Lorestan University of Medical SciencesHerbal Medicines Journal2538-21442120170501Effects of Caffeic Acid on Serum Lipid Profile and Atherogenic Index in Alloxan-Induced Diabetic Rats3822156310.22087/HMJ.V1I2.591ENHassanAhmadvand1 Razi Herbal Medicines Researches Center, Lorestan University of Medical Sciences, Khorramabad, Iran
2 Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, IranTayebehDavoodiDepartment of Biochemistry, Payame Noor University, Tehran, IranEsmaeelBabaeenezhadStudent Research Commititee, Lorestan University of Medical Sciences, Khorramabad, IranJournal Article20250524Background and Aim: Diabetes is a major public health problem worldwide. Oxidative stress is a risk factor in diabetes. The aim of this study is to evaluate the possible beneficial effect of caffeic acid (CA) on serum lipid parameters and atherogenic index in alloxan-induced male diabetic rats.Materials and Methods: 40 male Wistar rats were randomly divided into 4 groups: group I as the healthy control group, group II as the healthy group treated with CA (50 mg/kg i.p. daily), group III as the diabetic control group and group IV as the diabetic group treated with CA (50 mg/kg i.p.daily). Diabetes was induced in the 3rd and 4th groups by the injection of alloxan monohydrate (100 mg/kg s.c). After seven weeks, animals were anaesthetized and blood samples were collected. Then, the serum level of fasting blood glucose (FBS), lipid parameters and the atherogenic index were measured.Results: The serum level of FBS, cholesterol, low density lipoprotein (LDL-C) and atherogenic index significantly decreased in the diabetic group treated with CA compared with the untreated diabetic group. The serum level of high density lipoprotein (HDL-C) significantly increased in the treated diabetic group compared with the untreated diabetic group.Conclusion: The results of this study indicated that CA has beneficial effects on serum blood glucose, lipid profile and atherogenic index in type 1 diabetic rats.https://hmj.lums.ac.ir/article_221563_daa66fb1243b7c6b4a12693be728bf8a.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21442120170501Betaine improves gastroprotective effects of ranitidine and omeprazole against Indomethacin-induced gastric ulcer in rats91722156410.22087/HMJ.V1I2.592ENMasoudAlirezaeiDivision of Biochemistry, School of Veterinary Medicine, Lorestan University, Khorramabad, IranVahidJaldani2. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
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Islamic Azad university, Sanandaj Branch, Sanandaj, IranOmidDezfoulian4 Department of Pathobiology, School of Veterinary Medicine, Lorestan University, Khorramabad, IranGholamrezaShahsavariRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranJournal Article20250524Background and Aim: Antioxidant capacity of betaine has been indicated in our recent studies. Thus, we examined oral betaine as an antioxidant agent in combination with antisecretory drugs to prevent indomethacin-induced gastric damages in rats.Materials and Methods: Fifty-six adult male Sprague–Dawley rats were divided into two controls (negative and normal) and five experimental groups as follows: betaine-indomethacin (Bet.-Ind.), ascorbic acid-indomethacin (Asc.-Ind.), omeprazole-indomethacin (Ome.-Ind.), betaine-omeprazole plus indomethacin (Bet.-Ome.-Ind.) and betaine-ranitidine plus indomethacin (Bet.-Ran.-Ind.).Results: The betaine pretreated groups received betaine at a dosage of 1.5% (w/w) in their diet, whereas 50 mg/kg of ascorbic acid was administered orally to the Asc.-Ind., group for 15 consecutive days. After a 24 hour fast, all the groups received 48 mg/kg of indomethacin once except for normal control group. The omeprazole and ranitidine groups also received one dose of omeprazole (10 mg/kg) and ranitidine (50 mg/kg), 120 minutes before receiving indomethacin. Histopathological findings indicated the gastroprotective effects of betaine and ranitidine in pretreated rats. Pretreatment by betaine and ranitidine increased significantly the ulcer index inhibition (%), in comparison with ascorbic acid and omeprazole (alone) treatment. Glutathione peroxidase (GPx) activity was significantly higher in the Bet.-Ran.-Ind., group as compared to the Asc.-Ind., and Ome.-Ind., treated rats. GPx activity also increased significantly in Bet.-Ind., treated rats as compared to the Asc.-Ind. group. Catalase (CAT) activity was remarkably higher in the Bet.-Ran.-Ind., treated rats than the Asc.-Ind., and Ome.-Ind., groups. TBARS concentration as a lipid peroxidation marker increased significantly in Ome.-Ind., group as compared to the Bet.-Ind., and Bet.-Ran.-Ind., treated rats.Conclusion: Thus, it seems that betaine as an antioxidant agent, is able to improve the effects of ranitidine and omeprazole against indomethacin-mediated gastric damages in rats. It may also be promising in the prevention of NSAIDs side effects.https://hmj.lums.ac.ir/article_221564_7c42478604edec00caad68e6003bc0db.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21442120170501Effects of 3, 4-Dihydroxyflavone on Cryopreserved Testicular Tissue of Neonatal Mouse182522156510.22087/HMJ.V1I2.593ENAfshinPirnia1 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranAbolfazlZendedel1 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranAbolfazlAbbaszadeh1 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranKhaterehAnbariSocial Determinant of Health Research Center, Lorestan University of Medical Sciences, Khorramabad, IranArmanMousaviRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranMohammadrezaGholamiDepartment of Anatomical Sciences, Lorestan University of Medical Sciences, Khorramabad, IranJournal Article20250524Background and Aim: Infertility is a side effect of cancer treatment because of chemotherapy or radiotherapy. Cryopreservation of testicular tissue or spermatogonial stem cells before cancer treatment and their transplantation may preserve the natural fertility. Cryopreservation is a damaging process due to free radicals and toxic effect of frozen solution. The purpose of this paper is to study the antioxidant and antiapoptotic effects of 3',4'-dihydroxyflavone on the structure of frozen-thawed testicular tissue of neonatal mouse.Materials and Methods: Testes of 6-day-old NMRI mice (N = 20) were isolated. Testicles were randomly divided into four groups: two groups as control groups for quick and slow freezing-melting process, and two others as treatment groups that underwent quick and slow freezing-melting with addition of 3',4'-dihydroxyflavone (10µM) in frozen solution. For the assessment of structural changes and apoptosis in the frozen-thawed testes, the hematoxylin-eosin staining and tunnel pod kits were used respectively. In order to analyze the data, we used Kruskal-Wallis Test and Mann-Whitney Test. The statistical analysis was entirely done by SPSS software.Results: Histopathological changes and apoptosis rate were significantly decreased after thawing in both treatment groups in comparison with control groups.Conclusion: Adding 3',4'-dihydroxyflavone (10µM) to freezing-melting environment and the use of quick freezing-melting method can reduce the histopathological and apoptotic changes.https://hmj.lums.ac.ir/article_221565_49ddb530d11a9e7d13217e3ed243e2a7.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21442120170501Bioassay Screening of the Essential Oil and Various Extracts of Nigella sativa L. Seeds Using Brine Shrimp Toxicity Assay263122156610.22087/HMJ.V1I2.578ENFaribaSharififarDepartment of Pharmacognosy, School of Pharmacy, Kerman University of Medical Sciences, Kerman, IranAliAssadipourDepartment of Pharmacognosy, School of Pharmacy, Kerman University of Medical Sciences, Kerman, IranMohammad HassanMoshafiDepartment of Pharmaceutical Science, School of Pharmacy, Kerman University of Medical Sciences, Kerman, IranFahimehAlishahiDepartment of Pharmacognosy, School of Pharmacy, Kerman University of Medical Sciences, Kerman, IranHosseinMahmoudvandRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranJournal Article20250524Background and Aim: Since cytotoxicity screening is the first step necessary for any new drug development, this study was designed to find out and compare the cytotoxicity effects of the essential oil and various extracts of Nigella sativa L. seeds using Brine Shrimp Lethality (BSL) assay.Materials and Methods: Essential oils and various extracts of N. sativa were assessed by two methods of disk and solution of BSL. Data analysis was carried out using SPSS statistical package version 17.0 (SPSS Inc., Chicago, IL, 250 USA). Data were processed in probit-analysis program to estimate LC50 values.Results: All of the tested fractions demonstrated more cytotoxicity in the solution method. Petroleum ether and chloroform extract of N. sativa showed the most cytotoxicity with LC50 values 7 and 21 μg/ml respectively; while aqueous and ethanolic had no significant cytotoxicity. Moreover, the GC/MS analysis of the essential oil of N. sativa showed the p-cymene (48.1%), α-thujone (14.38%) and dihydro carveol (9.11%) as the main compounds.Conclusion: These results suggest some limitation for using this spice in diet. Furthermore, this plant could be considered as a source of cytotoxic compounds which should be studied in details.https://hmj.lums.ac.ir/article_221566_4abe72e122b24d6565b4c69eb232df23.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21442120170501The Extraction and Determination of Ellagic Acid Content in the Peels of Six Iranian Pomegranates Cultivars Using a New Miniaturized Matrix Solid-Phase Dispersion Method323822156710.22087/HMJ.V1I2.575ENFaribaNazari SerenjehDepartment of Chemistry, Faculty of Science, Lorestan University, Khorramabad, IranPayman HashemHashemDepartment of Chemistry, Faculty of Science, Lorestan University, Khorramabad, IranJournal Article20250524Background and Aim:The peels of six Iranian pomegranates (Punica granatum L.) cultivars, as a traditional medicine, were treated with a new miniaturized matrix solid-phase dispersion (MSPD) method for the HPLC determination of ellagic acid (EA).Materials and Methods:In the proposed method, only 10mg of the sample powder was ground and blended with an equal amount of C18 sorbent in an agate mortar. The use of the agate mortar with smooth surface facilitated the sample transfer into a cartridge and reduced the required amount of sample and sorbent. Micro volumes of dichloromethane, n-hexane and methanol were used as modifier, washing and elution solvents, respectively. The eluate was injected into an HPLC-UV system for the analysis.Results:Several factors such as the type and amount of dispersing sorbent, modifier, washing solvent and eluent were carefully studied and optimized. Six replicated analyses at the optimized conditions resulted in a recovery of 96.7% and a relative standard deviation of 5.87%. The proposed method was successfully applied to the extraction and determination of EA in the peels samples.Conclusion:According to the ultimate results, the MSPD method is an efficient technique for the quantitative extraction of EA from the peels of pomegranate. Malas cultivar has the highest amount (18.1 g kg-1) of ellagic acid content compared to the other studied pomegranate cultivars.https://hmj.lums.ac.ir/article_221567_bb4c59b204f73884d4df6d00d70642e5.pdf