Chemical Composition, Antioxidant and Antimicrobial Activities of Essential Oil from Leutea kurdistanica mozaff

Naser Karimi, Iraj Salimikia*, Parvin Ramak, Zhaleh Soheilikhah, Mohammad Shamizadeh, Mohammad Bagher Gholivand

Abstract


Background and Aim: Leutea kurdistanica mozaff. (Apiaceae) is a perennial herb endemic to Kurdistan province of Iran. The plants of this family are known for diversity in essential oils and economic importance. The goals of the present study were to evaluate chemical composition, antioxidant and antimicrobial activities of essential oils of L. kurdistanica.

Materials and Methods: GC-FID and GC-MS were used for compositional analysis of the essential oils.Antioxidant activities of the oil were assessed using DPPH free radical test. Antibacterial and antifungal activities of the oils were assessed by disc diffusion and agar dilution methods.

Results: A total of 33 compounds representing 97.3-94.2% of the essential oils were identified. Limonene (25.3%) was the main compound of the oil followed by γ–terpinene (18.1%), elemicin (15.4%) and Δ-3-carene (8.2%). In DPPH assay, the oil has radical scavenging activity with IC50 value of 51.4±2.3. The results of antimicrobial tests showed that the oil had remarkable inhibitory effects on the growth of Gram-positive bacteria and fungal strains.

Conclusions: Regarding to significant antioxidants and antimicrobial activities of the oil of L. kurdistanica, these oils could be consider as a natural preservative in food and other industries. Although more investigation is needed to clarify the exact compounds responsible for observed biological activities.


Keywords


Antimicrobial; Antioxidant; Essential oil; Leutea kurdistanica mozaff

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References


Mozaffarian V. Flora of Iran: Umbelliferae. 1st ed. Tehran: Research Institute of Forests and Rangelands; 2007.

Booker A, Johnston D, Heinrich M. Value chains of herbal medicines Research needs and key challenges in the context of ethnopharmacology. J Ethnopharmacol. 2012;140(3):624-33.

Shahat AA, Ibrahim AY, Hendawy SF, Omer EA, Hammouda FM, Abdel-Rahman FH, et al. Chemical composition, antimicrobial and antioxidant activities of essential oils from organically cultivated fennel cultivars. Molecules. 2011;16(2):1366-77.

Abas F, Khatib A, Shaari K, Lajis NH. Chemical characterization and antioxidant activity of three medicinal Apiaceae species. Ind Crops Prod. 2014;55:238-47.

Oroojalian F, Kasra-Kermanshahi R, Azizi M, Bassami M. Phytochemical composition of the essential oils from three Apiaceae species and their antibacterial effects on food-borne pathogens. Food Chem. 2010;120(3):765-70.

Rechinger K. Flora Iranica. 1sted. Graz, Austria: Akademische Druck und Verlagsanstalt; 1982.

Masoudi S, Rustaiyan A, Ameri N. Volatile Oils of Ferulago phialocarpa Rech. f. et H. Reidl. and Leutea elbursensis Mozaff. from Iran. J Essent Oil Res. 2004;16(2):143-4.

Yassa N, Akhani H, Aqaahmadi M, Salimian M. Essential oils from two endemic species of Apiaceae from Iran. Z Naturforsch C. 2003;58(7-8):459-63.

Faraji R, Bigdelo M, Rezaei K, Hooshidari F, Mirzaei HH. Essential oil composition of Leutea kurdistanica (Mozaff.) at the vegetative and flowering stages. J Essent Oil Bear Pl. 2016;19(1):223-8.

Adams RP. Identification of essential oil components by gas chromatography/mass spectrometry. J Am Soc Mass Spectrom. 1997;8(6):671-2.

Salimikia I, Yazdinezhad AR, Golfakhrabadi F, Esfahani HRM. In vitro antioxidant and free radical scavenging activity of four Alkanna species growing in Iran. Pharmacogn Res. 2015;7(1):100-4.

Schlesier K, Harwat M, Böhm V, Bitsch R. Assessment of antioxidant activity by using different in vitro methods. Free Radical Res. 2002;36(2):177-87.

Andrews J, Jennifer M. BSAC standardized disc susceptibility testing method. J Antimicrob Chemother. 2001;48(1):43-57.

Zaidan M, Noor Rain A, Badrul A, Adlin A, Norazah A, Zakiah I. In vitro screening of five local medicinal plants for antibacterial activity using disc diffusion method. Trop Biomed. 2005;22(2):165-70.

Reller LB, Weinstein M, Jorgensen JH, Ferraro MJ. Antimicrobial susceptibility testing: a review of general principles and contemporary practices. Clin Infect Dis. 2009;49(11):1749-55.

Sarker SD, Nahar L, Kumarasamy Y. Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods. 2007;42(4):321-4.

Sangwan NS, Farooqi AH, Shabih F, Sangwan RS. Regulation of essential oil production in plants. Plant Growth Regul. 2001;34(1):3-21.

Ruberto G, Baratta MT. Antioxidant activity of selected essential oil components in two lipid model systems. Food Chem. 2000;69(2):167-74.

Foti MC, Ingold K. Mechanism of inhibition of lipid peroxidation by γ-terpinene, an unusual and potentially useful hydrocarbon antioxidant. J Agri Food Chem. 2003;51(9):2758-65.

Misharina T, Samusenko A. Antioxidant properties of essential oils from lemon, grapefruit, coriander, clove, and their mixtures. Appl Biochem Microbiol. 2008;44(4):438-42.

Dorman H, Deans S. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol. 2000;88(2):308-16.

Van Vuuren S, Viljoen A. Antimicrobial activity of limonene enantiomers and 1, 8‐cineole alone and in combination. Flav Frag J. 2007;22(6):540-4.

Settanni L, Palazzolo E, Guarrasi V, Aleo A, Mammina C, Moschetti G, et al. Inhibition of foodborne pathogen bacteria by essential oils extracted from citrus fruits cultivated in Sicily. Food Control. 2012;26(2):326-30.

Roberto D, Micucci P, Sebastian T, Graciela F, Anesini C. Antioxidant activity of limonene on normal murine lymphocytes: relation to H2O2 modulation and cell proliferation. Basic Clin Pharmacol Toxicol. 2010;106(1):38-44.

Burt S. Essential oils: their antibacterial properties and potential applications in foods a review. Int J Food Microbiol. 2004;94:223-53.

Helander IM, Alakomi H-L, Latva-Kala K, Mattila-Sandholm T, Pol I, Smid EJ, et al. Characterization of the action of selected essential oil components on Gram-negative bacteria. J Agri Food Chem. 1998;46(9):3590-5.

De Almeida Freires I, Murata RM, Furletti VF, Sartoratto A, de Alencar SM, Figueira GM, et al. Coriandrum sativum L.(coriander) essential oil: antifungal activity and mode of action on Candida spp., and molecular targets affected in human whole-genome expression. Plos One. 2014;9(6):86-99.

Hossain F, Follett P, Salmieri S, Vu K, Harich M, Lacroix M. Evidence for synergistic activity of plant-derived volatile essential oils against fungal pathogens of food. Food Control. 2015;45:156-62.

Knobloch K, Pauli A, Iberl B, Weigand H, Weis N. Antibacterial and antifungal properties of essential oil components. J Essent Oil Res. 1989;1(3):119-28.

Filipowicz N, Kamiński M, Kurlenda J, Asztemborska M, Ochocka JR. Antibacterial and antifungal activity of juniper berry oil and its selected components. Phytother Res. 2003;17(3):227-31.

Pinto E, Pina-Vaz C, Salgueiro L, Gonçalves MJ, Costa-de-Oliveira S, Cavaleiro C, et al. Antifungal activity of the essential oil of Thymus pulegioides on Candida, Aspergillus and dermatophyte species. J Med Microbiol. 2006;55(10):1367-73. doi: 10.1099/jmm.0.46443-0

Sekine T, Sugano M, Majid A, Fujii Y. Antifungal effects of volatile compounds from black zira (Bunium persicum) and other spices and herbs. J Chem Ecol. 2007;33(11):2123-32.




DOI: https://doi.org/10.22087/hmj.v1i1.564

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Lorestan University of Medical Sciences, Khorramabad, Iran.

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