The Effects of Olive Leaf Extract Administration on the Cerebral Hypoperfusion-Induced Electrophysiological Alterations in Rat Heart
Abstract
Background and Aim: In the present study, the role of ethanolic extract of olive (Olea europaea) leaf administration against the cerebral hypoperfusion-induced electrophysiological alterations and left ventricular thickness in rat heart was studied.Materials and Methods: The right carotid artery was occluded with a 5-0 silk suture and the left carotid artery was occluded after a week. The olive (Olea europaea) leaf extract was administered orally (by gavage) to three experimental groups of rats in 100, 200 and 300 mg/kg/day for two weeks after the left carotid artery occlusion and processed in 25 days. The sham and hypoperfusion groups were fed with normal saline. Each group had 6 rats. After 25 days, electrophysiological heart parameters, i.e. HR, QTc, QRS duration, QT interval, PR interval, RR interval, ST amplitude, T wave amplitude and also the thickness of left ventricle (TLV) were assessed.Results: The administration of olive leaf extract (100, 200 and 300 mg/kg) exhibited its cardioprotective effect by both stabilizing heart rate and preventing ECG changes (such as shortening of PR, QRS, QT and QTc intervals, prominent T waves and ST elevation). Moreover, olive leaf extract (100, 200 and 300 mg/kg) prevented cardiac hypertrophy after cerebral hypoperfusion.Conclusion: Our results indicated that post-treatment with ethanolic extract of olive leaves could prove beneficial effects in managing the cerebral hypoperfusion-induced cardiac abnormality in rats.References
van der Bilt IA, Hasan D, Vandertop WP, Wilde AA, Algra A, Visser FC, et al. Impact of cardiac complications on outcome after aneurysmal subarachnoid hemorrhage: a meta-analysis. Neurology. 2009;72(7):635-42.
Zygun D. Non-neurological organ dysfunction in neurocritical care: impact on outcome and etiological considerations. Curr Opin Crit Care. 2005;11(2):139-43.
Gregory T, Smith M. Cardiovascular complications of brain injury. Continuing Education in Anaesthesia, Critical Care & Pain. 2012;12(2).
Barnett HJ. Carotid disease and cognitive dysfunction. Ann Intern Med. 2004;140(4):303-4.
Nguyen H, Zaroff JG. Neurogenic stunned myocardium. Curr Neurol Neurosci Rep. 2009;9(6):486-91.
Tung P, Kopelnik A, Banki N, Ong K, Ko N, Lawton MT, et al. Predictors of neurocardiogenic injury after subarachnoid hemorrhage. Stroke. 2004;35(2):548-51.
El Sedef N, Karakaya S. Olive tree (Olea europaea) leaves: potential beneficial effects on human health. Nutrition reviews. 2009;67(11):632-8.
Andreadou I, Iliodromitis EK, Mikros E, Constantinou M, Agalias A, Magiatis P, et al. The olive constituent oleuropein exhibits anti-ischemic, antioxidative, and hypolipidemic effects in anesthetized rabbits. The journal of nutrition. 2006;136(8):2213-9.
Singh I, Mok M, Christensen A-M, Turner AH, Hawley JA. The effects of polyphenols in olive leaves on platelet function. Nutrition, metabolism and cardiovascular diseases. 2008;18(2):127-32.
Wang L, Geng C, Jiang L, Gong D, Liu D, Yoshimura H, et al. The anti-atherosclerotic effect of olive leaf extract is related to suppressed inflammatory response in rabbits with experimental atherosclerosis. European journal of nutrition. 2008;47(5):235-43.
Mohagheghi F, Bigdeli MR, Rasoulian B, Hashemi P, Pour MR. The neuroprotective effect of olive leaf extract is related to improved blood-brain barrier permeability and brain edema in rat with experimental focal cerebral ischemia. Phytomedicine. 2011;18(2-3):170-5.
Rafighdoost Houshang, Tavafi Majid, Jafari pour Leila, Rasoulian Bahram , Ahmadvand Hassan , Mahmodi Mona , et al. Effect of Olive Leaf Extract inInhibition of Renal Ischemia- Reperfusion Injuries in Rat. ASJ. 2013;10 (3):160-5.
M A, O D, A K, Sh N, A K, A P. Hepatoprotective effects of purified oleuropein from olive leaf extract against ethanol-induced damages in the rat. The Iranian Journal of Veterinary Research. 2012;13(3):218-26.
Leila J, Bahram R, Majid T, Houshang R, Mona M, Marzyeh R, et al. Pretreatment with Olive Leaf Extract Improves Renal and Liver Antioxidant Systems Following Renal Ischemia-Reperfusion Injury in Rats. Herbal Medicines Journal 2016;1(1):37-46
Rezvan V, Nariman M, SeyedJavadSeyed T, Farnaz K, Javad TM, Zahra A-M, et al. Effect of Olive Leaf Extract on Cytokines Secreted by Macrophage. Novelty in Biomedicine. 2016;4(3):116-20(5).
Ayat K, Maryam R, Marzieh r. Oleuropein Reduces Cisplatin-Induced Nephrotoxicity in Human Embryonic Renal Epithelial “GP-293” Cells. Herbal Medicines Journal. 2016;1:18-23.
Esmailidehaj M, Mirhosseini SJ, Rezvani ME, Rasoulian B, Mosaddeghmehrjardi MH, Haghshenas D. Prolonged Oral Administration of Oleuropein Might Protect Heart against Aconitine-induced Arrhythmia. Iran J Pharm Res. 2012;11(4):1255-63.
Mansour E, Shirin B, Ebrahim RM, Mozhdeh S, Zahra H, Zeynab H. Effect of oleuropein on myocardial dysfunction and oxidative stress induced by ischemic-reperfusion injury in isolated rat heart. Journal of Ayurveda and Integrative Medicine. 2016;7(4):224-30.
Marzieh R, Heydari R, Feizbakhsh A, Hashemi P. Rapid Screening of Oleuropein from Olive Leaves Using Matrix Solid-Phase Dispersion and High-Performance Liquid Chromatography. Journal of AOAC International 2014;97(4):1109-13.
Mann DL, Kent RL, Parsons B, Cooper Gt. Adrenergic effects on the biology of the adult mammalian cardiocyte. Circulation. 1992;85(2):790-804.
Ruthirago D, Julayanont P, Tantrachoti P, Kim J, Nugent K. Cardiac Arrhythmias and Abnormal Electrocardiograms After Acute Stroke. Am J Med Sci. 2016;351(1):112-8.
Selvetella G, Notte A, Maffei A, Calistri V, Scamardella V, Frati G, et al. Left ventricular hypertrophy is associated with asymptomatic cerebral damage in hypertensive patients. Stroke. 2003;34(7):1766-70.
Nekooeian AA , Dehghani GA, Mostafavi H, Khalili A. The Effect of Hydroalcoholic Extract of Olive Leaves on Blood Pressure in Rat Model of Two-Kidney, One-Clip Goldblatt Hypertension. Iran Cardiovasc Res J. 2011;5(1):1-6.
Somova LI, Shode FO, Mipando M. Cardiotonic and antidysrhythmic effects of oleanolic and ursolic acids, methyl maslinate and uvaol. Phytomedicine. 2004;11(2-3):121-9.
Molojavyi A, Preckel B, Comfere T, Mullenheim J, Thamer V, Schlack W. Effects of ketamine and its isomers on ischemic preconditioning in the isolated rat heart. Anesthesiology. 2001;94(4):623-9; discussion 5A-6A.
Waterman E, Lockwood B. Active components and clinical applications of olive oil. Altern Med Rev. 2007;12(4):331-42.
Esmailidehaj M, Rasulian B, Rezvani ME, Delfan B, Mosaddeghmehrjardi MH, Pourkhalili K. The anti-infarct, antistunning and antiarrhythmic effects of oleuropein in isolated rat heart. EXCLI J. 2012;11:150-62.
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