A Comparative Study of the Effects of the Hydroalcoholic Extract of Ziziphora Clinopodioides and Sesame on the Testicular Injury of Normal and Diabetic Mice

  • Amirhosein Toozandehjani Department of Medical Radiation Engineering, Islamic Azad University, Shahroud, Iran
  • Sahar Molzemi * Department of Experimental Sciences, Islamic Azad University, Shahroud Branch, School of Medical Sciences Shahroud, Iran https://orcid.org/0000-0003-3069-5926
  • Nahide Bolbol Haghighi Department of Midwifery, School of Nursing and Midwifery, Shahroud University of Medical Sciences, Shahroud, Iran
  • Farzaneh Kazemzadeh Department of Experimental Sciences, Islamic Azad University, Shahroud Branch, School of Medical Sciences Shahroud, Iran
  • Hamid Mohammad Sadeghi Physiology Department, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iranic of Iran Army, Tehran, Iran
Keywords: Diabetes, Sesame, Ziziphora clinopodioides, Sertoli cells, Testicular injury


Background and Aim: Diabetes could play a significant role in creating metabolic damages because of generating oxidative stress. It appears that the antioxidant properties of sesame and Ziziphora clinopodioides enable them to be effective in the treatment of diabetes-induced testicular injuries.Materials and Methods: In this study, 48 male Wistar rats with the weight range of 200 to 220gr were selected and divided into the control, the diabetic and the experimental groups of 1, 2, 3 and 4 rats. Diabetes was induced by the intraperitoneal injection of 55mg/kg of sreptozotocin. The diabetic group, the first experimental group (diabetic + 100mg sesame) the second experimental group (diabetic +150 mg sesame), the third experimental group (diabetic+ 100mg Ziziphora Clinopodioides) and the fourth experimental group (diabetic + 150 mg Ziziphora Clinopodioides) whose rats became diabetic after two months were administered sesame and Ziziphora clinopodioides extract for five weeks by intraperitoneal injection. At the beginning of the fifth week, blood samples were taken from the subjects, and biochemical factors, blood hormones as well as testicle dimensions were evaluated macroscopically and histologically.Results: The number of sertoli and spermatid cells in the diabetic group was remarkably reduced in comparison with the control group (p<0.05). Moreover, sesame consumption significantly increased the number of sertoli cells and spermatids compared to the other experimental groups. (p<0.01). Testosterone and insulin also significantly decreased in the diabetic group compared to the control group (p<0.041), and finally sesame consumption increased them compared to the other groups (p<0.32).Conclusion: Sesame plays a therapeutic role in improving diabetes-induced testicular injuries due to its ability to prevent and improve oxidative stresses.


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