Survey and In Vitro Investigation of the Plants Used by Traditional Healers in the Treatment of Diabetes in Albay Province, the Philippines

Dennis B. Gogola *, Maria Luisa U. Tango

Abstract


Abstract

Background and Aim: Diabetes is a global lifestyle disease which affects the health and economy of the affected population. It causes debilitating complications, which requires expensive medications, but produces side effects. With this, the study aimed to identify plants used in the treatment of diabetes by traditional healers in Albay province, Philippines and evaluated their inhibitory activity against alpha-glucosidase and DPPH free radicals, and determined presence of essential phytochemicals.

Materials and Methods: The anti-diabetic activity of the plant extracts determined by α-glucosidase inhibition assay. Free radical scavenging activity of the plants evaluated using DPPH (1, 1-Diphenyl-2 -picrylhydrazyl). Also, the determination of total phenolics, tannins, and flavonoids were performed using conventional methods.

Results: The study found nine plants used, namely A. muricata, B. balsamifera, M. oleifera, C. citratus, C. nucifera, C. longa, M. charantia, A. esculentus, and C. microcarpa. The inhibitory activity against α-glucosidase actively exhibited by B. balsamifera leaf and C. nucifera root extracts with IC50 values of 4.97 and 13.36 μg/ml, respectively. Further, the plant extracts showed free radical scavenging capacity that ranged from 38.88 to 90.36% at the concentration of 100 μg/mL. The B. balsamifera exhibited the highest radical scavenging activity, with 90.36%, followed by C. nucifera with 88.57 %. Also, the total phenolic, flavonoid, and tannin contents found higher in C. nucifera with 609.5, 98.3, and 1,577.70mg GAE/g dw, respectively. While, B. balsamifera with 276.4mg GAE/gm phenolic, 611.1mg GAE/gm tannin and 63.3mg GAE/gm dw flavonoid contents, respectively.

Conclusion: The results suggest that only two among the nine plants displayed inhibitory activity against α-glucosidase, and also exhibited antioxidant capacity and they contained essential bioactive compounds. Hence, the results provide scientific support to the potential use of these plants in folklore medicine for diabetes treatment.

Keywords:

Keywords


Diabetes,; Alpha-glucosidase inhibition; Antioxidant; C. nucifera; B. balsamifera

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DOI: https://doi.org/10.22087/herb%20med%20j.v4i4.761

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