Evaluation of the Chemical Compounds and the Antibacterial, Antioxidant and Cytotoxic Activities of Echinophora Cinerea Boiss Essential oil

Marzieh Rashidipour, Behnam Ashrafi, Samaneh Hadavand, Fatemeh Beyranvand, Mojgan Zareivenovel *

Abstract


Background and Aim: Echinophora cinerea is a plant of the Apiaceae family, which reaches a height of 30 to 100 cm. The essential oil of Echinophora cinerea contains alkaloids and flavonoids. Phenolic Compounds are one of the best source of natural antioxidants. The goal of this study was to evaluate chemical compounds, antibacterial, and antioxidant activity of Echinophora Cinerea Boiss´s essential oil.

Materials and Methods: Preparation and identification of chemical compounds of essential oil, was performed by hydrodistillation method and gas chromatography coupling with a mass spectrometer (GC/MS). Antioxidant capacity of essential oils was determined by DPPH and was compared with standard antioxidants (BHT). Antibacterial test was carried out by Broth Micro Dilution, Disk Diffusion methods, Minimum Inhibitory Concentration (MIC), and Minimum Bacteriocidal Concentration (MBC) methods. These oils after 24 and 48 h determined and compared to control of antibiotic. Plant antibacterial activity of essential oil on Staphylococcus aureus, Listeria monocytogenes, Pseudomonas aeruginosa, Escherichia coli and MRSA were assessed.

Results: The main constituents of essential oil were combined with plant α-phellandrene (32/09), limonene (16/28), P-cymene (10.75), α-pinene (9.79), carvacrol (3.79), and β-Myrcene (2.65). For oil of Echinophora cinerea is 0.74 (mg / ml) but this parameter is set to BHT 50.63 (μg / ml), respectively. Strong antibacterial effects of essential oils were observed on Staphylococcus aureus (MIC = 0.16 μg/ml and MBC = 0.63 μg/ml. The effect on MRSA-resistant staphylococcus and E. coli was weaker than S. aureus. Pseudomonas aeruginosa was resistant to this essential oil.

Conclusion: The antibacterial and antioxidant properties of plant essential oil can be used in the food, pharmaceuticals, cosmetics and health industry.


Keywords


Echinophora cinerea, antioxidant, antibacterial, GC-MS, DPPH

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References


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DOI: https://doi.org/10.22087/herb%20med%20j.v5i1.728

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