Lorestan University of Medical SciencesHerbal Medicines Journal2538-21442320170501The Antioxidant Properties and Chemical Composition of Stachys inflata Benth Leaves Grown Wild in Lorestan Province9710422154210.22087/hmj.v0i0.623ENHassanAhmadvand1 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
2 Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, IranSimaFarajollahiDepartment of Biology, Islamic Azad University, Boroujerd Branch, Boroujerd, IranHamzeAmiriDepartment of Biology, Faculty of Basic Sciences, Lorestan University, Khorramabad, IranAliAmiriDepartment of Internal Medicine, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, IranJournal Article20250524Background and Aim: In recent years there has been an increasing interest in herbal medicines and natural antioxidant products to be used as medicines and food additives. The present research identified the various antioxidative activities of ethanol and methanol extracts, and chemical composition of Stachys inflate Benth leaves essential oil.Materials and Methods: This experimental study was carried out in 2015 in Lorestan University of Medical Sciences. Ethanol and methanol extracts of Stachys inflata Benth leaves were primarily prepared. The total antioxidant capacity of samples was assessed by phosphomolybdate method. Total phenol content and total flavonoid content were determined by Folin-Ciocalteu and Zhishen methods. The components of Stachys inflate Benth leaves essential oil were analyzed with gas chromatography/mass spectrometry (GC/MS).Results: It was demonstrated that the total antioxidant capacity of ethanol and methanol extracts of Stachys inflata Benth leaves were 4.30±0.17; 3.63±0.23 mg of ascorbic acid equivalents /g of extract. Moreover, the total phenol content ethanol and methanol extracts of Stachys inflata Benth leaves were 830.334±26.41; 384.29±13.37mg of gallic acid equivalents /g of extract, and flavonoid content ethanol and methanol extracts of Stachys inflata Benth leaves were 17.09±0.154; 31.18±1.03 mg of quercetin equivalents /g of extract. GC/MS data and retention indices of Stachys inflate Benth leaves essential oil samples were used to identify 54 constituents. These compounds make up a total of 48.87 percent essential oil. Phenol, 2-methyl-5(1-methyleth; Phenol, 5-methyl-2-(1methyleyth; 2-pentadecanone and hexadecanoic acid are the major compounds of Stachys inflate Benth leaves essential oil. This study indicated that Stachys inflate Benth leaves essential oil has remarkable antioxidant properties. Furthermore, Stachys inflate Benth leaves essential oil was identified as an easily accessible source of natural antioxidants such as Phenol,2-methyl-5(1-methyleth; Phenol,5-methyl-2-(1methyleyth; 2-pentadecanone,6,10,14-trimet; Veridi florol; 2-cycohexen-1-on,2-methyl-5- and 1H-cycloprop[e]azulen-7-ol,dec. It may also be suitable to be used in food and pharmaceutical applications.Conclusion: This study demonstrated that Stachys inflata Benth leaves are easily accessible sources of natural antioxidants. Therefore, they may be suitable to be used in food and pharmaceutical applications.https://hmj.lums.ac.ir/article_221542_9d147fc9049026a91e133498db2877d8.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21442320170501The Effects of Olive Leaf Extract Administration on the Cerebral Hypoperfusion-Induced Electrophysiological Alterations in Rat Heart105113221544ENMehrnoushMoghaddasi1 Department of Physiology, Lorestan University of Medical Sciences, Khorramabad, Iran
2 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranMajidTaatiDepartment of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, IranHasanNoroozi1 Department of Physiology, Lorestan University of Medical Sciences, Khorramabad, Iran
2 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranSamBamani LirgeshasiStudent Research Committee, Lorestan University of Medical Sciences, Khorramabad, IranSoheilaPourkhodadadRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranBahramDelfanRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranAfshinNazari1 Department of Physiology, Lorestan University of Medical Sciences, Khorramabad, Iran
2 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IraJournal Article20250524Background and Aim: In the present study, the role of ethanolic extract of olive (Olea europaea) leaf administration against the cerebral hypoperfusion-induced electrophysiological alterations and left ventricular thickness in rat heart was studied.Materials and Methods: The right carotid artery was occluded with a 5-0 silk suture and the left carotid artery was occluded after a week. The olive (Olea europaea) leaf extract was administered orally (by gavage) to three experimental groups of rats in 100, 200 and 300 mg/kg/day for two weeks after the left carotid artery occlusion and processed in 25 days. The sham and hypoperfusion groups were fed with normal saline. Each group had 6 rats. After 25 days, electrophysiological heart parameters, i.e. HR, QTc, QRS duration, QT interval, PR interval, RR interval, ST amplitude, T wave amplitude and also the thickness of left ventricle (TLV) were assessed.Results: The administration of olive leaf extract (100, 200 and 300 mg/kg) exhibited its cardioprotective effect by both stabilizing heart rate and preventing ECG changes (such as shortening of PR, QRS, QT and QTc intervals, prominent T waves and ST elevation). Moreover, olive leaf extract (100, 200 and 300 mg/kg) prevented cardiac hypertrophy after cerebral hypoperfusion.Conclusion: Our results indicated that post-treatment with ethanolic extract of olive leaves could prove beneficial effects in managing the cerebral hypoperfusion-induced cardiac abnormality in rats.https://hmj.lums.ac.ir/article_221544_c4071816f4128416ecad119f96fcbc35.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21442320170501The Effect of Rosmarinus officinalis L Extract on the Inhibition of High Glucose-Induced Neurotoxicity in PC12 Cells: an In Vitro Model of Diabetic Neuropathy11412122154710.22087/hmj.v0i0.636ENMarziehRashidipourRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranZahraHajializadehEndocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical
Sciences, Kerman, IranSaeedEsmaeili-MahaniDepartment of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, IranAyatKaeidi4 Physiology-pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
5 Department of Physiology and Pharmacology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, IranJournal Article20250524Background and Aim: Diabetes mellitus and continued hyperglycemic condition prompt serious diabetic complications such as diabetic neuropathy. Numerous studies have shown the involvement of oxidative stress and high glucose-induced cell death in the progress of diabetic neuropathy. Rosmarinus officinalis L has been suggested in the literature as an anti-diabetic herbal medicine that contains potent antioxidant components. Therefore, the neuroprotective effect of Rosmarinus officinalis L (RE) extract was investigated in glucose-induced neurotoxicity via pheochromocytoma )PC12( cells as an appropriate in vitro model of diabetic neuropathy.Materials and Methods: Cell viability was determined using MTT assay. Cleave caspase-3 and Bax: Bcl2 ratio, as biochemical parameters of cellular apoptosis, were measured by western blotting analysis.Results: Our data showed that a 4-fold elevation in the medium glucose significantly reduced cell viability (P < 0.01) and increased caspase-3 activation (P < 0.01) as well as Bax: Bcl2 ratio (P < 0.05) in PC12 cells after 24 h. Incubation of high glucose medium cells with 60μg/ml RE extract decreased high glucose-induced cell toxicity and prevented caspase-3 activation and Bax: Bcl2 ratio.Conclusion: It could be concluded that RE extract is effective in the protection against hyperglycemia-induced cellular toxicity. This could be relevant to the prevention of apoptosis. Moreover, the results suggest that RE has the therapeutic potential to attenuate diabetes complications such as that neuropathy.https://hmj.lums.ac.ir/article_221547_244ecf7a2b387a51ea4f0532d9464677.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21442320170501Antileishmanial Activity of Myrtle Methanolic Extract against Leishmaniamajor: an In Vitro Study12212522154910.22087/hmj.v0i0.637ENHosseinMahmoudvandRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranHamedSalehi LalehmarziBogomolets National Medical University, Kyiv, UkraineShirzadFallahiRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranMaryamMoslehi Baharanchi3 Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, IranPardisBayat3 Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, IranSarehJahanbakhshBam University of Medical Sciences, Bam, IranJournal Article20250524Background and Aim: In this study we assessed the in vitro antileishmanial activity of myrtle (Myrtus communis L.) methanolic extract against Leishmania major.Materials and Methods: The in vitro antileishmanial effects of myrtle methanolic extract against L. major promastigote and amastigotes were determined by colorimetric cell viability (MTT) assay and macrophage model, respectively. The IC50 values were also calculated by probit test in SPSS software.Results:The obtained results showed that myrtle extract was significantly inhibited promastigote growth of L. major based on a dose and time dependent manner. The measured IC50 values for myrtle methanolic extract and MA as control drug against promastigote forms of L. major were 23.6 µg/mL and 88.3 µg/mL, respectively. The obtained IC50 values were 13.8https://hmj.lums.ac.ir/article_221549_8c1327efea64c02e376c026a8a696b38.pdf