The Effect of Rosmarinus officinalis L Extract on the Inhibition of High Glucose-Induced Neurotoxicity in PC12 Cells: an In Vitro Model of Diabetic Neuropathy

  • Marzieh Rashidipour Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Zahra Hajializadeh Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran
  • Saeed Esmaeili-Mahani Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
  • Ayat Kaeidi * Physiology-pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran Department of Physiology and Pharmacology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Keywords: Rosmarinus officinalis, hyperglycemia, cell toxicity, apoptosis, diabetic neuropathy, PC12 cells


Background and Aim: Diabetes mellitus and continued hyperglycemic condition prompt serious diabetic complications such as diabetic neuropathy. Numerous studies have shown the involvement of oxidative stress and high glucose-induced cell death in the progress of diabetic neuropathy. Rosmarinus officinalis L has been suggested in the literature as an anti-diabetic herbal medicine that contains potent antioxidant components. Therefore, the neuroprotective effect of Rosmarinus officinalis L (RE) extract was investigated in glucose-induced neurotoxicity via pheochromocytoma )PC12( cells as an appropriate in vitro model of diabetic neuropathy.Materials and Methods: Cell viability was determined using MTT assay. Cleave caspase-3 and Bax: Bcl2 ratio, as biochemical parameters of cellular apoptosis, were measured by western blotting analysis.Results: Our data showed that a 4-fold elevation in the medium glucose significantly reduced cell viability (P < 0.01) and increased caspase-3 activation (P < 0.01) as well as Bax: Bcl2 ratio (P < 0.05) in PC12 cells after 24 h. Incubation of high glucose medium cells with 60μg/ml RE extract decreased high glucose-induced cell toxicity and prevented caspase-3 activation and Bax: Bcl2 ratio.Conclusion: It could be concluded that RE extract is effective in the protection against hyperglycemia-induced cellular toxicity. This could be relevant to the prevention of apoptosis. Moreover, the results suggest that RE has the therapeutic potential to attenuate diabetes complications such as that neuropathy.


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Original Article