The Antifungal Effect of Thymus Vulgaris on Isolated Candida Albicans from the Surface of Removable Orthodontic Appliances

Farkhondeh Kavianirad, Nima Bahador, Navid Naseri, Tahereh Baherimoghadam *, Roja Safaeian

Abstract


Background and Aim: Orthodontic appliances can impair the balance of oral microflora and increase the load of certain microorganisms in the oral cavity. Chlorhexidine (CHX) is the gold-standard antimicrobial agent for plaque control. However, its long-term use induces adverse effects. The present study seeks to assess the effect of the essential oil of Thymus vulgaris on isolated Candida albicans (C. albicans) from removable orthodontic appliances.

Materials and Methods: In this study, thirty-two dental students were requested to use removable orthodontic appliances for 7 days. Subsequently, the acrylic base of the appliance was checked for the isolation of C. albicans. Furthermore, the effect of Thymus Vulgaris essential oil on isolated C. albicans were evaluated using the disc diffusion method, and then its minimum inhibitory concentration (MIC) was determined.

Results: The results of this study indicated that the essential oil of Thymus vulgaris was significantly more effective than CHX in the elimination of isolated C. albicans from the surface of orthodontic appliances (P<0.05). Moreover, its MIC was calculated to be 15.6 µL/mL.

Conclusion: The essential oil of Thymus vulgaris has favorable antimicrobial activity against C. albicans greater than that of CHX.


Keywords


Removable acrylic orthodontic appliance, Thymus Vulgaris essential oil, Candida albicans, Chlorhexidine mouthwash

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References


Steinberg D, Eyal S. Initial biofilm formation of Streptococcus sobrinus on various orthodontics appliances. J Oral Rehabil. 2004;31(11):1041-5.

Littlewood SJ, Tait AG, Mandall NA, Lewis DH. The role of removable appliances in contemporary orthodontics. Br Dent J. 2001;191(6):304-6.

Houston WJB, Isaacson KG. Orthodontic Treatment with Removable Appliances. 2 nd ed. Bristol: John Wright & Sons Ltd; 1980.

Lara-Carrillo E, Montiel-Bastida NM, Sánchez-Pérez L, Alanís-Tavira J. Effect of orthodontic treatment on saliva, plaque and the levels of Streptococcus mutans and Lactobacillus. Med Oral Patol Oral Cir Bucal. 2010;15(6):e924-9.

Menzaghi N, Saletta M, Garattini G, Brambilla E, Strohmenger L. Changes in the yeast oral flora in patients in orthodontic treatment. Prev Assist Dent. 1991;17:26-30.

Anhoury P, Nathanson D, Hughes CV, Socransky S, Feres M, Chou LL. Microbial profile on metallic and ceramic bracket materials. Angle Orthod. 2002;72(4):338-43.

Batoni G, Pardini M, Giannotti A, Ota F, Giuca MR, Gabriele M, et al. Effect of removable orthodontic appliances on oral colonization by mutans streptococci in children. Eur J Oral Sci. 2001;109(6):388-92.

Chertus V, Lon R. Dental Plaque Classification, formation, and identification. International Journal of Medical Dentistry. 2013;3:139-143.

Nobbs AH, Lamont RJ, Jenkinson HF. Streptococcus adherence and colonization. Microbiol Mol Biol Rev. 2009;73(3):407-50.

Kundu R1, Tripathi AM, Jaiswal JN, Ghoshal U, Palit M, KhandujaS. Effect of fixed space maintainers and removable appliances on oral microflora in children: An in vivo study J Indian Soc Pedod Prev Dent. 2016;34(1):3-9.

Lessa FC, Enoki C, Ito IY, Faria G, Matsumoto MA, Nelson-Filho P. In-vivo evaluation of the bacterial contamination and disinfection of acrylic baseplates of removable orthodontic appliances. Am J Orthod Dentofacial Orthop. 2007;131(6):11-7.

Oshagh M, NazariDashlibrum Y, EbrahimiSaravi M, Bazargani A, Evaluation of Chlorhexidine and Zataria Multiflora Essential Oil in Removing Streptococcus Viridans and Candida from the Surface of Removable Orthodontic Appliances: a randomized clinical trial. J Mazandaran Univ Med Sci. 2014;23(110):191-99.

Garcia-Cuesta C, Sarrion-Perez MG, Bagan JV. Current treatment of oral candidiasis: a literature review. J Clin Exp Dent. 2014;6(5):576-82.

Kundu R, Tripathi AM, Jaiswal JN, Ghoshal U, Palit M, Khanduja S. Effect of fixed space maintainers and removable appliances on oral microflora in children: an in vivo study. J Indian Soc Pedod Prev Dent. 2016;34(1):3-9.

Arendorf T, Addy M. Candidal carriage and plaque distribution before, during and after removable orthodontic appliance therapy. J Clin Periodontol. 1985;12(5):360-8.

Sherman R. G., Prusinski L., Ravenel M. C., Joralmon R. A. Oral candidosis. Quintessence Int. 2002;33:521-32.

Moshrefi A. Chlorhexidine. J West Soc Periodontol Periodontal Abstr. 2002;50(1):5-9.

Bishara Se, Damon PL, Olsen ME, Jakobsen JR. Effect of applying chlorhexidine antibacterial agent on the shear bond strength of orthodontic brackets. Angle Orthodontist. 1996;66(4):313-16.

Jafari AA, Falah-Tafti A, Lotfi-Kamran MH, Zahraeii A, Kazemi A. Vinegar as a removing agent of candida albicans from acrylic resin plates. Jundishapur J Microbiol. 2012;5(2):388-92.

Ahmad A, Khan A, Akhtar F, Yousuf S, Xess I, Khan LA, Manzoor N. Fungicidal activity of thymol and carvacrol by disrupting ergosterol biosynthesis and membrane integrity against Candida. Eur J Clin Microbiol Infect Dis. 2011;30(1):41-50

Chitsazi M, Shirmohammadi A, Balayi E. Effect of herbal and chemical mouth-rinses on periodontal indices; comparison of Matrica, Persica and Chlorhexidine. J Dent. 2007;8(4):54-60.

ArbabiklatiF,SHerzaee M, Poorzamani M, Dabiri S. Inhibitory Effects of Plant Extracts Containing Thyme, Clove and Cinnamon Compared to Nystatin On Candida albicans (in vitro). J Res Dent Sci. 2012;8(4):175-9.

Chakrabarti A, Shivaprakash M, Ghosh A. Standard Operating procedures, Mycology Laboratories. Indian Council of Medical Research. 2016:5-47.

Byadarahally Raju S, Rajappa S. Isolation and identification of Candida from the oral cavity. Review Article. International Scholarly Research Network. 2011; 2011:487921.

Chandrabhan D, Hemlata R, Renu B, Pradeep V. Isolation of Dental Caries Bacteria from Dental Plaque and Effect of Tooth Pastes on Acidogenic Bacteria. Journal of Medical Microbiology. 2012;2:65-69.

Kali A, Srirangaraj S, Charles PM. A cost-effective carbohydrate fermentation test for yeast using microtiter plate. Indian J Med Microbiol. 2015 Apr-Jun; 33(2):293-5.

Yücesoy M, Guldaş NS, Yuluğ N. Disk diffusion method for fluconazole susceptibility testing of Candida albicans strains. J Chemother. 2001;13(2):161-6.

Haas AN, Pannuti CM, Andrade AK, Escobar EC, Almeida ER, Costa FO, Cortelli JR, Cortelli SC, Rode SD, Pedrazzi V, Oppermann RV. Mouthwashes for the control of supragingival biofilm and gingivitis in orthodontic patients: evidence-based recommendations for clinicians. Braz Oral Res. 2014;28(spe):1-8.

Pavarina AC, Machado AL, Giampaolo ET, Vergani CE. Effects of chemical disinfectants on the transverse strength of denture base acrylic resins. J Oral Rehabil. 2003;30(11):1085-89.

Thosar N, Basak S, Bahadure RN, RajurkarM. Antimicrobial efficacy of five essential oils against oral pathogens: An in vitro study. Eur J Dent. 2013;7:71-7.

Jafari AA, Falah Tafti A, Hoseiny SM, Kazemi A. Antifungal Effect of Zataria multiflora Essence on Experimentally ContaminatedAcryl Resin Plates with Candida albicans. Iran Red Crescent Med J. 2015;17(1):e16552.

Shahnaz H, Hifza A, Bushra K, Khan JI. Lipid studies of Cuminum Cyminum fixed oil. Pak J Bot. 2004;36(2):395-401.

Shahsavari N, Barzegar M, Sahari MA, Naghdibadi H. Antioxidant activity and chemical characterization of essential oil of Buniumpersicum. Plant Foods Hum Nutr 2008;63(4):183-8.

Sharifian M, Bolhari B, Nosrat A, Aligholi M. The effect of carvacerol on Enterococcus faecalis as an intracanal medicament-Invitro study. J Dent Med. 2009;1(22):35-40. (Persian)

Veerman EC, Nazmi K, Van’t Hof W, Bolscher JG, Den Hertog AL, Nieuw Amerongen AV. Reactive oxygen species play no role in the candidacidal activity of the salivary antimicrobial peptide histatin 5. Biochemistry Journal. 2004;381:447–52.

Pusateri CR, Monaco EA, Edgerton M. Sensitivity of Candida albicans biofilm cells grown on denture acrylic to antifungal proteins and chlorhexidine. Archives of Oral Biology. 2009; 54:588–94.

Fathilah AR, Himratul-Aznita WH, Fatheen AR, Suriani KR. The antifungal properties of chlorhexidine digluconate and cetylpyrinidiniumchloride on oral Candida. J Dent. 2012;40(7):609-15.

Ghazal ARA, Idris G, Hajeer MY, Alawer K, Cannon RD. Efficacy of removing Candida.albicans from orthodontic acrylic bases: an in vitro study. BMC Oral Health. 2019;19(1):71.




DOI: https://doi.org/10.22087/herb%20med%20j.v4i2.718

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