The Antimicrobial Activity of Different Extracts from Echinophora platyloba DC.

Leili Bazvandi, Yalda Shokoohinia, Nastaran Ghiasvand, Parviz Mohajeri, Behnam Ashrafi, Iraj Salimikia*

Abstract


Background and Aim: Echinophora platyloba DC (Apiaceae) is a plant endemic to Kurdistan province of Iran. Aerial part of the plant is regarded as the source of natural antimicrobial agent.

Materials and Methods: The aerial part of E. platyloba was extracted with hexane, dichloromethane, acetone EtOH and EtOH: H2O respectively. The extracts were individually tested against three gram-negative (Escherichia coli, Shigella flexneri, Acinetobacter baumannii) and two gram-positive bacteria (Staphylococcus aureus, Enterococcus faecalis) via agar dilution methods.

Results: The best inhibitory effect was observed in ethanolic extract, which had a remarkable inhibitory effect on all bacteria especially on S. aureus. The results also indicated that dichloromethane extract showed the weakest inhibitory effect on all types of bacteria. Meanwhile, acetone extract exhibited a relatively mild inhibitory effect.

Conclusion: The antimicrobial effects of E. platyloba confirmed its potential for folk use. More comprehensive investigations are recommended concerning the significant antibacterial activity of the ethanol extract of E. platyloba to determine the exact compounds responsible for observed biological activities.


Keywords


Echinophora platyloba DC., Extracts, Antimicrobial

Full Text:

PDF

References


Rates SM. Plants as source of drugs. Toxicon. 2001;39(5):603-13.

Mazloomifar H, Saber-Tehrani M, Rustaiyan A, Masoudi S. Constituents of the Essential Oil of Echinophora platyloba DC. Growing Wild in Iran. Journal of Essential Oil Research. 2004;1:16(4).

Rahimi-Nasrabadi M, Gholivand MB, Niasari M, Vatanara A. Chemical composition of the essential oil from aerial parts of Echinophora platyloba DC. from Iran. Journal of Medicinal Plants. 2010;15;1(33):53-6.

Saei‐Dehkordi SS, Fallah AA, Saei‐Dehkordi SS, Kousha S. Chemical Composition and Antioxidative Activity of Echinophora platyloba DC. Essential oil, and its interaction with natural antimicrobials against food‐borne pathogens and spoilage organisms. Journal of food science. 2012;77(11):M631-M7.

Hussain AI, Anwar F, Sherazi STH, Przybylski R. Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food chemistry. 2008;108(3):986-95.

Karimi N, Salimikia I, Ramak P, Soheilikhah Z, Shamizadeh M, et al.Chemical Composition, Antioxidant and Antimicrobial Activities of Essential Oil from Leutea kurdistanica mozaff. Herbal Medicine Journal. 2016;1(1):47-52.

Alzamora SM, Guerrero S, López-Malo A, Palou E, Roller S. Plant antimicrobials combined with conventional preservatives for fruit products. Natural antimicrobials for the minimal processing of foods. 2003:235-49.

Cowan MM. Plant products as antimicrobial agents. Clinical microbiology reviews. 1999;12(4):564-82.

Asghari GR, Sajjadi SE, Sadraei H, Yaghobi KH. Essential oil constituents of Echinophora platyloba DC. Iranian Journal of Pharmaceutical Research. 2010; 20:185-6.

Andrews JM. Determination of minimum inhibitory concentrations. Journal of antimicrobial Chemotherapy. 2001; 1;48( 1):5-16.

Kim HY, Lee YJ, Hong KH, Kwon YK, Sim KC, Lee JY, Cho HY, Kim IS, Han SB, Lee CW, Shin IS. Isolation of antimicrobial substances from natural products and their preservative effect. Food Science and Biotechnology. 2001 Feb;10(1):59-71.

Majid A, Mohaddesse M, Mahdi D, Mahdi S, Sanaz S, Kassaiyan N. Overview on Echinophora platyloba, a synergistic anti-fungal agent candidate. Journal of Yeast and Fungal research. 2010 Jul 31;1(5):88-94.

Asghari G, Abedi D, Jalali M, Farsi S. Antimicrobial activities and phytochemical composition of Echinophora platyloba DC. essential oils from Isfahan. Journal of Essential Oil Bearing Plants. 2007;10(1):76-82.

Entezari M, Hashemi M, Ashki M, Ebrahimian S, Bayat M, Azizi Saraji A, et al. Studying the effect Echinophora platyloba extract on bactira (Staphilococus aureus and Pseudomonas aeruginosa) and fungi (Candidia albicans, Aspergilus flavus and Aspergilus niger) in vitro. World Journal of Medical Sciences. 2009;4(2):89-92.

Hashemi M, Ehsani A, Jazani NH, Aliakbarlu J, Mahmoudi R, editors. Chemical composition and in vitro antibacterial activity of essential oil and methanol extract of Echinophora platyloba DC against some of food-borne pathogenic bacteria. Veterinary Research Forum. 2013; 4(2) 123-127.

Aligiannis N, Kalpoutzakis E, Mitaku S, Chinou IB. Composition and antimicrobial activity of the essential oils of two Origanum species. Journal of agricultural and food chemistry. 2001 Sep 17;49(9):4168-70.

Nikaido H. Molecular basis of bacterial outer membrane permeability revisited. Microbiology and molecular biology reviews. 2003;67(4):593-656.

Costerton JW, Ingram JM, Cheng KJ. Structure and function of the cell envelope of gram-negative bacteria. Bacteriological reviews. 1974 Mar;38(1):87.

Jacobs C, Frère JM, Normark S. Cytosolic intermediates for cell wall biosynthesis and degradation control inducible β-lactam resistance in gram-negative bacteria. Cell. 1997 Mar 21;88(6):823-32.

Celikel N, Kavas G. Antimicrobial properties of some essential oils against some pathogenic microorganisms. Czech journal of food sciences. 2008;26(3):174-81.

Mahmoodi KF, Alizadeh Z, Bahadori MB. Isolation and structure elucidation of secondary metabolites from Echinophora platyloba DC from Iran. Journal of Medicinal Plants. 2014 Mar 15;1(49):15-21.




DOI: https://doi.org/10.22087/hmj.v0i0.655

Refbacks

  • There are currently no refbacks.


This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge.

                               

This work is licensed under a Creative Commons license (CC-BY).  However, the license permits any user to read, copy, redistribute and and make derivative the material in any medium or format for any purpose, even commercially.


 

Lorestan University of Medical Sciences, Khorramabad, Iran.

ISSN: 2538-2144