Comparison between MPO, CD177 and NF-κB Gene Expression of Septic Rats Treated with Mentha longifolia Essential Oils
Abstract
Background and Aim: Incidences range of sepsis-associated liver dysfunction and lung injury are observed in patients with sepsis. Mentha longifolia as a native Iranian plant have many properties such as antimicrobial and antioxidant activity. In this regard, the anti-inflammatory effect of Mentha longifolia essential oil (E.O) on liver and lung injury induced by sepsis was investigated.Materials and Methods: Gene expression of three genes (MPO, CD177 and NF-κB) as inflammatory factors was evaluated by real-time PCR in four experimental groups (Laparotomy (LAP), cecal ligation and puncture (CLP) and essential oil groups at 50 and 100 mg/kg body weight doses). The treatment was carried out orally for two weeks before CLP surgery. 24 hours after CLP, samples were immediately transferred to -80ºC to maintain for further experiments.Results: Expression of genes increased in the CLP group as compared to the LAP group. Treatments of rats with essential oil have been effective in decreasing genes expression levels in two dosages at two tissues.Conclusion: Our results demonstrated that M. longifolia essential oil treatment could protect against sepsis-induced liver and lung injury by reducing inflammatory factor.References
Xu C, Liu Q, Zhang Q, Jiang Z-Y, Gu A. Urinary enterolactone associated with liver enzyme levels in US adults: National Health and Nutrition Examination Survey (NHANES). British Journal of Nutrition. 2015;114(1):91-7.
Yan J, Li S, Li S. The role of the liver in sepsis. International reviews of immunology. 2014;33(6):498-510.
Liu A, Wang W, Fang H, Yang Y, Jiang X, Liu S, et al. Baicalein protects against polymicrobial sepsis-induced liver injury via inhibition of inflammation and apoptosis in mice. European journal of pharmacology. 2015;748:45-53.
Seymour CW, Iwashyna TJ, Cooke CR, Hough CL, Martin GS. Marital status and the epidemiology and outcomes of sepsis. Chest. 2010;137(6):1289-96.
Cao T-h, Jin S-g, Fei D-s, Kang K, Jiang L, Lian Z-y, et al. Artesunate protects against sepsis-induced lung injury via heme oxygenase-1 modulation. Inflammation. 2016;39(2):651-62.
Eichacker PQ, Parent C, Kalil A, Esposito C, Cui X, Banks SM, et al. Risk and the efficacy of antiinflammatory agents: retrospective and confirmatory studies of sepsis. American journal of respiratory and critical care medicine. 2002;166(9):1197-205.
Fattahi F, Ward PA. Understanding immunosuppression after sepsis. Immunity. 2017;47(1):3-5.
Selvaraj V, Nepal N, Rogers S, Manne ND, Arvapalli R, Rice KM, et al. Inhibition of MAP kinase/NF-kB mediated signaling and attenuation of lipopolysaccharide induced severe sepsis by cerium oxide nanoparticles. Biomaterials. 2015;59:160-71.
Klebanoff SJ. Myeloperoxidase: friend and foe. Journal of leukocyte biology. 2005;77(5):598-625.
Khowailed A, Younan SM, Ashour H, Kamel AE, Sharawy N. Effects of ghrelin on sepsis-induced acute kidney injury: one step forward. Clinical and experimental nephrology. 2015;19(3):419-26.
Demaret J, Venet F, Plassais J, Cazalis M-A, Vallin H, Friggeri A, et al. Identification of CD177 as the most dysregulated parameter in a microarray study of purified neutrophils from septic shock patients. Immunology letters. 2016;178:122-30.
Bai M, Grieshaber-Bouyer R, Wang J, Schmider AB, Wilson ZS, Zeng L, et al. CD177 modulates human neutrophil migration through activation-mediated integrin and chemoreceptor regulation. Blood. 2017;130(19):2092-100.
Edziri H, Marzouk B, Mabrouk H, Garreb M, Douki W, Mahjoub A, et al. Phytochemical screening, butyrylcholinesterase inhibitory activity and anti-inflammatory effect of some Tunisian medicinal plants. South African Journal of Botany. 2018;114:84-8.
Gulluce M, Sahin F, Sokmen M, Ozer H, Daferera D, Sokmen A, et al. Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp. longifolia. Food chemistry. 2007;103(4):1449-56.
Bellassoued K, Hsouna AB, Athmouni K, van Pelt J, Ayadi FM, Rebai T, et al. Protective effects of Mentha piperita L. leaf essential oil against CCl 4 induced hepatic oxidative damage and renal failure in rats. Lipids in health and disease. 2018;17(1):9.
Tardugno R, Pellati F, Iseppi R, Bondi M, Bruzzesi G, Benvenuti S. Phytochemical composition and in vitro screening of the antimicrobial activity of essential oils on oral pathogenic bacteria. Natural product research. 2018;32(5):544-51.
Rasooli A, Ghafari E, Saeidi H, Miri S. Expression changes of CD177 and MPO as novel biomarkers in lung tissue of CLP model rats. Turkish journal of medical sciences. 2018;48(6):1321-7.
Dadkhah A, Fatemi F, Rasooli A, Mohammadi Malayeri MR, Torabi F. Assessing the effect of Mentha longifolia essential oils on COX-2 expression in animal model of sepsis induced by caecal ligation and puncture. Pharmaceutical Biology. 2018;56(1):495-504.
Brown K, Brain S, Pearson J, Edgeworth J, Lewis S, Treacher D. Neutrophils in development of multiple organ failure in sepsis. The Lancet. 2006;368(9530):157-69.
Angus DC, Van der Poll T. Severe sepsis and septic shock. New England Journal of Medicine. 2013;369(9):840-51.
Amulic B, Cazalet C, Hayes GL, Metzler KD, Zychlinsky A. Neutrophil function: from mechanisms to disease. Annual review of immunology. 2012;30:459-89.
Lill M, Taba P, Kõks S, Soomets U, Lutsar I, Schalkwyk LC, et al. Peripheral blood RNA gene expression profiling in patients with bacterial meningitis. Frontiers in neuroscience. 2013;7:33.
Kovach MA, Standiford TJ. The function of neutrophils in sepsis. Current opinion in infectious diseases. 2012;25(3):321-7.
Ding J, Song D, Ye X, Liu SF. A pivotal role of endothelial-specific NF-κB signaling in the pathogenesis of septic shock and septic vascular dysfunction. The Journal of Immunology. 2009;183(6):4031-8.
Amirghofran Z, Hashemzadeh R, Javidnia K, Golmoghaddam H, Esmaeilbeig A. In vitro immunomodulatory effects of extracts from three plants of the Labiatae family and isolation of the active compound (s). Journal of immunotoxicology. 2011;8(4):265-73.
Amirghofran Z, Malek-hosseini S, Gholmoghaddam H, Kalalinia F. Inhibition of tumor cells growth and stimulation of lymphocytes by Euphorbia species. Immunopharmacology and immunotoxicology. 2011;33(1):34-42.
Eissa TF, Gonzalez-Burgos E, Carretero M, Gómez-Serranillos M. Phenolic content, antioxidant and astroprotective response to oxidative stress of ethanolic extracts of Mentha longifolia from Sinai. Natural product communications. 2014;9(10):1479-82.
Mokaberinejad R, Zafarghandi N, Bioos S, Dabaghian FH, Naseri M, Kamalinejad M, et al. Mentha longifolia syrup in secondary amenorrhea: a double-blind, placebo-controlled, randomized trials. DARU Journal of Pharmaceutical Sciences. 2012;20(1):97.
Shi M, Zhao C, Ge X, Yang H, Ge L, Zhu G, et al. Berberine Prevents Cognitive Disorders Induced by Sepsis by Regulating the Inflammatory Cytokines, Oxidative Stress and Neuronal Apoptosis in Rat Brain. Neuropsychiatry. 2018;8(1):17-26.
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