A Comparative Study of the Effects of the Hydroalcoholic Extract of Ziziphora Clinopodioides and Sesame on the Testicular Injury of Normal and Diabetic Mice

Amir Hossein Tozandeh, Sahar Molzemi *, Nahide Bolbol Haghighi, Farzaneh Kazemzadeh, Hamid Mohammad Sadeghi

Abstract


Background and Aim: Diabetes could play a significant role in creating metabolic damages because of generating oxidative stress. It appears that the antioxidant properties of sesame and Ziziphora clinopodioides enable them to be effective in the treatment of diabetes-induced testicular injuries.

Materials and Methods: In this study, 48 male Wistar rats with the weight range of 200 to 220gr were selected and divided into the control, the diabetic and the experimental groups of 1, 2, 3 and 4 rats. Diabetes was induced by the intraperitoneal injection of 55mg/kg of sreptozotocin. The diabetic group, the first experimental group (diabetic + 100mg sesame) the second experimental group (diabetic +150 mg sesame), the third experimental group (diabetic+ 100mg Ziziphora Clinopodioides) and the fourth experimental group (diabetic + 150 mg Ziziphora Clinopodioides) whose rats became diabetic after two months were administered sesame and Ziziphora clinopodioides extract for five weeks by intraperitoneal injection. At the beginning of the fifth week, blood samples were taken from the subjects, and biochemical factors, blood hormones as well as testicle dimensions were evaluated macroscopically and histologically.

Results: The number of sertoli and spermatid cells in the diabetic group was remarkably reduced in comparison with the control group (p<0.05). Moreover, sesame consumption significantly increased the number of sertoli cells and spermatids compared to the other experimental groups. (p<0.01). Testosterone and insulin also significantly decreased in the diabetic group compared to the control group (p<0.041), and finally sesame consumption increased them compared to the other groups (p<0.32).

Conclusion: Sesame plays a therapeutic role in improving diabetes-induced testicular injuries due to its ability to prevent and improve oxidative stresses.


Keywords


Diabetes, Sesame, Ziziphora clinopodioides, Sertoli cells, Testicular injury

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References


Maresch CC, Stute DC, Alves MG, Oliveira PF, Linn T. Diabetes-induced hyperglycemia 494 impairs male reproductive function: a systematic review, Hum. Reprod. Update. 2018;24(1):86-105.

Alves MG, Duarte AI, Santos MS, Moreira PI, Cavaco JE, Oliveira PF. Testosterone deficiency induced by progressive stages of diabetes mellitus impairs glucose metabolism and favors glycogenesis in mature rat Sertoli cells, International . journal. Biochem. Cell Biol. 2015;66:1-10.

Omolaoye TS, Skosana BT. Diabetes mellitus-induction: Effect of different streptozotocin doses on male reproductive parameters, journal Acta. Histochem. 2018;120(2):103-9.

Gandhi J, Dagur G, Warren K, Smith NL, Sheynkin YR, Zumbo A, et al. The Role of Diabetes Mellitus in Sexual and Reproductive Health: An Overview of Pathogenesis, Evaluation, and Management, Curr. Diabetes. Journal Rev. 2017;13(6):573-81.

Ghorbani A, Farahani MA, Mohammadi N. Relationship between sleepiness, physical activity,and functional outcomes in iranian patients withtype II diabetes. Jundishapur Journal Chronic Dis Care. 2014;3(1):54-60. [in Persian]

Eweda SM, Newairy ASA, Abdou HM, Gaber AS. Bisphenol A-induced oxidative damage in the hepatic and cardiac tissues of rats: The modulatory role of sesame lignans. journal Ther Med. 2020;19(1):33-44.

Tateishi N, Morita S, Yamazaki I, Okumura H, Kominami M, et al. Administration timing and duration-dependent effects of sesamin isomers on lipid metabolism in rats. Journal Chronobiol Int. 2019;13:1-17.

Ghafari H, Yasa N, Mohammadirad A, Dehghan G, Zamani MJ, Nikfar S, et al. Protection by Ziziphora clinopoides of acetic acid-induced toxic bowel inflammation through reduction of cellular lipid peroxidation and myeloperoxidase activity. Journal Human & Experimental Toxicology. 2006;25(6):325-32.

Wu Y, Wang Y, Nabi X. Protective effect of Ziziphora clinopodioides flavonoids against H2O2-induced oxidative stress in HUVEC cells .journal Biomed Pharmacother. 2019;117:109-56.

Sedighi S, Tehranipour M, Vaezi G, Hojati V, Hashemi-Moghaddam H. The effect of hydroalcoholic extract of Ziziphora clinopodioides L. on spatial memory and neuronal density of hippocampal CA1 region in rats with sporadic Alzheimer's disease. journal Avicenna Phytomed. 2019;9(4):362-73.

Allergic asthma by Ziziphora clinopodioides via upregulation of aquaporins and downregulation of IL4 and IL5. Respir. Journal Physiol Neurobiol. 2019;266:39-46.

Shahbazi Y. Chemical compositions, antioxidant and antimicrobial properties of Ziziphora clinopodioides Lam. essential oils collected from different parts of Iran. journal Food Sci Technol. 2017;54(11):3491-503.

Konyalιoglu S, Ozturk B, Meral GE. Comparison of Chemical Compositions and Antioxidant Activities of the Essential Oils of Two Ziziphora. Taxa from Anatolia. journal Pharmaceutical biology. 2006;44(2):121-6.

Sadeghi H, Mansourabadi A, Emami S, Nahvinejad M, Moogooei M. The effect of Ziziphora ethanolic extract on active pancreatic beta cells on streptozotocin induced diabetic mice,Iranian journal of Diabetes and Metabolism. 2015;14(6):373-8. [ in Persian]

Mahdavi, M. Antimicrobial effects of Ziziphora clinopodioidesextracts on Salmonella typhi Vi+ in vitro, in vivo and amp; amp; cell culture. Clinical Biochemistry. 2011;44(13):326-30. [in Persian]

Sánchez-Gutiérrez M, Martínez-Loredo E, Madrigal-Santillán EO, Betanzos-Cabrera G, Hernández-Zavala A, Mojica-Villegas M, et al. Exposure of Fluoride with Streptozotocin-Induced Diabetes Aggravates Testicular Damage and Spermatozoa Parameters in Mice. Journal Toxicol. 2019;3;2019:5269380.

Liu Y, Yang Z, Kong D, Zhang Y, Yu W, Zha W. Metformin Ameliorates Testicular Damage in Male Mice with Streptozotocin-Induced Type 1 Diabetes through the PK2/PKR Pathway. Journal Oxid Med Cell Longey. 2019;28:5681701.

Alsemeh AE, Samak MA, El-Fatah SSA. Therapeutic prospects of hydroxytyrosol on experimentally induced diabetic testicular damage: potential interplay with AMPK expression. Journal Cell Tissue Res. 2019 Dec 14. doi: 10.1007/s00441-019-03143-2.

Nna VU, Bakar ABA, Ahmad A, Umar UZ, Suleiman JB, Zakaria Z, et al. Malaysian propolis and metformin mitigate subfertility in streptozotocin-induced diabetic male rats by targeting steroidogenesis, testicular lactate transport, spermatogenesis and mating behaviour. Journal Andrology. 2019 10.1111/andr.12739

Cheng Y, Yang Z, Shi J, Yang J, Zhao J, He Y, et al. Total flavonoids of Epimedium ameliorates testicular damage in streptozotocin-induced diabetic rats by suppressing inflammation and oxidative stress. Journal Environ Toxicol. 2020;35(2):268-76.

Jiang YP, Ye RJ, Yang JM, Liu N, Zhang WJ, Ma L, et al. Protective effects of Salidroside on spermatogenesis in streptozotocin induced type-1 diabetic male mice by inhibiting oxidative stress mediated blood-testis barrier damage. Journal Chem Biol Interact. 2019;2;315:108869.

Cho EH, Lee H, Ryu OH, Choi MG, Kim SW. Sleep Disturbances and Glucoregulation in Patients with Type 2 Diabetes. Journal Korean Med Sci. 2014;29(2):243-7




DOI: https://doi.org/10.22087/herb%20med%20j.v4i4.719

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Lorestan University of Medical Sciences, Khorramabad, Iran.

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