Lorestan University of Medical SciencesHerbal Medicines Journal2538-21444120190501Phenolic Composition, in Vitro Antioxidant and Anticancer Activities of Hypericum Japonicum Thunb and Scoparia Dulcis L11022133110.22087/herb%20med%20j.v1i1.745ENQuang-UngLeDepartment of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Pingtung
91201, Taiwan.Horng-LiangLayDepartment of Plant Industry, National Pingtung University of Science and Technology, Pingtung 91201, TaiwanMing- ChangWuDepartment of Food Science, National Pingtung University of Science and Technology, Pingtung 91201, TaiwanJournal Article20250520Background and Aim: Hypericum Japonicum Thunb (HT) and Scoparia Dulcis L (SL) have received considerable attentions as natural products for their applications with health benefits. This study aimed at investigating the in vitro antioxidant and HepG2 (human liver cancer cell line) and A549 (adenocarcinomic human alveolar basal epithelial cell line) growth inhibitory effect of the ethanol extracts from the HT and SL.Materials and Methods: Total phenolic and flavonoid contents were calculated. Antioxidant effect was determined by measuring hydroxyl radical scavenging activity, 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS+) and 2,2’-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities. Phenolic compounds were identified by high performance liquid chromatography (HPLC) method. The activity of the apoptotic proteins Bcl-2, Bad, Bax, Caspase-3, -9 were measured by Western blotting.Results: The rosmarinic acid and chlorogenic acid were isolated from the HT. The rutin and rosmarinic acid were newly identified compounds from the SL. Moreover, results revealed that the HT extract possesses the higher levels of ABTS+, DPPH and hydroxyl radical scavenging capacity with IC50 of 45.13±0.89, 89.29±0.78 and 38.32±0.17µg/mL, respectively. The HT and SL extracts induce HepG2 growth inhibition with IC50: respectively 245.755 ± 2.23b µg/mL and 553.32 ± 14.46a µg/mL and induce A549 growth inhibition with IC50 of the HT and SL: 314.15± 5.96b µg/mL and 446.35± 0.9a µg/mL at 48 hr, respectively. The HT and SL extracts activated the apoptotic proteins including bcl-2, bad, caspase-3, -9 in HepG2; p53, caspase-3, bax and bad in A549.Conclusion: The findings strongly suggest that the HT and SL extracts could be excellent sources of antioxidants as functional food ingredients in cancer prevention and treatment https://hmj.lums.ac.ir/article_221331_4093e6e3dd3fd905a0302ff0ad607fbc.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21444120190501Cadmium Concentration in Cigarette Brands, Tobacco Leaves, and Smokers' Blood Samples111722133210.22087/herb%20med%20j.v1i1.736ENSeyed Ziyae AldinSamsam ShariatDepartment of Clinical Biochemistry, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical
Sciences, Isfahan University of Medical Sciences, Isfahan, IranAliRastqarDepartment of Psychiatry and Neuroscience, Université Laval, Québec, QC, CanadaMostafaJabbariSwedish Centre for Resource Recovery, University of Borås, Borås, SwedenMarziehTaheriIsfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of medical Sciences, Isfahan, IranMahtabKeshvariIsfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of medical Sciences, Isfahan, IranJournal Article20250520Background and Aim: The present study sought to determine cadmium (Cd) concentration in three different types of tobacco leaves, cigarette smokers' blood, and certain cigarette brands that are both imported from abroad and produced in Iran.Materials and Methods: The participants consisted of male volunteers of 40-65 years old whose blood samples were collected and categorized into four distinct groups of cigarette smokers (N=40) based on the number of cigarettes every participant would smoke per day. Serum concentrations of heavy metal were determined using graphite furnace atomic absorption (GFAA). Moreover, graphite furnace atomic absorption spectroscopy technique was used to determine all of the samples examined in this research.Results: Mean concentrations of Cd in imported cigarettes brands and cigarettes produced in Iran were 1.89±o.12 µg/g (dry weight) and 1.44±0.8 µg/g (dry weight) respectively. Average levels of Cd in smokers’ blood with 10, 20, 30, and 40 cigarettes per day were 1.31±0.14, 2.42±0.17, 3.18±0.21, and 4.38±0.18 µg per liter respectively. The mean concentrations of Cd in Hakan, Kasham and Borazjan tobacco were 2.18 ± 0.12, 2.43±0.9, and 2.89±017µ/g (dry weight) respectively.Conclusion: The data presented in this study did not indicate any significant difference between the cigarette brands produced in Iran, while Rothman cigarette brand had the highest Cd concentrations among the imported cigarettes. The blood Cd concentration in smokers that depends on the number of cigarette smoked per day was about four times higher than non-smokers. https://hmj.lums.ac.ir/article_221332_1206a2f1e2c1f1ad1c90e68726d4bddb.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21444120190501A Comparison of the Anxiolytic and Hypnotic Effects of Origanum majorana Essential Oil and Alprazolam in Male Mice182622133310.22087/herb%20med%20j.v1i1.729ENNeginManouchehriFaculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, IranSaeidAbbasi-MalekiDepartment of Pharmacology and Toxicology, Urmia Branch, Islamic Azad University, Urmia, IranZahraMousaviDepartment of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic
Azad University, Tehran, IranJournal Article20250520Background and Aim: Previous studies have reported many pharmacological properties for Origanum majorana, including anticonvulsant, analgesic, sedative, and antidepressant-like effects. Given this, the aim of this study was to compare the anxiolytic and hypnotic effects of Origanum majorana essential oil (OMEO) and alprazolam in male mice.Material and Methods: In this experimental study, 60 male NMRI mice were divided into 10 groups of six each. In both of the experiments, negative control groups received vehicle (10 ml/kg) and positive control groups received alprazolam (0.5 mg/kg) and treatment groups received OMEO (10, 20 and 40 mg/kg). For assessing anxiolytic effects, the number of entries and the time spent in the open and closed arms were recorded by using elevated plus maze (EPM). For evaluating hypnotic effects, the onset and duration of the sleep were recorded by using ketamine-induced sleeping time test. One-way ANOVA followed by post hoc Tukey's test were used for statistical analysis.Results: All doses of OMEO and alprazolam, compared to control group, increased the number of entries and the time spent in the open arms and, conversely, reduced the number of entries and the time spent in the closed arms. In addition, all doses of the essential oil similar to alprazolam reduced the onset of sleeping and increased the duration of sleep.Conclusion: The results relieved that the OMEO have anxiolytic and hypnotic effects. However, further studies are needed to determine their exact mechanism of action. https://hmj.lums.ac.ir/article_221333_e13640f4976a0907fc7c1d234ff6494d.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21444120190501The Solid-Phase of the Dynamic Microextraction of Thymol and Carvacrol Using a Porous, Low-Cost and Unbreakable Disk Coated by Polythiophene/Multiwalled Carbon Nanotubes274322134310.22087/herb%20med%20j.v1i1.721ENFatemehYazdankhahDepartment of Chemistry, Lorestan University, Khorramabad, IranJournal Article20250520Background and Aim: In the present study, a stainless steel plate was penetrated and platinized, and it was used as the disk for the solid phase dynamic extraction (SPDE) method. The SPE disk was coated by polythiophene/multiwalled carbon nanotubes (PTh/MWCNTs) nanocomposite using in-situ electropolymerization method.Materials and Methods: A high-surface area, porous and unbreakable platinized SPE disk was used for the dynamic extraction and determination of thymol and carvacrol by high-performance liquid chromatography (HPLC) with UV detection. The effect of various variables, including the type of elution solvent, pH, ionic strength and extraction time was optimized by the use of the central composite design (CCD) method.Results: Under the response surface methodology, the present method has admissible calibration curves over wide linear ranges of 0.01-100 µg mL-1 with high coefficient of determination (0.9998) that firmly confirms the high pertinence of the present method to the quantification of analytes. Meanwhile, their limits of detection (LODs) were 0.007 and 0.005 µg mL-1, and the limits of quantification (LOQs) were 0.09 and 0.01 µg mL-1 for thymol and carvacrol, respectively. Moreover, the enrichment factor (EF) was 87 for thymol and 54 for carvacrol, and the preconcentration factor was 142.8 for thymol and carvacrol.Conclusion: The SPDE-PTh/MWCNTs-disk was used for the extraction of thymol and carvacrol in thyme samples, and satisfactory results were obtained https://hmj.lums.ac.ir/article_221343_a6924164599b66b4fa1293d1a99d0370.pdf