Lorestan University of Medical Sciences Herbal Medicines Journal 2538-2144 1 1 2016 05 01 Oleuropein Reduces Cisplatin-Induced Nephrotoxicity in Human Embryonic Renal Epithelial “GP-293” Cells 18 23 221574 EN Ayat Kaeidi Department of Physiology and Pharmacology, Rafsanjan University of Medical Sciences, Rafsanjan, Iran Maryam Rezaei Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Marzieh Rashidipour Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran Journal Article 2025 05 24 Background and Aim:The protective effect of oleuropein,a chief polyphenol compound in olive tree, on cisplatin-induced nephrotoxicity in human cultured renal tubular cells, GP-293, was investigated.Materials and Methods:Cell viability was determined by the MTT assay. Cleaved caspase-3 and Bax: Bcl2 ratioas biochemical parameters of cellular apoptosis, was assessed using western blot analysis.Results:Our data showed that cisplatin in the dose of 55µg/ml significantly can reduced cell viability, increased caspase-3 activation and Bax: Bcl2 ratio in GP-293 cells after 24hr. Incubation of the cisplatin treated cells, with 20μg/ml oleuropein decreased the cisplatin-induced cell toxicity, as well as prevented caspase-3 activation and reduced Bax: Bcl2 elevation ratio.Conclusions: Oleuropein protects against cisplatin-induced cellular toxicity. This could be associated with the prevention of cellular apoptosis https://hmj.lums.ac.ir/article_221574_c68cce0f709db5a976ee5324bbdaec98.pdf