Anti-inflammatory properties of chitosan hydrogel containing Satureja khuzestanica jamzad essential oil on the animal model
Abstract
Introduction: Nanoparticles as a shell protect bioactive compounds against the environmental conditions of light, heat and oxygen and are also used as a carrier for their targeted delivery. In this study, the anti-inflammatory effects of chitosan-based nanogel containing Satureja Khuzestanica essential oil (SKE:NP) were studied in comparison with Satureja Khuzestanica essential oil (SKE). Materials and methods: nanoparticles were prepared by ionic gelation method. Anti-inflammatory effects of chitosan-based hydrogel containing Satureja Khuzestanica essential oil, using the paw inflammatory model induced with 2.5% formalin at different times (0, 1, 2, 3, 4 and 24 hours after subcutaneous injection) was evaluated. Findings: Nanoparticles with a particle size of 552.8 nm, surface charge of 24.3 and encapsulation efficiency of 30.74% were made. The results of the study showed that SKE:NP (50 mg/kg) reduces inflammation at all times (0, 1, 2, 3, 4 and 24 hours after subcutaneous injection). Hind paw was compared diclofenac sodium at times (0, 1, 2, 3, 4 hours after subcutaneous injection) caused a significant decrease the mice had paw inflammation, so that the lowest amount belonged to the group receiving nanogel 50 mg (0.208 g/cm3). On the other hand, the amount of paw swelling in the group receiving SKE with a dose of 100 mg/kg two hours after the applied treatment was 0.441 g/cm3 (P<0.0001). Conclusion: Comparison of the results showed that SKE:NP had more anti-inflammatory effects than SKE, which needs more investigation to explain the exact mechanism of this anti-inflammatory effect. Keywords: Hydrogel, Satureja Khuzestanica, Carvacrol, Chitosan, Formalin testReferences
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