Lorestan University of Medical Sciences Herbal Medicines Journal 2538-2144 2 3 2017 05 01 Antileishmanial Activity of Myrtle Methanolic Extract against Leishmaniamajor: an In Vitro Study 122 125 221549 10.22087/hmj.v0i0.637 EN Hossein Mahmoudvand Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Hamed Salehi Lalehmarzi Bogomolets National Medical University, Kyiv, Ukraine Shirzad Fallahi Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Maryam Moslehi Baharanchi 3 Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran Pardis Bayat 3 Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran Sareh Jahanbakhsh Bam University of Medical Sciences, Bam, Iran Journal Article 2025 05 24 Background and Aim: In this study we assessed the in vitro antileishmanial activity of myrtle (Myrtus communis L.) methanolic extract against Leishmania major.Materials and Methods: The in vitro antileishmanial effects of myrtle methanolic extract against L. major promastigote and amastigotes were determined by colorimetric cell viability (MTT) assay and macrophage model, respectively. The IC50 values were also calculated by probit test in SPSS software.Results:The obtained results showed that myrtle extract was significantly inhibited promastigote growth of L. major based on a dose and time dependent manner. The measured IC50 values for myrtle methanolic extract and MA as control drug against promastigote forms of L. major were 23.6 µg/mL and 88.3 µg/mL, respectively. The obtained IC50 values were 13.8 https://hmj.lums.ac.ir/article_221549_8c1327efea64c02e376c026a8a696b38.pdf