Lorestan University of Medical SciencesHerbal Medicines Journal2538-21441120160501The Antioxidant and Antiangiogenic Effects of Dietary supplement of Nigella sativa Crude Oil on Breast Tumor in BALB/c Mice110221570ENSimaBahramianDepartment of Physiology, Faculty of Biological Sciences and Technologies, Shahid Beheshti University, G.C., Tehran, IranMohammad RezaBigdeliDepartment of Physiology, Faculty of Biological Sciences and Technologies, Shahid Beheshti University, G.C., Tehran, IranBahramRasoulianRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranJournal Article20250524Background and Aim: Cancer embraces a vast range of diseases including that of the breast and is triggered by various factors such as reactive species of oxygen. Angiogenesis, then, aids the cancerous mass to meet its needs and develop further. Nigella sativa L. (NS), traditionally cultivated and consumed in the Middle East, has long been appreciated for its medicinal benefits. In this study, we aimed to look into its antioxidant and anti-angiogenic effects on breast tumor in BALB/c mice.Materials and Methods: 3 groups of BALB/c mice, respectively received 1, 2 and 4 ml/kg/day of NS crude oil via gavaging for 4 months before breast tumor transplantation, while the control mice were gavaged with distilled water. Then tumor volume, the activity of antioxidant enzymes, the amounts of VEGF and endostatin were studied to examine NS crude oil’s impact against breast tumor.Results and Conclusions: Our findings revealed that the mice pretreated with 4 ml/kg/day of NS oil had significantly smaller tumor volumes, higher SOD and CAT activity, reduced VEGF and increased endostatin amounts. So, we came to this that Nigella sativa crude oil seems to inhibit breast tumor growth in part by improving the activity of antioxidant enzymes and in part by interfering with angiogenesis. More studies are, yet, required to better illuminate its other mechanisms of involvement.https://hmj.lums.ac.ir/article_221570_588e6ad8f24746d5d8a2fe43b7d9d25e.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21441120160501The Cytotoxic and Antileishmanial Effects of Satureja khuzestanica Essential Oil1117221573ENFarnazKheirandishRazi Herbal Medicines Research Center, Department of Parasitology and Mycology, School of Medicine, Lorestan University of
Medical Sciences, Khorramabad, IranRouzbehChegeniMichener Institute of Education at University Health Network, Toronto, Ontario, CanadaBahramDelfanDepartment of Parasitology and Mycology, Faculty of Medicine, Razi Herbal Medicines Research Center, Lorestan University of
Medical Sciences, Khorramabad, IranMahboubehJabariRazi Herbal Medicines Research Center, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, IranFarzadEbrahimzadehDepartment of Biostatistics, Faculty of Health and Nutrition, Lorestan University of Medical Sciences, Khorramabad, IranMarziehRashidipourEnvironmental Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, IranJournal Article20250524Background and Aim: Leishmaniasis is endemic in 98 countries including Iran. Pentavalent antimony compounds resistance as first-line therapy is increasing in some local areas. Also side effects of these drugs are limited at the beginning of treatment, but the toxicity increases with time. The aim of this study was to assess the effect of Satureja khuzestanica essential oil (SKEO) on promastigote and amastigote Leishmania major forms.Materials and Methods: The components of S. khuzestanica oil were identified by gas chromatography/mass spectroscopy (GC/MS) analysis. To evaluate antipromastigote activity the different concentrations of extract and glucantime were added to the wells that contained L. major. The plates were incubated at 26±1°C for a week. On days 1, 3 and 5, the number of live promastigotes in each well was counted. For assessment of SKEO effect on intracellular amastigotes, mouse peritoneal macrophages were isolated and infected with promastigotes. Different concentrations of the extract and glucantime were added to the cultures. The cultures were incubated at 37°C and CO2 5%. The number of infected macrophages and amastigotes within each macrophage were counted. Toxicity assessment of SKEO on macrophages was done by MTT method.Results and Conclusions: The mean number of promastigotes, infected macrophages and amastigotes in a macrophage in the control and treated groups had significantly difference. So that their number in the treated groups was less as a dose-dependent response. The present research showed potent antileishmanial activity of, SKEO; additionally this plant had no toxic effect on mammalian cells.https://hmj.lums.ac.ir/article_221573_d7a3d2fcb9b703e728cceaadc7d20128.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21441120160501Oleuropein Reduces Cisplatin-Induced Nephrotoxicity in Human Embryonic Renal Epithelial “GP-293” Cells1823221574ENAyatKaeidiDepartment of Physiology and Pharmacology, Rafsanjan University of Medical Sciences, Rafsanjan, IranMaryamRezaeiRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranMarziehRashidipourRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranJournal Article20250524Background and Aim:The protective effect of oleuropein,a chief polyphenol compound in olive tree, on cisplatin-induced nephrotoxicity in human cultured renal tubular cells, GP-293, was investigated.Materials and Methods:Cell viability was determined by the MTT assay. Cleaved caspase-3 and Bax: Bcl2 ratioas biochemical parameters of cellular apoptosis, was assessed using western blot analysis.Results:Our data showed that cisplatin in the dose of 55µg/ml significantly can reduced cell viability, increased caspase-3 activation and Bax: Bcl2 ratio in GP-293 cells after 24hr. Incubation of the cisplatin treated cells, with 20μg/ml oleuropein decreased the cisplatin-induced cell toxicity, as well as prevented caspase-3 activation and reduced Bax: Bcl2 elevation ratio.Conclusions: Oleuropein protects against cisplatin-induced cellular toxicity. This could be associated with the prevention of cellular apoptosishttps://hmj.lums.ac.ir/article_221574_c68cce0f709db5a976ee5324bbdaec98.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21441120160501The Antileishmanial Activity of Essential Oils from Some Traditionally Used Medicinal Plants in Iran2428221575ENHosseinMahmoudvand1Department of Parasitology and Mycology, School of Medicine, Razi Herbal Medicines Research Center, Lorestan University of
Medical Sciences, Khorramabad, IranBehrouzEzatpour1Department of Parasitology and Mycology, School of Medicine, Razi Herbal Medicines Research Center, Lorestan University of
Medical Sciences, Khorramabad, IranSarehJahanbakhsh2Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, IranJournal Article20250524Background and Aim: Cutaneous leishmaniasis (CL) is the most common type of leishmaniasis affecting 1.5 million people through the world annually. Treatment of CL by pentavalent antimony compounds remains a challenge because of adverse side effects. The present study was designed to evaluate the in vitro antileishmanial properties of six essential oils from selected spices and herbs ethno-medicinally used in Iran against Leishmania tropica and L. major promastigotes.Materials and Methods: The in vitro antileishmanial effects of selected medicinal plants against L. tropica and L. major promastigotes were evaluated by colorimetric cell viability (MTT) assay. The IC50 values were also calculated by probit test using SPSS software.Results: The findings demonstrated that all the tested essential oils had inhibitory effects on promastigote growth of L. tropica with IC50 values ranging from 3.2µg/mL to 19.3µg/mL and 2.7µg/mL to 18.8µg/mL for L. tropica and L. major, respectively. Zataria multiflora Boiss essential oil significantly (P<0.05) was much more effective than essential oils of the other tested plants and control drugs once they demonstrated lower IC50 values promastigote form.Conclusions: The findings of present study indicated antileishmanial effects of some Iranian medicinal plants particularly Z. multiflora Boiss. However, further studies, on the animal models as well as volunteer human, are needed to confirm these results.https://hmj.lums.ac.ir/article_221575_83af19d599eaf668464305e40584ee87.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21441120160501Chemical Composition, in Vitro Antibacterial and Cytotoxicity Effect of Nectaroscordum tripedale Extract2936221576ENBehrouzEzatpourRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranMehdiAzamiSkin Disease and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, IranMasoumehMotamediRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranMarziehRashidipourYoung Researchers and Elite Club, Islamic Azad University, Khorramabad Branch, Khorramabad, IranHosseinMahmoudvandDepartment of Medical Parasitology and Mycology, Lorestan University of Medical Sciences, Khorramabad, IranMasoudAlirezaeiDivision of Biochemistry, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, IranMojganAzadpourRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranJournal Article20250524Background and Aim: The present study investigated the antimicrobial, and cytotoxic activities of the methanol extract of Nectaroscordum tripedale leaves.Materials and Methods: Methanolic extract of Nectaroscordum tripedale was investigated for its phytochemical components, antimicrobial activity and cytotoxicity. The antibacterial potentialities of methanol extract of Nectaroscordum tripedale leaves were investigated by the disc diffusion and broth dilution method against five bacterial isolates including three food-borne pathogens (Staphylococcus aureus, Listeria monocytogenes and Escherichia coli) and two healthcare-associated pathogens (Methicillin resistance Staphylococcus and Pseudomonas aeruginosa) and cytotoxicity activity were evaluated on acute myeloid leukemia cell line (KG-1a) and normal lymphocyte cells and the effect of Nectaroscordum tripedale extract and methotrexate in 0.01, 0.1, 1, and 2 mg/ml concentrations on these cells were compared.Results: The extract was found to contain 27 chemical compounds. The maximum zone of inhibition was observed in Methicillin Resistant Staphylococcus aureus (MRSA). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Nectaroscordum tripedale for E. coli and MSRA were similar (166 and 322 µg/ml, respectively). While the high level of MIC and MBC is related to Pseudomonas aeruginosa. The percentage of lysis for extract and methotrexate on the KG-1a was seen after 24 hours.Conclusions: In comparison with methotrexate, Nectaroscordum tripedale had minimal effect on normal cells. The present study revealed that the methanol extract of Nectaroscordum tripedale leaves has significant antibacterial activities along with moderate cytotoxicity's on the cancer cells that may lead to new drug development.https://hmj.lums.ac.ir/article_221576_3f756d0376fdf2915c78e73dc5f63794.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21441120160501Pretreatment with Olive Leaf Extract Improves Renal and Liver Antioxidant Systems Following Renal Ischemia-Reperfusion Injury in Rats374622157710.22087/hmj.v1i1.563ENLeilaJafaripourDepartment of Anatomy, Faculty of Medicine, Dezful University of Medical Sciences, Dezful, Iran
2 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IraBahramRasoulianRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranMajidTavafiDepartment of Anatomy, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, IranHoushangRafighdoostDepartment of Anatomy, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, IranMonaMahmodi5 Falavarjan branch, Islamic Azad University, Isfahan, IranMarzyehRashidipourKhorram Abad Branch, Young Researchers Club, Islamic Azad University, Lorestan, IranHassanAhmadvand7 Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khoramabad, Iran.Journal Article20250524Background and Aim: The effect of Olive leaf extract (OLE) as a natural antioxidant was investigated on renal and liver antioxidant enzyme activities, after renal ischemia-reperfusion (IR) in a rat model.Materials and Methods: Forty male rats divided into 5 groups: Sham (no IR), 3 "OLE+IR" groups (25mg/kg, 50mg/kg and 100mg/kg oral OLE once daily for 2 weeks, prior to renal IR) and "water+IR" group (oral gavage of water prior to IR). In the last 4 groups, both renal pedicles clamped for 45 minutes followed by 24 hours of reperfusion. Clearance of creatinine was measured. Renal and liver catalase and glutathione peroxidase activities and glutathione level were assessed as well. Volume densities of the renal proximal convoluted tubules were measured stereologicaly.Results: Glutathione level, catalase and glutathione peroxidase activities, clearance of creatinine and volume density of the proximal convoluted tubules decreased in "water+IR" compared to the sham group. Pretreatment with OLE in 3 different doses significantly increased renal antioxidant enzymes, creatinine clearance and volume density of the proximal convoluted tubules in comparison with "water+IR" group. Pretreatment with only one dose (25mg/kg) of OLE significantly increased liver glutathione and GPx compared to the "water+IR" group. Pretreatment with 2 doses (25mg/kg, 50mg/kg) increase liver catalase compared to the water+IR group.Conclusions: Two weeks of oral pretreatment with different doses of OLE decreased renal injury couesd by renal IR. This was associated with increased renal catalase and glutathione peroxidase activities. Some doses of OLE were also associated with increased liver antioxidant system parametes.https://hmj.lums.ac.ir/article_221577_f07e911d46314ccb8f06a7ac863159df.pdfLorestan University of Medical SciencesHerbal Medicines Journal2538-21441120160501Chemical Composition, Antioxidant and Antimicrobial Activities of Essential Oil from Leutea kurdistanica mozaff475222157810.22087/hmj.v1i1.564ENNaserKarimiLaboratory of Plant Physiology, Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran.IrajSalimikia2Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
3Department of Pharmacognosy, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran.ParvinRamakResearch Division of Natural Resources Research and Education Center, AREEO, Khorramabad, IranZhalehSoheilikhahLaboratory of Plant Physiology, Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran.MohammadShamizadehDepartment of Analytical Chemistry, Razi University, Kermanshah, Iran.Mohammad BagherGholivandDepartment of Analytical Chemistry, Razi University, Kermanshah, Iran.Journal Article20250524Background and Aim: Leutea kurdistanica mozaff. (Apiaceae) is a perennial herb endemic to Kurdistan province of Iran. The plants of this family are known for diversity in essential oils and economic importance. The goals of the present study were to evaluate chemical composition, antioxidant and antimicrobial activities of essential oils of L. kurdistanica.Materials and Methods: GC-FID and GC-MS were used for compositional analysis of the essential oils.Antioxidant activities of the oil were assessed using DPPH free radical test. Antibacterial and antifungal activities of the oils were assessed by disc diffusion and agar dilution methods.Results: A total of 33 compounds representing 97.3-94.2% of the essential oils were identified. Limonene (25.3%) was the main compound of the oil followed by γ–terpinene (18.1%), elemicin (15.4%) and Δ-3-carene (8.2%). In DPPH assay, the oil has radical scavenging activity with IC50 value of 51.4±2.3. The results of antimicrobial tests showed that the oil had remarkable inhibitory effects on the growth of Gram-positive bacteria and fungal strains.Conclusions: Regarding to significant antioxidants and antimicrobial activities of the oil of L. kurdistanica, these oils could be consider as a natural preservative in food and other industries. Although more investigation is needed to clarify the exact compounds responsible for observed biological activities.https://hmj.lums.ac.ir/article_221578_4c8308d42759d707b4bcd8f0f89cf595.pdf