Lorestan University of Medical Sciences Herbal Medicines Journal 2538-2144 2 3 2017 05 01 The Effect of Rosmarinus officinalis L Extract on the Inhibition of High Glucose-Induced Neurotoxicity in PC12 Cells: an In Vitro Model of Diabetic Neuropathy 114 121 221547 10.22087/hmj.v0i0.636 EN Marzieh Rashidipour Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Zahra Hajializadeh Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran Saeed Esmaeili-Mahani Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran Ayat Kaeidi 4 Physiology-pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran 5 Department of Physiology and Pharmacology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran Journal Article 2025 05 24 Background and Aim: Diabetes mellitus and continued hyperglycemic condition prompt serious diabetic complications such as diabetic neuropathy. Numerous studies have shown the involvement of oxidative stress and high glucose-induced cell death in the progress of diabetic neuropathy. Rosmarinus officinalis L has been suggested in the literature as an anti-diabetic herbal medicine that contains potent antioxidant components. Therefore, the neuroprotective effect of Rosmarinus officinalis L (RE) extract was investigated in glucose-induced neurotoxicity via pheochromocytoma )PC12( cells as an appropriate in vitro model of diabetic neuropathy.Materials and Methods: Cell viability was determined using MTT assay. Cleave caspase-3 and Bax: Bcl2 ratio, as biochemical parameters of cellular apoptosis, were measured by western blotting analysis.Results: Our data showed that a 4-fold elevation in the medium glucose significantly reduced cell viability (P < 0.01) and increased caspase-3 activation (P < 0.01) as well as Bax: Bcl2 ratio (P < 0.05) in PC12 cells after 24 h. Incubation of high glucose medium cells with 60μg/ml RE extract decreased high glucose-induced cell toxicity and prevented caspase-3 activation and Bax: Bcl2 ratio.Conclusion: It could be concluded that RE extract is effective in the protection against hyperglycemia-induced cellular toxicity. This could be relevant to the prevention of apoptosis. Moreover, the results suggest that RE has the therapeutic potential to attenuate diabetes complications such as that neuropathy. https://hmj.lums.ac.ir/article_221547_244ecf7a2b387a51ea4f0532d9464677.pdf