Lorestan University of Medical Sciences Herbal Medicines Journal 2538-2144 8 3 2023 05 01 Anti-Inflammatory Properties of Chitosan Hydrogels Containing Satureja Khuzestanica Jamzad Essential Oil in an Animal Model 133 143 220975 10.22087/hmj.v8i3.1017 EN Marzieh Rashidipour Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran. Environmental Health Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran. Esmaeel Babaeenezhad Nutritional Health Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran. Samaneh Hadavand Environmental Health Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Marziesana Elahian Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran. Asma Alekasir Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran. Mojtaba Khaksarian Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran. Journal Article 2025 05 17 Background and Aim: Nanoparticle shells protect bioactive compounds against the environmental conditions of light, heat, and oxygen, and are also used as carriers for targeted delivery. In the present study, the anti-inflammatory effects of a chitosan-based nanogel containing Satureja Khuzestanica essential oil (SKE:NP) were studied and compared with Satureja Khuzestanica essential oil (SKE).<br />Materials and Methods: Nanoparticles were prepared using the ionic gelation method. The anti-inflammatory<br />effects of the chitosan-based hydrogel containing SKE, using the paw inflammatory model induced with 2.5%formalin at different times (0, 1, 2, 3, 4, and 24 h after subcutaneous injection), were evaluated.<br />Results: Nanoparticles with a particle size of 552.8 nm, a surface charge of 24.3 mV, and an encapsulation efficiency of 30.74% were synthesized. The results of this study showed that SKE:NP (50 mg/kg) could reduce inflammation at all times (0, 1, 2, 3, 4, and 24 h after subcutaneous injection). The hind paw was compared with diclofenac sodium at times (0, 1, 2, 3, 4 h after subcutaneous injection), and a significant decrease in paw inflammation was observed. The lowest amount was observed in the group receiving nanogel 50 mg (0.208 g/cm3). In contrast, the amount of paw swelling in the group receiving SKE at a dose of 100 mg/kg two hours after the applied treatment was 0.441 g/cm3 (P<0.0001).<br />Conclusion: A comparison of the results showed that SKE:NP had more anti-inflammatory effects than SKE.Explanation of the exact mechanism of this anti-inflammatory effect requires a separate investigation. https://hmj.lums.ac.ir/article_220975_7f9e1140b32064d76db928842bfdb266.pdf