The Antileishmanial Activity of Essential Oils from Some Traditionally Used Medicinal Plants in Iran

Hossein Mahmoudvand, Behrouz Ezatpour, Sareh Jahanbakhsh*

Abstract


Background and Aim: Cutaneous leishmaniasis (CL) is the most common type of leishmaniasis affecting 1.5 million people through the world annually. Treatment of CL by pentavalent antimony compounds remains a challenge because of adverse side effects. The present study was designed to evaluate the in vitro antileishmanial properties of six essential oils from selected spices and herbs ethno-medicinally used in Iran against Leishmania tropica and L. major promastigotes.

Materials and Methods: The in vitro antileishmanial effects of selected medicinal plants against L. tropica and L. major promastigotes were evaluated by colorimetric cell viability (MTT) assay. The IC50 values were also calculated by probit test using SPSS software.

Results: The findings demonstrated that all the tested essential oils had inhibitory effects on promastigote growth of L. tropica with IC50 values ranging from 3.2µg/mL to 19.3µg/mL and 2.7µg/mL to 18.8µg/mL for L. tropica and L. major, respectively. Zataria multiflora Boiss essential oil significantly (P<0.05) was much more effective than essential oils of the other tested plants and control drugs once they demonstrated lower IC50 values promastigote form.

Conclusions: The findings of present study indicated antileishmanial effects of some Iranian medicinal plants particularly Z. multiflora Boiss. However, further studies, on the animal models as well as volunteer human, are needed to confirm these results.


Keywords


Promastigote; Leishmania tropica; Leishmania major; Medicinal plants; cutaneous leishmaniasis

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References


World Health Organization. Control of the Leishmaniasis. Geneva: WHO (Technical Report Series 949). 2010;5–12.

Desjeux P. Leishmaniasis: current situation and new perspectives. Comp Immunol Microbiol Infect Dis. 2004;27:305–18.

Mahmoudvand H, Sharififar F, Sharifi I, Ezatpour B, Fasihi Harandi M, Makki MS, et al. In vitro inhibitory effect of Berberisvulgaris (Berberidaceae) and its main component, berberine against different Leishmania species. Iranian J Parasitol.2014;9(1):28-36.

Croft SL, Sundar S, Fairlamb AH. Drug resistance in leishmaniasis. Clin Microb Rev. 2006;19(1):11-26.

Mandeel QA, Al-Laith AA. Ethnomycological aspects of the desert truffle among native Bahraini and non-Bahraini peoples of the Kingdom of Bahrain. J Ethnopharmacol. 2007;110:118-29.

Lee KW, Lee HJ, Lee CY. Vitamins, phytochemicals, diets, and their implementation in cancer chemoprevention. Critical Rev Food Sci Nut. 2004;44:437-52.

Bazzano LA, Serdula MK, Liu S. Dietary intake of fruits and vegetables and risk of cardiovascular disease. Curr Athero Rep. 2003;5:492-9.

Sherry E, Sivananthan S, Warnke PH, Eslick GD. Topical phytochemicals used to salvage the gangrenous lower limbs of type 1 diabetic patients. Diabetes Res Clin Prac. 2003;62:65-6.

Mahmoudvand H, Saedi Dezaki E, Ezatpour B, Sharifi I, Kheirandish F, Rashidipour M. In vitro and In vivo antileishmanial activities of Pistacia vera essential Oil.Planta Medica. 2016;82(4):279-84.

Adams RP. Identification of essential oil components by gas chromatography/mass spectroscopy. 2004; Illinois, USA: Carol Stream, IL: Allured Publishing Corporation.

Cowan MM. Plant products as antimicrobial agents. Clin Microb Rev. 1999;12:564-82.

McCutcheon AR, Ellis SM, Hancock REW, Tower GNH. Antibiotic screening of medicinal plants of the British Columbian native peoples. J Ethnopharmacol. 1992;37:213-23.

Lai PK, Roy J. Antimicrobial and chemopreventive properties of herbs and spices. Curr Med Chem. 2004,11:1451–60.

Kheirandish F, Delfan B, Farhadi S, Ezatpour B, Khamesipour A, Kazemi B, Ebrahimzade F, Rashidipour M. The effect of Satureja khuzestanica essential oil on the lesions induced by Leishmania major in BALB/c mice. African J Pharm Pharmacol. 2011;5(5):648-53.

Moshayedi SH, Shahraz F, Schaffner DW, Khanlarkhani A, Shojaee-Aliabadi S, Shahnia M, et al. In vitro control of Enterococcus faecalis by Zataria multilfolira Boiss, Origanum vulgare L and Mentha pulegium essential oils. J Food Safety. 2013;1745-4565.

de Melo JO, Bitencourt TA, Fachin AL, Cruz EM, de Jesus, HC, Alves PB, et al. Antidermatophytic and antileishmanial activities of essential oils from Lippia gracilis Schauer genotypes. Acta Trop. 2013;128(1):110-5.

Monzote L, García M, Pastor J, Gil L, Scull R, Maes L, Cos P, Gille L. Essential oil from Chenopodium ambrosioides and main components: activity against Leishmania, their mitochondria and other microorganisms. Exp Parasitol. 2014;136:20-6.

Mahboubi M, Qazian Bidgoli F. Chemical composition and anti¬microbial activity of Artemisia aucheri Boiss. Essential oil Med Aroma plant J. 2009;25(3):429-40.




DOI: https://doi.org/10.22087/hmj.v1i1.557

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Lorestan University of Medical Sciences, Khorramabad, Iran.

ISSN: 2538-2144