The Effect of Chenopodium Album and Apium Nodiflorum on the Expression of the Regulatory Gene (aflR) that Produces Aflatoxin in Aspergillus parasiticus

  • Fatemeh Noorbakhsh Department of Microbiolgy, Biological science collage, Islamic Azad University of Varamin- Pishva branch, Varamin- Pishva, Iran
  • Ensieh Lotfali* Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Ali Ghajari Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Saham Ansari Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Reza Mohammadi Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
  • Zahra Arab-Mazar Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medial Sciences, Tehran, Iran
Keywords: Aflatoxin, Aspergillus parasiticus, Chenopodium album, Apium nodiflorum

Abstract

Background and Aim: Aflatoxins which are mainly produced by Aspergillus parasiticus are among the highly toxic secondary metabolites. This species often contaminates food and agricultural products including cereals, peanuts, and crops in the field. Recently, a number of studies have been carried out on the elimination of aflatoxin, and the antifungal effects of medicinal herbs, such as Chenopodium album  (36HYU2385) and Apium nodiflorum (36HYU2362). In this study, the effects of C. album and A. nodiflorum as natural compounds were examined on Aspergillus parasiticus growth, aflatoxins production and the aflR gene expression.Materials and Methods: The antifungal susceptibility testing of C. album and A. nodiflorum were performed according to CLSI document M38-A2. Quantitative changes in aflR gene level of expression were analyzed by Real-time PCR method.Results: Results indicated that minimal inhibitory concentration (MIC) in the extracts of C. album and A. nodiflorum against A. parasiticus growth were 100 mg/ml and 120 mg/ml respectively. Extracts of Chenopodium album and Apium nodiflorum have antitoxic properties. Moreover, they effectively decrease aflatoxin production. The level of aflR gene expression was decreased significantly after the exposure of fungal cells to the extracts. The highest inhibition was observed in MIC= 100 mg/ml of C. album.Conclusion: According to the results, it can be suggested that these herbal extracts may have antifungal potential to be used in medicine or agriculture.

References

Carlson MP, Ensley SM. Understanding fungal (mold) toxins (mycotoxins): Cooperative Extension, Institute of Agriculture and Natural Resources, University of Nebraska-Lincoln; 2003.

Peraica M, Radic B. laucic A, Pavlovic M. Toxic Effect of Mycotoxins In Human. Bulletin of the World Health Organization. 1977;77(9):754-66.

Nazemi L, Kordbacheh P, Daei Ghazvini R, Moazeni M, Akbari Dana M, Rezaie S. Effects of thiamine on growth, aflatoxin production, and aflr gene expression in A. parasiticus. Current Medical Mycology. 2015;1(1):26-34.

Emara HA. Production of Aflatoxin by Aspergillus parasiticus and its Control. 1996.

Davari B, Khodavaisy S, Ala F. Isolation of fungi from housefly (Musca domestica L.) at Slaughter House and Hospital in Sanandaj, Iran. Journal of preventive medicine and hygiene. 2012;53(3).

Boutrif E. Prevention of aflatoxin in pistachios1. 1998.

Mohseni R, Noorbakhsh F, Moazeni M, Nasrollahi Omran A, Rezaie S. Antitoxin Characteristic of Licorice Extract: The Inhibitory Effect on Aflatoxin Production in Aspergillus parasiticus. Journal of Food Safety. 2014;34(2):119-25.

Chang P-K, Cary J, Bhatnagar D, Cleveland T, Bennett J, Linz J, et al. Cloning of the Aspergillus parasiticus apa-2 gene associated with the regulation of aflatoxin biosynthesis. Applied and Environmental Microbiology. 1993;59(10):3273-9.

Payne G, Nystrom G, Bhatnagar D, Cleveland T, Woloshuk C. Cloning of the afl-2 gene involved in aflatoxin biosynthesis from Aspergillus flavus. Applied and Environmental Microbiology. 1993;59(1):156-62.

Woloshuk C, Foutz K, Brewer J, Bhatnagar D, Cleveland T, Payne G. Molecular characterization of aflR, a regulatory locus for aflatoxin biosynthesis. Applied and Environmental Microbiology. 1994;60(7):2408-14.

Abdel-Hadi A, Carter D, Magan N. Discrimination between aflatoxigenic and non-aflatoxigenic Aspergillus section Flavi strains from Egyptian peanuts using molecular and analytical techniques. World Mycotoxin Journal. 2011;4(1):69-77.

Wang B-S, Zhao Q, Wang Y-Z. The uncertainty of assessing aflatoxin B1-producing ability using aflR gene in Aspergillus species. African Journal of Microbiology Research. 2011;5(31):5603-6.

Zargari F, Ghorbanihaghjo A, Babaei H, Farajnia S, Roodbari NH. The effect of hydroalcoholic extract of Nasturtium officinale R. Br on antioxidant status and DNA damage in liver and kidney rats exposed to arsenic. Medical Journal of Tabriz University of Medical Sciences and Health Services. 2014;36(3):44-51.

Potter JD, Steinmetz K. Vegetables, fruit and phytoestrogens as preventive agents. IARC scientific publications. 1995 (139):61-90.

Verhoeven DT, Verhagen H, Goldbohm RA, van den Brandt PA, van Poppel G. A review of mechanisms underlying anticarcinogenicity by brassica vegetables. Chemico-biological interactions. 1997;103(2):79-129.

Baldi A, Choudhary N. In vitro antioxidant and hepatoprotective potential of chenopodium album extract. International Journal of Green Pharmacy. 2013;7(1):50.

Khodavaisy S, Rezaie S, Noorbakhsh F, Baghdadi E, Sharifynia S, Aala F. Effects of Pistacia atlantica subsp. kurdica on Growth and Aflatoxin Production by Aspergillus parasiticus. Jundishapur Journal of Microbiology. 2016 (In Press).

Zaini F, Mehbod A, Emami M. Comprehensive medical mycology. Tehran University Publication Tehran Iran. 1999:39-40.

Hsieh P-C, Mau J-L, Huang S-H. Antimicrobial effect of various combinations of plant extracts. Food Microbiology. 2001;18(1):35-43.

Alma MH, Mavi A, Yildirim A, Digrak M, Hirata T. Screening chemical composition and in vitro antioxidant and antimicrobial activities of the essential oils from Origanum syriacum L. growing in Turkey. Biological and Pharmaceutical Bulletin. 2003;26(12):1725-9.

Dorman H, Deans S. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of applied microbiology. 2000;88(2):308-16.

Lone BA, Bandh SA, Chishti MZ, Bhat FA, Tak H, Nisa H. Anthelmintic and antimicrobial activity of methanolic and aqueous extracts of Euphorbia helioscopia L. Tropical animal health and production. 2013;45(3):743-9.

Bhargava A, Shukla S, Ohri D. Genetic variability and heritability of selected traits during different cuttings of vegetable Chenopodium. Indian Journal of Genetics and Plant Breeding (India). 2003.

Letelier ME, Rodríguez-Rojas C, Sánchez-Jofré S, Aracena-Parks P. Surfactant and antioxidant properties of an extract from Chenopodium quinoa Willd seed coats. Journal of Cereal Science. 2011;53(2):239-43.

Maxia A, Marongiu B, Piras A, Porcedda S, Tuveri E, Gonçalves MJ, et al. Chemical characterization and biological activity of essential oils from Daucus carota L. subsp. carota growing wild on the Mediterranean coast and on the Atlantic coast. Fitoterapia. 2009;80(1):57-61.

Tavares AC, Gonçalves MJ, Cruz MT, Cavaleiro C, Lopes MC, Canhoto J, et al. Essential oils from Distichoselinum tenuifolium: chemical composition, cytotoxicity, antifungal and anti-inflammatory properties. Journal of ethnopharmacology. 2010;130(3):593-8.

Maxia A, Frau MA, Marongiu B, Piras A, Porcedda S, Falconieri D, et al. Biological assays and chemical composition of volatile oils of Bupleurum fruticosum L.(Apiaceae). Academy of Chemistry of Globe Publications. 2011;2:92-9.

Bagheri-Gavkosh S, Bigdeli M, Shams-Ghahfarokhi M, Razzaghi-Abyaneh M. Inhibitory effects of Ephedra major host on Aspergillus parasiticus growth and aflatoxin production. Mycopathologia. 2009;168(5):249-55.

Maxia A, Falconieri D, Piras A, Porcedda S, Marongiu B, Frau MA, et al. Chemical composition and antifungal activity of essential oils and supercritical CO2 extracts of Apium nodiflorum (L.) Lag. Mycopathologia. 2012;174(1):61-7.

Kumar R, Mishra AK, Dubey N, Tripathi Y. Evaluation of Chenopodium ambrosioides oil as a potential source of antifungal, antiaflatoxigenic and antioxidant activity. International Journal of Food Microbiology. 2007;115(2):159-64.

Gawlik-Dziki U, Świeca M, Sułkowski M, Dziki D, Baraniak B, Czyż J. Antioxidant and anticancer activities of Chenopodium quinoa leaves extracts–in vitro study. Food and Chemical Toxicology. 2013;57:154-60.

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