Anti-inflammatory properties of chitosan hydrogel containing Satureja khuzestanica jamzad essential oil on the animal model

Marzieh Rashidi Pour; Esmaeel  Babaeenezhad; Samaneh  Hadavand; Marziesana  Elahian; Asma  Alekasir; Mojtaba  Khaksarian*

doi:10.22087/hmj.v8i3.1017

Marzieh Rashidi Pour Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Esmaeel Babaeenezhad Nutritional Health Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Samaneh Hadavand
Marziesana Elahian
Asma Alekasir
Mojtaba Khaksarian*

DOI: https://doi.org/10.22087/hmj.v8i3.1017

Keywords: Hydrogel, Satureja Khuzestanica, Carvacrol, Chitosan, Formalin test

Abstract

Introduction: Nanoparticles as a shell protect bioactive compounds against the environmental conditions of light, heat and oxygen and are also used as a carrier for their targeted delivery. In this study, the anti-inflammatory effects of chitosan-based nanogel containing Satureja Khuzestanica essential oil (SKE:NP) were studied in comparison with Satureja Khuzestanica essential oil (SKE). Materials and methods: nanoparticles were prepared by ionic gelation method. Anti-inflammatory effects of chitosan-based hydrogel containing Satureja Khuzestanica essential oil, using the paw inflammatory model induced with 2.5% formalin at different times (0, 1, 2, 3, 4 and 24 hours after subcutaneous injection) was evaluated. Findings: Nanoparticles with a particle size of 552.8 nm, surface charge of 24.3 and encapsulation efficiency of 30.74% were made. The results of the study showed that SKE:NP (50 mg/kg) reduces inflammation at all times (0, 1, 2, 3, 4 and 24 hours after subcutaneous injection). Hind paw was compared diclofenac sodium at times (0, 1, 2, 3, 4 hours after subcutaneous injection) caused a significant decrease the mice had paw inflammation, so that the lowest amount belonged to the group receiving nanogel 50 mg (0.208 g/cm3). On the other hand, the amount of paw swelling in the group receiving SKE with a dose of 100 mg/kg two hours after the applied treatment was 0.441 g/cm3 (P<0.0001). Conclusion: Comparison of the results showed that SKE:NP had more anti-inflammatory effects than SKE, which needs more investigation to explain the exact mechanism of this anti-inflammatory effect. Keywords: Hydrogel, Satureja Khuzestanica, Carvacrol, Chitosan, Formalin test

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