Lorestan University of Medical SciencesHerbal Medicines Journal2538-21447320220501An Investigation of the Cytotoxicity Effect of the Ziziphus jujuba L., Ribes khorasanicum, Crocus sativus Petal, and Centella asiatica Extracts on the NIH/3T3 Cell Line778422109410.22087/hmj.v7i3.986ENSamiraShahrakiDepartment of Physiology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IranFatemehShahbeikiStudent Research Committee, Mashhad University of Medical Sciences, Mashhad, IranRezaMohebbatiDepartment of Physiology, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, IranJournal Article20250518Background and Aim: Medicinal plants are used for treating different diseases in humans. In the present study, we investigated the cytotoxicity effect of the hydroalcoholic extracts of Ziziphus jujuba L., Ribes khorasanicum, Crocus sativus petal, and Centella asiatica on the cell viability and morphology of embryonic fibroblast NIH/3T3 cell line in mice. Materials and Methods: In this analytic cross-sectional study, we prepared the hydroalcoholic extracts of Ziziphus jujuba L., Ribes khorasanicum, Crocus sativus petal, and Centella asiatica to treat NIH/3T3 cell line using various concentrations of these extracts (78.1, 156.5, 312.5, 625, 1250, 2500, and 5000 μg/ml). Subsequently, we investigated cell viability with MTT assay and morphological changes using an inverted light microscope after 24h and 48 h. One-way analysis of variance was performed to compare the groups using SPSS software. Results: The results of this study indicated that the hydroalcoholic extracts of the Ziziphus jujuba L. and Ribes khorasanicum could induce less toxicity and morphological change in the NIH/3T3 cells (P<0.001). NIH/3T3 cells exposed to several concentrations of Crocus sativus petal and Centella asiatica hydroalcoholic extracts reduced cell viability and exhibited morphological changes (P<0.01) such as decreased number of live cells, and lost their spindle-like shape compared with the control group in a dose-dependent manner. Conclusion: Our findings showed that none of the Crocus sativus petal, Centella asiatica, Ziziphus jujuba L. and Ribes khorasanicum extracts had any cytotoxicity effects on NIH/3T3 cell line due to high IC50, although Ziziphus jujuba L. and Ribes khorasanicum exhibited greater degrees of safety than Crocus sativus petal and Centella asiatica. https://hmj.lums.ac.ir/article_221094_963fe449f8ce795b2a07548f0b8f5484.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21447320220501In vitro Screening of Glutathione- S -Transferase Inhibition by Different Extracts of Verbascum sinuatum859022109510.22087/hmj.v7i3.901ENLubnaAbdallahDepartment of Biology and Biotechnology, An-Najah National University, Nablus, PalestineAseelAbu HardanDepartment of Biology and Biotechnology, An-Najah National University, Nablus, PalestineJournal Article20250518Background and Aim: The concern toward using herbs as enzyme inhibitors such as glutathione-s-transferases (GSTs) may result in the improvement of proliferative diseases. Moreover, it may overcome the problem of multidrug resistance tumors. Based on that, this experiment was conducted to detect the impact of using aqueous, ethanol and methanol extracts from Verbascum sinuatum L. (Scrophulariaceae) that grow wild in Palestine as enzymatic inhibitors. Materials and Methods: The impacts of these three extracts at four studied concentrations (250, 500, 750 and 1000 µg/mL) on the activity of purified hepatic glutathione-s-transferases were estimated spectrophotometrically by using-chloro-2,4-dinitro-benzene (CDNB) as substrate. Results: The results of the present research indicated that all the three prepared extracts of V. sinuatum could inhibit the activity of GSTs at all examined concentrations. Moreover, according to the results, the alcoholic extracts from the studied plant species were more influential in the activity of GSTs than the aqueous ones. However, minor inhibitory variations were observed between methanol and ethanol extracts. It is notable that all the studied extracts manifested inhibitory effects in a dose-dependent manner. Conclusion: The results of this study emphasized the possibility of utilizing V. sinuatum extracts in pharmaceutical industry of new medications to fight drug resistant tumors in general and GST-induced tumors in particular. https://hmj.lums.ac.ir/article_221095_d2968d41b241c6225066b883b3ca0af4.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21447320220501Effects of Zataria Multiflora, Mentha Longifolia, and Origanum Vulgare Plant Essential Oils on the Inhibition of Candida Albicans10310922109910.22087/hmj.v7i3.1023ENRoya MalekHossriniDepartment of Microbiology, Qom Branch, Islamic Azad University, Qom, IranFarbodTeymouriDepartment of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, IranPegahShakibRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranMohammadArjomandzadeganDepartment of Microbiology and Immunology, School of Medicine, Infectious Diseases Research Center, Arak University of Medical
Sciences, Arak, IranMohammad RezaZolfaghariDepartment of Microbiology, Qom Branch, Islamic Azad University, Qom, IranJournal Article20250518Background and Aim: In recent years, yeast, particularly Candida species, has been the most common fungus separated from human infections. In this research, effects of essential oils of some plants on the clinical strains of Candida albicans were examined. Materials and Methods: Forty clinical strains of Candida were obtained from the Infectious Diseases Research Center of Arak University of Medical Sciences. After preparing the essential oils of Zataria multiflora, Mentha longifolia, and Origanum vulgare, the minimum inhibitory concentration (MIC) of the essential oils against Candida albicans strains was determined. the Morphological changes at different times were investigated using the negative staining method and a transmission electron microscope (TEM). Results: The results of the disc diffusion test indicated that the highest resistance rates in 0.625 mg/ml of O. vulgare, M. longifolia, and Z. multiflora essential oils were 31(77.5%), 15(37.5%), and 13(32.5%), respectively. The lowest MIC was related to Z. Multiflora essential oil (0.625 mg/ml). After treatment with the essential oils, the yeasts immediately decreased at zero hour. This decrease became more noticeable with the passage of time and reached the minimum number after 24 hours. Moreover, electron microscope images showed changes in the morphology of the yeasts. Conclusion: Zataria multiflora, Mentha longifolia, and Origanum vulgare essential oils, particularly Zataria multiflora, had antimicrobial effects against Candida albicans that were isolated to be studied. Thus, Zataria multiflora essential oil can be used as an anti-candida agent in the preparation of antifungal compounds https://hmj.lums.ac.ir/article_221099_5c0ab7774dcaae2d493bfdb75fbe6997.pdf