Effects of Zataria Multiflora, Mentha Longifolia, and Origanum Vulgare Plant Essential Oils on the Inhibition of Candida Albicans

pegah shakib; Roya  Malek Hossrini; Farbod  Teymouri; Mohammad  Arjomandzadegan; Mohammad Reza  Zolfaghari*

doi:10.22087/hmj.v7i3.1023

pegah shakib Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran https://orcid.org/0000-0003-3525-226X
Roya Malek Hossrini Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran
Farbod Teymouri Department of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
Mohammad Arjomandzadegan Department of Microbiology and Immunology, School of Medicine, Infectious Diseases Research Center, Arak University of Medical Sciences, Arak, Iran
Mohammad Reza Zolfaghari* Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran

DOI: https://doi.org/10.22087/hmj.v7i3.1023

Keywords: Candida albicans, Anti-fungal, Essential oil, Minimum inhibitory concentration (MIC)

Abstract

Background and Aim: In recent years, yeast, particularly Candida species, has been the most common fungus separated from human infections. In this research, effects of essential oils of some plants on the clinical strains of Candida albicans were examined. Materials and Methods: Forty clinical strains of Candida were obtained from the Infectious Diseases Research Center of Arak University of Medical Sciences. After preparing the essential oils of Zataria multiflora, Mentha longifolia, and Origanum vulgare, the minimum inhibitory concentration (MIC) of the essential oils against Candida albicans strains was determined. the Morphological changes at different times were investigated using the negative staining method and a transmission electron microscope (TEM). Results: The results of the disc diffusion test indicated that the highest resistance rates in 0.625 mg/ml of O. vulgare, M. longifolia, and Z. multiflora essential oils were 31(77.5%), 15(37.5%), and 13(32.5%), respectively. The lowest MIC was related to Z. Multiflora essential oil (0.625 mg/ml). After treatment with the essential oils, the yeasts immediately decreased at zero hour. This decrease became more noticeable with the passage of time and reached the minimum number after 24 hours. Moreover, electron microscope images showed changes in the morphology of the yeasts. Conclusion: Zataria multiflora, Mentha longifolia, and Origanum vulgare essential oils, particularly Zataria multiflora, had antimicrobial effects against Candida albicans that were isolated to be studied. Thus, Zataria multiflora essential oil can be used as an anti-candida agent in the preparation of antifungal compounds

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