Lorestan University of Medical SciencesHerbal Medicines Journal2538-21447220220501The Coagulant Effect of the Medicago sativa L. Hydroalcoholic Extract: An in vivo Study on Mice455222114910.22087/hmj.v7i2.954ENZahra SadatMashkaniDepartment of Biology, Hakim Sabzevari University, Sabzevar, IranJafarVatandoostDepartment of Biology, Hakim Sabzevari University, Sabzevar, IranToktamHajjarDepartment of Biology, Hakim Sabzevari University, Sabzevar, IranBehnamMahdaviDepartment of Chemistry, Hakim Sabzevari University, IranJournal Article20250519Background and Aim: Herbal medicines are used in the treatment of many diseases, including blood-related disorders. Mediacgo sativa, which is used in traditional medicine to stop bleeding, contains several secondary compounds, is assumed to be effective in primary and secondary blood coagulation. The aim of this study was to evaluate the effect of M. sativa extract on blood coagulation system in vivo. Materials and Methods: Twenty-four male mice were randomly divided into 3 groups (n = 8) as follows: negative control (receiving 0.3 ml of distilled water), positive control (receiving 0.3 ml of tranexamic acid, 1200 mg/kg/day) and the treatment group (receiving 0.3 ml of the hydroalcoholic extract of M. sativa, 300 mg/kg/day). On the 13th day of treatment, collection of blood samples from the tail tip for bleeding time (BT), clotting time (CT), and number of platelets was carried out. On the 14th day, the blood samples were collected from the heart and prothrombin time (PT), activating the partial thromboplastin time (aPTT) as indicators of coagulation rate that were determined. The GC-MS was also used to determine extract compounds. Results: The results indicated that M. sativa extract had a significant prolonged effect on the aPTT and PT. Furthermore, this extract increased platelet count, though it significantly reduced coagulation time in BT and CT. It was concluded that M. sativa extract could have beneficial effects on the initial blood homeostasis. Conclusion: Given the highly beneficial effect on coagulation and then tranexamic acid, this extract can be recommended as a potential homeostatic remedy after further clinical evaluations. https://hmj.lums.ac.ir/article_221149_0bf83abdb2dee8f1e138316d4d29ed7a.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21447220220501A Comparison of Antibacterial Effects of Licorice Root Ethanolic Extract, Chlorhexidine, and Doxycycline on Fusobacterium nucleatum: An in Vitro Study536022115010.22087/hmj.v7i2.999ENMohammad RezaTabatabaeianSchool of Dentistry, Isfahan (khorasgan) Branch, Islamic Azad University, Isfahan, IranVahidEsfahanianDepartment of Periodontology, Faculty of Dentistry, Isfahan (khorasgan) Branch, Islamic Azad University, Isfahan, IranArezooTahmourespourDepartment of Basic Medical Sciences, Faculty of Dentistry, Isfahan (khorasgan) Branch, Islamic Azad University, Isfahan, IranJournal Article20250519Background and Aim: Antibiotics and mouthwashes which are used to prevent and treat periodontal diseases have side effects such as antibiotic resistance, mouth burning, and xerostomia. With the advancement of technology, plants have been considered as alternative antibacterial agents. Licorice plant with different species has been used in traditional medicine to treat gastritis and respiratory diseases. Considering the properties of licorice in traditional medicine, this study aimed to investigate the antibacterial properties of licorice root extract with different concentrations compared with Chlorhexidine mouthwash and Doxycycline antibiotic on Fusobacterium nucleatum in vitro. Materials and Methods: After the preparation of 0.5 McFarland bacterial suspension, the plant and its ethanolic extract, and six extract dilutions were also prepared. Agar disk diffusion and broth microdilution tests were carried out against Fusobacterium nucleatum ATCC 2558. The antibacterial effect of Chlorhexidine mouthwash 0.2% and Doxycycline 100 mg antibiotic were also determined to be compared with licorice extract. The data were analyzed using Mann-Whitney and Kruskal-Wallis statistical tests in spss26 software at a significance level of 5%. Results: All the six concentrations had significant antibacterial effects compared with each other, chlorhexidine and doxycycline (P-value<0.05). The inhibitory concentrations of extract, MIC50, MIC70, and, MIC90 were related to 12/5, 50, and 200 mg/ml, respectively. The inhibitory percentages of chlorhexidine and doxycycline were 67.6% and 88.7%, respectively. Conclusion: Licorice ethanolic extract exhibited an excellent antimicrobial effect (MIC=6.25mg/ml), so that in concentrations higher than 25, a greater antimicrobial effect was observed than in chlorhexidine (P<0.05). https://hmj.lums.ac.ir/article_221150_c1bfb6299db5af0feb8b86f776381709.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21447220220501An Evaluation of the Antibacterial Properties of Oliveria decumbents against Bacteria Isolated from Patients with Respiratory Infections687422115310.22087/hmj.v7i2.976ENReyhaneRouhi1Research Center for Noncommunicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran
2Department of Bacteriology & Virology, School of Medicine, Jahrom University of Medical Sciences, Jahrom, IranNaserHatamiStudent Research Committee, Jahrom University of Medical Sciences, Jahrom, IranHelmaHoorangStudent Research Committee, Kashan University of Medical Sciences, Kashan, IranFarhadMoradi5Department of Bacteriology & Virology, School of medicine, Shiraz University of Medical Sciences, Shiraz, Iran
6Student Research Committee, Shiraz University of Medical Sciences, Shiraz, IranJournal Article20250519Background and Aim: Medicinal plants have long been used as an alternative or complement to the treatment and prevention of respiratory tract infections. The present study attempts to investigate the antibacterial effects of Oliveria decumbents on bacterial infections isolated from a hospitalized patient with respiratory disorders. Materials and Methods: Twenty-five respiratory infection samples were collected from fifty-one patients that were hospitalized in the intensive care unit of the medical centers of Jahrom, Iran. Plant materials were obtained from the natural environment areas around Jahrom, and hydro-alcoholic extraction was prepared through the percolation method. The antimicrobial impacts of the plant extract were evaluated by the disc diffusion method against clinical and standard strains, and the results were compared with common antibiotics. Moreover, the minimum inhibitory concentration (MIC) of the herbal extracts was assessed by the broth macro dilution method. Results: From the 51 clinical samples, S. aureus (11 cases, 21.5%), S. pneumoniae (8 cases, 15.6%), and P. aeruginosa (6 cases, 11.7%) were isolated. Furthermore, this herbal extract showed antibacterial activity by inhibiting the growth of three strains of S. aureus and one strain of S. pneumoniae in comparison to Amoxicillin and Amoxicillin-Clavulanate. Nevertheless, this extract did not affect P. aeruginosa clinical isolates. The MIC activity of the herbal extract for S. aureus with a concentration of 25 µg/ml, for S. pneumoniae with 50 µg/ml, and for P. aeruginosa with 200 µg/ml was observed. Conclusion: Hydro-alcoholic extract of O. decumbens showed antibacterial effects on different bacterial strains that were isolated from respiratory infections. https://hmj.lums.ac.ir/article_221153_e6d9046443d191a7bd91bd20539f8014.pdf