Lorestan University of Medical SciencesHerbal Medicines Journal2538-21445420200501The Role of Fennel Seed Extract on the Expression Pattern of Dysadherin, E-Cadherin and Ki67 in Metastatic Lung Cancer in BALB/C Female Mice13514422125210.22087/herb%20med%20j.v5i4.832ENAlirezaMehralikhaniDepartment of Biochemistry, Islamic Azad University, Tehran North Branch, Tehran, IranMonirehMovahediDepartment of Biochemistry, Islamic Azad University, Tehran North Branch, Tehran, IranMohaddesehLarypoorDepartment of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, IranFereshtehGolabCellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, IranJournal Article20250520Background and Aim: Some herbs have anti-cancer properties such as the anticancer potential of fennel methanolic extract. Metastasis is an undesirable phenomenon that occurs in most cancers. Inhibition of metastatic proteins by fennel extract can increase survival chance. Materials and Methods: Thirty-five adult BALB / C mice were divided into five groups. Four groups were infected with 4T1 cells and one group was considered as healthy control. After one week, three groups were treated with an aqueous extract of fennel seed. The mice were anesthetized after two weeks, and the lung tissues were removed. The expression of Ki67 and dysadherin was evaluated using the qRT-PCR. Moreover, we examined E-cadherin protein expression using the IHC method. Results: Ki67 expression decreased at 100 mg/kg dose (Kruskal-Wallis test: P<0.05) but revealed a remarkable increase at a 200 mg/kg dose (p<0.05). Dysadherin expression significantly decreased at all the three doses (ANOVA test: p <0.05). E-cadherin showed non-significant expression (ANOVA test P>0.05). Conclusion: A 100 mg/kg dose could noticeably decrease the expression of dysadherin and Ki67. The dose of 200 mg/kg can cause a bad prognosis. https://hmj.lums.ac.ir/article_221252_ba2a93049f0ba3f8dd0777ddaabe7080.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21445420200501Subchronic Toxicity of the Ethanolic Extract of Lecaniodiscus cupanioides on Albino Wistar Mice (Mus musculus)14515222125310.22087/herb%20med%20j.v5i4ENOloyedeAMDepartment of Cell Biology and Genetics, University of Lagos, Akoka, Lagos-NigeriaOttuBDepartment of Cell Biology and Genetics, University of Lagos, Akoka, Lagos-NigeriaOgunsanwoADepartment of Cell Biology and Genetics, University of Lagos, Akoka, Lagos-NigeriaAromolaranCDepartment of Cell Biology and Genetics, University of Lagos, Akoka, Lagos-NigeriaBolarinwaKDistance Learning Institute, University of Lagos, NigeriaOgidiEDepartment of Cell Biology and Genetics, University of Lagos, Akoka, Lagos-NigeriaOkaforEDepartment of Cell Biology and Genetics, University of Lagos, Akoka, Lagos-NigeriaSobiyeSDepartment of Cell Biology and Genetics, University of Lagos, Akoka, Lagos-NigeriaJournal Article20250520Background and Aim: Lecaniodiscus cupanioides has been used in traditional therapies in Nigeria for the management of several ailments. This study investigated its subchronic toxicity. Materials and Methods: 30 mice (20–25g) were placed in three groups of 10 mice. Groups A and B were orally administered 100 and 400 mg/kg of ethanolic extract respectively for 49 days, while group C received distilled water as the control. At expiration of the treatment, the animals were sacrificed to harvest blood and internal organs for hematologic, biochemical, antioxidant and histologic analyses. Results: Hematologic analysis revealed increased monocytes in all the groups. Biochemical and Electrolytes analytes did not show any significant difference. Antioxidant results showed a dose-dependent significant decrease in super oxide dismutase (SOD). Glutathione (GSH) revealed a significant increase in 400mg/kg. No pathology was observed in the heart, but mild toxicity in the kidneys, loss of alveoli architecture in the lungs, inflammation, and congestion of portal triads and central vein in the liver were observed. Increased monocytes observed may depict antibody and anti-infection potentials of the extract. Decreased value of RBC, Hb at higher doses, and mean corpuscular hemoglobin (MCH) at lower doses suggest hemolytic anaemia. Insignificant difference in liver enzymes may suggest extract is not hepatotoxic. Insignificant increased creatinine and urea implies that the extract is not nephrotoxic. Conclusion: The extract displayed a great potential to inhibit free radicals. It increased the antioxidant defense system and GSH at 400mg/kg. Increased SOD and catalase suggest the upregulation of protein which culminates in the clean-up of free radicals and decreasing oxidative stress, thus protecting tissues from extremely reactive radicals. https://hmj.lums.ac.ir/article_221253_911032c9b59b391fb1a97c265bd13fff.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21445420200501Physicochemical Properties of Bromelain Adsorption on Magnetic Carbon Nanoparticles and in Vitro Cytotoxicity on Breast Cancer Cells15316222125410.22087/hmj.v5i4.839ENKarimianRad FDepartment of Biology, Faculty of Science, Central Tehran Branch, Islamic Azad University, Tehran, IranRamezaniMDepartment of Biology, Faculty of Science, Central Tehran Branch, Islamic Azad University, Tehran, IranMohammadgholiADepartment of Biology, Faculty of Science, Central Tehran Branch, Islamic Azad University, Tehran, IranJournal Article20250520Background and Aim: As a proteolytic enzyme extracted from the pineapple stalk, Bromelain (Br) is known as an anticancer agent. In the first stage of this research, we studied the physicochemical factors which influence the maximum adsorption of Br on magnetic carbone nanoparticles (MCNPs) and then the anticarcinogenic activity of Br enzyme alone. Moreover, they were evaluated in combination with these particles on MCF-7 breast cancer cells. Materials and Methods: The operational determinants influencing Br adsorption such as pH, contact time (30, 60, 90, 120 and 180 min), adsorbent dosage (1 gr/L, 5 gr/L), initial Br concentration (50, 150 and 300 mg/L) and temperature (35 and 50°C) were studied in detail. Then cancer cells were exposed to various Br concentrations (0.1 μg/mL, 1 μg/mL, 10 μg/mL, and 100 μg/mL) and the cell viability was determined by methylthiazol tetrazolium (MTT) assay after 24, 48 and 72 h. Results: The highest adsorption of Br on MCNPs was 44 mg/g and was achieved at pH 5, 35°C and 120 min with 50 mg/L initial Br concentration and 1g/L MCNPs. The adsorption used the Freundlich and pseudo first-order kinetic models. The results indicated that MCNPs could be a potential effective adsorbent for the removal of Br. MTT assay indicated that a 100 μg/mL concentration of Br alone (after 24 h) and in combination with MCNPs (after 72 h) could efficiently inhibit the MCF-7 breast cancer cells. Conclusion: Although the dose of Bromelain on synthesized MCNPS is about 440 times less than Bromelain alone, it possesses a significant cytotoxicity (P<0.001). Moreover, synthesized MCNPS had a considerable advantage of slow delivery which is favorable for the treatment of cancer. https://hmj.lums.ac.ir/article_221254_1a0f36f6e92560bcae205b6c32cc033f.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21445420200501Effects of Various Drying Methods on the Quantity and Quality of Peppermint (Mentha piperita L.) Essential Oil22125610.22087/hmj.v5i4.899ENJournal Article20250520Erratum for Ghorbaniet al. Effects of Various Drying Methods on the Quantity and Quality of Peppermint (Mentha piperita L.) Essential Oil. Herb. Med. J. 2019;4(4):157–62. The institutional affiliations of the authorAzizollah Kheiryisincorrect. The correct affiliations isas follow: Department of Horticultural Sciences, Faculty of Agriculture, Universityof Zanjan, Zanjan, Iran https://hmj.lums.ac.ir/article_221256_5c9249bf2b0d32c75c3e83c4a33a8c29.pdf