Assessing the Subchronic Toxicity of the Ethanolic Extract of the Pods of Tetrapleura tetraptera on Swiss Albino Mice

Oloyede Michael Adeola *


Background and Aim: Tetrapleura tetraptera fruit is widely used in south-east and western Nigeria not only as a spice but also as a remedy for various ailments. This research aims at examining the subchronic toxicity of the ethanolic extract of the pods of T. tetraptera on Swiss albino mice.

Materials and Methods: Twenty-five Swiss albino Wistar mice weighing between 18-30g were randomly divided into five groups. Animals in group 1 that was regarded as the control group were administered distilled water. Animals in group 2-4 were orally administered 50,100 and 200mg kg-1 bodyweight of T. tetraptera. All animals were fasting on the eve of the 43rd day. Following the withdrawal of treatment, the blood, liver, kidneys, lungs and heart were removed for hematologic, biochemical and histologic investigations.

Results: No remarkable alteration was observed in the hematologic parameters except for the significant reduction in the WBC. The biochemical investigation indicated gross changes in most of the biochemical analytes dose-dependently. The levels of ALT, ALP, TB, DB, PRO, CREA, UREA, ALB and CHOL were all noticeably (P˂0.05) different from the control group in all doses which might be indicative of toxicity. Moreover, some minor changes were observed in the histoarchitecture of the liver and the kidney.

Conclusion: Hence, the results revealed that the extract might have induced toxicity to the kidneys and liver which may in turn influence other parts of the body. Tetrapleura tetraptera may contain some phytochemicals, which on prolonged use may affect the physiology of the body.


Tetrapleura tetraptera, Subchronic toxicity, Hematology, Histology, Biochemical analytes

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Nwoba EG. Proximate and Phytochemical Composition of the Pulp of Tetrapleura tetraptera Fruits Consumed in Abakaliki, Nigeria. International Journal of Engineering Research & Technology (IJERT). 2015;4(6):1286-94.

Adesina SK, Iwalewa EO, Johnny I. Tetrapleura tetraptera Taub- Ethnopharmacology, Chemistry, Medicinal and Nutritional Values-A Review British Journal of Pharmaceutical Research. 2015;12(3):1-22.

Salahdeen HM, Omoaghe AO, Isehunwa GO, Murtala BA, Alada AR. Gas chromatography mass spectrometry (GC-MS) analysis of ethanolic extracts of kolanut (Cola nitida) (vent) and its toxicity studies in rats. Journal of Medicinal Plants Research. 2015;9(3):56-70.

Adeleye SA, Oguoma OI, Braide W, Osogbase EE. Phytochemistry and Antimicrobial Properties of 2:1 and 1:2 Ethanol-methanol Extracts of Tetrapleura tetraptera. American Journal of Current Microbiology. 2016;4(1).

Tsala DE, Habtemariam S, Simplice FH, Ndzana MTB, Jital AA, Dimo T. Topically Applied Tetrapleura tetraptera Stem-Bark Extract Promotes Healing of Excision and Incision Wounds in Rats. Journal of Intercultural Ethnopharmacology. 2014;3(2):63-7.

Onuka AE, Okechukwu NC, Maxine, KM. Evaluation of the Effects of Tetrapleura tetraptera Extract and Clomiphene Citrate to Determine Influence of Reproductive Hormones and Estrous Cycle on Leukocyte Counts: Novel Evidence for a Potential Developmental Link Between Luteinizing Hormone and MID Cells. Hematology & Transfusion International Journal. 2017;5(6).

Ishola IO, Afolayan GO, Olugbade IO, Olasunmbo Owolabi-Afolayan O. Protective effect of Tetrapleura tetraptera (Schum. & Thonn.) fruit extract against haloperidol-induced catalepsy and scopolamine-induced memory impairment: Involvement of antioxidant system. West African Journal of Pharmacology and Drug Research. 2015;30.

Uyoh EA, Ita EE, Nwofia GE. Evaluation of the chemical composition of Tetrapleura tetraptera (Schum and Thonn.)Taub. Accessions from Cross River state, Nigeria. International Journal of Medicinal and Aromatic plants. 2013;3(3):386-94.

Bauter M, Mendes O. Subchronic toxicity of lyophilized apoaequorin protein powder in Sprague-Dawley rats. Toxicology Research and Application. 2018;2:1-15.

Madingou NOK, Traore A, Souza A, Mounanga MMB, Samseny R, Sylvin Ouedraogo S. Preliminary studies of acute and sub-chronic toxicity of the aqueous extract of Guibourtia tessmannii (Harms) J. Leonard stem barks (Caesalpiniaceae) in mice and rats. Asian Pacific Journal of Tropical Biomedicine. 2016;6(6):506-10.

Ouedraogo S, Some N, Ouattara S, Kini FB, Traore A, Bucher B. Acute toxicity and vascular properties of seed of Parkia biglobosa (JACQ) R. Br Gift (Mimosaceae) on rat aorta. African Journal of Traditional and Complementary Alternative Medicine. 2011;9(2):260-5.

Gautam MK Goel RK. Toxicological study of Ocimum sanctum Linn leaves: hematological, biochemical, and histopathological studies. Journal of Toxicology Article. 2014. ID 135654,

Orong LX, Yongjiang L, Lijuan W, Yuhan LG, Xue SY, Cui Y, Xue M. Acute and subacute toxicity of ethanolic extracts from Salvia przewalskii Maxim in rodents. Journal of Ethnopharmacology. 2010;131:110-5.

Humphrey Adebayo A, Cecilia Ogundare O, Samuel Adegbite O. Sub-Acute Evaluation of Extract of Syzygium malaccense in Albino Rats. Research Journal of Medicinal Plant. 2015;9(2):60-71.

Kasper DL, Braunwald E, Fauci AS, Hauser SL, Longo DL. Harrison's Principles of Internal Medicine. 2005; 16th Edn., McGrow-Hill, New York, USA.

Adebayo AH, OS, Adegbite JA, Olugbuyiro OO. Toxicological evaluation of extract of Olax subsorpioidea on albino Wistar rats. African Journal of Pharmarcy and Pharmacology. 2014;8:570-8.

Odeyemi OO, Yakubu MT, Masika PJ, Afolayan AJ. Toxicological evaluation of the essential oil from Mentha longifolia L. subsp. capensis leaves in rats. Journal of Medicinal Food. 2009;12(3):669-74.

Das N, Goshwami D, Hasan S, Raihan SZ. Evaluation of acute and subacute toxicity induced by methanol extract of Terminalia citrina leaves in Sprague Dawley rats. Journal of Acute Disease. 2015;4(4):316-21.

Odeghe OB, Uwakwe AA, Monago CC. Some biochemical and haematological studies on the methanolic extract of Anthocleista grandiflora stem bark. International Journal of Applied Science and Technology. 2012;2(5):58-65.

Ferreira SA, Guimarães AG, Ferrari FC, Martins Carneiro C, Nogueira de Paiva NC, Guimarães DA. Assessment of acute toxicity of the ethanolic extract of Lychnophora pinaster (Brazilian arnica) Revista Brasileira de Farmacognosia. 2014;24(5).

Harizal SN, Mansor SM, Hasnan J, Tharakan JK, Abdullah J. Acute toxicity study of the standardized methanolic extract of Mitragyna speciosa Korth in rodent. Journal of Ethnopharmacology. 2010;131:404-9.

Chang CJ, Tzeng TF, Liou SS, Chang YS, Liu IM. Acute and 28-day subchronic oral toxicity of an ethanol extract of Zingiber zerumbet (L.) Smith in rodents. Evidence Based Complementary and Alternative Medicine. 2012;608284.

Konate K, Bassol´e IHN, Hilou A, Aworet-Samseny RRR, Souza A, Barro N, Mamoudou HD, Jacques Y. Toxicity assessment and analgesic activity investigation of aqueous acetone extracts of Sida acuta Burn f. and Sida Cordifolia L. (Malvaceae), medicinal plants of Burkina Faso. BMC Complementary and Alternative Medicine. 2012;12:120.

Filippin FB, Reis K, Cemin L, Duzzioni M, Hermes EM, Souza LC. Novo intervalo de referência para alanina aminotransferase usando o sistema automatizado de bioquímica Dade Behring Ar Dimension. NewsLab. 2004;65:148-60.

Kew MC. Serum aminotransferase concentration as evidence of hepatocellular damage. Lancet. 2000;355:591-2.

Allman MA, Pang E, Yau DF, Stewart PM, Tiller DJ, Truswel AS. Elevated plasma vitamers of vitamin B6 in patients with chronic renal failure on regular haemodialysis. European Journal of Clinical Nutrition. 1992;46:679-83.

Saidu Y, Bilbis LS, Lawal M, Isezuo SA, Hassan SW, Abbas AY. Acute and sub-chronic toxicity studies of crude aqueous extract of Albizzia chevalieri harms (Leguminosae). Asian Journal of Biochemistry. 2007;2:224-36.



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Lorestan University of Medical Sciences, Khorramabad, Iran.

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