Effectiveness of household Plants in Phytochemical constituents, antimicrobial activity, and antioxidants with different solvents
AbstractThe phytochemical profile and antimicrobial activity of leaf extracts obtained from Acalypha indica, Azadirachta indica, Lawsonia inermis and Murraya koneigii were studied. In the present work, plant extracts were prepared using ethanol, methanol, and water as solvents. Crude and soxhlet method were used for extraction process. Qualitative phytochemical analysis was done to reveal the presence of alkaloids, flavonoids, tannins, phenols, saponins, steroids, and anthocyanins. Quantitative analysis were done for the estimation of phenolic content and vitamin C. Soxhlet methanolic leaf extracts of Acalypha indica (4.80mg/ml) showed high level of phenolic content and Vitamin C content was found to be high in methanolic crude and soxhlet extract of Murraya koenigii (3.7mg/ml and 2.4mg/ml). Antimicrobial assay was carried out by agar disk diffusion method against seven bacterial strains. Gram positive organisms such as Staphylococcus aureus, Bacillus subtilis and gram-negative organisms like Klebsiella pneumonia, Proteus mirabilis, Pseudomonas aeruginosa, Enterobacter faecalis and Escherichia coli were used. The antimicrobial activities of each extract were assessed by measuring the diameter zone of inhibition. The plant extracts tested were highly effective against Proteus mirabilis and Enterobacter fecalis. Compared to aqueous extracts, methanolic and ethanolic extracts showed more antimicrobial activity. Methanolic extract of Lawsonia inermis showed higher zone of inhibition compare to other extracts.
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