A Comparison of the Effects of the Hydro-Alcoholic and Aqueous Extracts of Aloe Vera on the Proliferation of MCF7

  • Leila Zarei * Department of Anatomical Sciences, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Seyyed Meysam Abtahi Froushani Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Neda Eslami Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Mohammed Abkhaz Department of Microbiology, School of Veterinary Sciences, Urmia University, Urmia, Iran
Keywords: Hydroalcoholic, Aloe Vera, Breast cancer, MCF7

Abstract

Background: Breast cancer is the most common cancer and the second leading cause of cancer death among women. Regarding that chemotherapy drugs are often associated with adverse effects on the patients. Common medicinal therapies for breast cancer have often short term effects and some of them lead to drug resistance. The target of this study is to determine the effects of aqueous and hydro-alcoholic extract of Aloe Vera on MCF 7 cells.   Materials and Methods: After culturing MCF7 cancer cells in the PRMI 1460 medium, both aqueous and hydro-alcoholic extract of Aloe Vera was prepared in concentrations of 20, 40, 60, 80 and 100 μg/mL from bulk solution. Then MCF7 cells were contacted with different concentration of prepared extract. The MTT test and Acridine Orange / Propidium Iodide (AO/PI) stained was used to determine the survival rate of cells. Doxorubicin (0.1 Mm) was used as positive control. The T-Student test was used in order to analyze the data using SPSS 21.  p< 0.05 was considered as significant level.  Result: The results of MTT assay and viability showed that different concentrations of ethanol extracts of Aloe Vera plant to the concentration of the extract significantly and dose-dependently decreased cell proliferation of MCF7 gets (p < 0.05).   Conclusion: It seems should be considered that hydro alcoholic extract can be used as a natural compound in the treatment of breast cancer.

References

Silva MJD, Carvalho AJS, Rocha CQ, Vilegas W, Silva MA, Gouvea CMCP. Ethanolic extract of Mimosa caesalpiniifolia leaves: Chemical characterization and cytotoxic effect on human breast cancer MCF-7 cell line. S. Afr. J. Bot. 2014;93:64-9.

Sriwiriyajan S, Ninpesh T, Sukpondma Y, Nasomyon T, Graidist P. Cytotoxicity screening of plants of genus Piper in breast cancer cell lines. Trop. J. Pharm. Res. 2014;13:921-8.

De Rezende AA, Munari C.C, de Oliveira P.F, Ferreira N.H, Tavares D.C, E Silva M.L.A, et al. A comparative study of the modulatory effects of (−)-cubebin on the mutagenicity/recombinogenicity induced by different chemical agents. Food Chem. Toxicol. 2013;55:645-52.

Nahak G, Sahu R.K. Phytochemical evaluation and antioxidant activity of Piper cubeba and Piper nigrum. J. Appl. Pharm. Sci. 2011;1:153-7.

Soule HD, Vazquez J, Long A, Albert S, Brennan M. A human cell line from a pleural effusion derived from a breast carcinoma". Journal of the National Cancer Institute. 1973; 51(5):1409-16.

Perazzo F.F, Rodrigues I.V, Maistro E.L, Souza S.M, Nanaykkara N.P.D, Bastos J.K, et al. Anti-inflammatory and analgesic evaluation of hydroalcoholic extract and fractions from seeds of Piper cubeba L. (Piperaceae). Pharmacogn. J. 2013;5:13-6.

Bush J, van den Boom R, Franklin SH.Comparison of aloe vera and omeprazole for treatment of equine gastric ulcer syndrome. Vet J. 2017;28:255-84.

Sharrif Moghaddasi M. Aloe vera Chemicals and Usages. Advances in Environmental Biology. 2010;4(3):464-8.

Sharrif Moghaddasi M, Res M. Aloe vera their chemicals composition and applications: a review. Int J Biol Med Res. 2011;2(1):466-71.

Fusco D, Liu X, Savage C, Taur Y, Xiao W, Kennelly E, et al. Echinacea purpurea aerial extract alters course of influenza infection in mice.J Vaccine. 2010;28(23):3956-62.

Abtahi Froushani SM, Esmaili gouvarchin Galee H, Khamis abadi M, Lotfallahzade B. Immunomudulatory effects of hydroalcoholic extract of Hypericumperforatum. Avicenna J Phytomed. 2015;5(1):62-8.

Abtahi Froushani SM, Esmaili gouvarchin H, Mansouri Motlagh B, Babaei M. Q Horizon Med Sci. 2014; 20(3):166-70.

Jabbari N, Zarei L, Esmaili gouvarchin H, Mansouri Motlagh B. Artificial cells, Nanomedicine and Biotechnology. 2018;1-9.

Turina M, Miller FN, McHugh PP, Cheadle WG, Polk HC Jr. Endotoxin inhibits apoptosis but induces primary necrosis in neutrophils. Inflammation. 2005;29:55-63.

Kumar KS, Bhowmik D, Biswajit C. Aloe vera: a potential herb and its medicinal importance. J Chem Pharm Res. 2010;2:21-9.

Hamid AAA, Soliman MF. Effect of topical aloe vera on the process of healing of full-thickness skin burn: a histological and immunohistochemical study. Journal of Histology & Histopathology. 2015;2(1):3.

Carvalho A.J.S, Ishikawa T, Gouvea C.M.C.P. Aqueous extract of Plinia edulis leaves:Antioxidant activity and cytotoxicity to human breast cancer MCF-7 cell line. S. Afr. J. Bot. 2012;81:1-7.

Siegel R, Ma J, Zou Z, Jemal A. Cancer Statistics, 2014. CA: Cancer J. Clin. 2014;64:9-29.

Kai-Yuanlin Y. Aloe-emodin, an anthraquinone, in vitro inhibits proliferation and induces apoptosis in human colon carcinoma cells. Oncology Letters. 2010;1:541-7.

Mijatovic S, Maksimovic-Ivanic D, Radovic J, Miljkovic D,Kaludjerovic GN, Sabo TJ and Trajkovic V. Aloe emodin decreases the ERKdependent anticancer activity of cisplatin. Cell Mol Life Sci. 2005;62:1275-82.

Esmaili Gouvarchin Galeh H, Delirezh N, Abtahi Froushani SM, Afzale Ahangaran NA. Calcitriol modulates the effects of the supernatants of bone-marrow–derived mesenchymal stem cells on neutrophil functions. Turkish Journal of Biology. 2014;63-72.

Winters W, Benavides R, Clouse W. Effects of aloe extracts on human normal and tumor cells in vitro. Economic botany. 1981;35(1):95-89.

Cory S. and Adams JM. The Bcl2 family: regulators of the cellular life-or-death switch. Nat Rev Cancer. 2002:2(9):647-56.

Chabner B.A. and Friedman MA. Progress against rare and not-so-rare cancers. N Engl J Med. 1992;326(8):563-5.

Lin JG, Chen GW, Li TM, Chouh ST, Tan TW, Chung JG. Aloe-Emodin induces apoptosis in T24human bladder cancer cells through the p53 dependent apoptotic pathway. The Journal of urology.2006;175(1):343-7.

HemaIswarya S, Doble M. Potential synergism of natural products in the treatment of cancer.Phytother Res. 2006;20(4):239-49.

Ait-Mohamed O, Battisti V, Joliot V, Fritsch L, Pontis J, Medjkane S, et al. Acetonic extract of Buxus sempervirens induces cell cycle arrest, apoptosis and autophagy in breast cancer cells. PloS one. 2011;6(9):e24537.

Saeed MA, Ishtiaq A, Yaqub U, Akbar SH, Amranwaheed M, Saleem N. Aloe vera: A plant of vital signinficance.Quarterly Science Vision. 2003;2:1-13.

Atul N, Kumar S, Bhattacharjee Ch, Debnath S. Cytotoxicity study of plant Aloe vera Linn.Chronicles of Young Scientists. 2012;3(3);233-5.

Kaiyuan A, Yihhuei U. Aloe emodin, an anthraquinone, in vitro inhibits proliferation and induces apoptosis in human colon carcinoma cells. Pharmaceutical Products Press. 2010;1:158–9.

Pankaj K. Therapeutic and medicinal uses of Aloe vera. A Review Pharmacology & Pharmacy. 2013;9(1-2):599-601.

Published
2019-10-31
Section
Original Article