem Ullah. Impact of geographical locations on Mentha spicata antibacterial activities. J Med Plants Res. 2012;6(7):1201-6.

Joshi B, Bhandari NL, Shrestha S, Shyaula SL, Gyawali R, Thapa P. Geographical distribution effect on phytoconstituents variation of Nepalese Zanthoxylum armatum Fruit extract and their antimicrobial properties. Advance Journal of Chemistry – B. 2021;3(3):295-310.

Seidel V, Peyfoon E, Watson DG, Fearnley J. Comparative study of the antibacterial activity of propolis from different geographical and climatic zones. Phytother Res. 2008;22(9):1256-1263. doi:10.1002/ptr.2480.

Banerjee D, Chakraborti S, Hazra AK, Banerjee S, Ray J, Mukherjee B. Antioxidant activity and total phenolics of some mangroves in Sundarbans. Afr J Biotechnol. 2008;7(6):805-810.

Elma SN, Badarusham K, Dzulfaldi R, Salvamani S, Hasan MS, Hashim R. Solvents extraction effects on bioactive compounds of Ajwa date (Phoenix dactylifera L.) flesh using mixture design. Chem Eng Trans. 2017;63:817-22.

Pavia DL, Gary ML, George SK, James RV. Introduction to Spectroscopy. 4th ed. Brooks/Cole; 2008.

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