Phytochemical Screening, Antioxidant and Gastro-Protective Activity Studies on the Fruit Peels of Selected Varieties of Banana

  • Dennis Gogola * Faculty, Nursing Department, Bicol University Tabaco Campus, Albay, the Philippines
Keywords: Gastroprotective, Anti-ulcer, Antioxidant, Phytochemicals, Banana peels


Background and Aim: Gastric ulcer is a chronic health condition of the gastrointestinal tract with increasing incidence and prevalence affecting both male and female population globally. There are a variety of drugs used in the treatment of this disease, but they are costly and exhibit limited efficacy, and cause adverse reactions. Hence, the present study aimed to screen the phytochemicals, antioxidant and anti-ulcer activities of the ethanolic fruit peel extracts of M. acuminata Colla, M. paradisiaca L., and M. acuminata Colla cv. Señorita. Materials and Methods: The determination of total phenolics and flavonoids were performed using conventional methods. The free radical scavenging activity of the extracts was investigated using the DPPH (1, 1-Diphenyl-2 -picrylhydrazyl). Moreover, the anti-ulcer activity was examined using three ulcer models in rats. Results: The total phenolic contents of the peel extracts ranged from 12.3 to 40.3 mg gallic acid equivalents (GAE)/g dry weight (dw), whereas the flavonoid content ranged from 2.5 to 16.6 mg GAE/g dw. The results indicated that the peel extracts exhibited free radical scavenging capacity (DPPH) that ranged from 51.52 to 72.00% at the concentration of 100 μg/mL. Moreover, the peel extracts showed gastroprotective activities against alcohol, drug, and stress-induced ulcers in rats. The maximum percentage of ulcer inhibition of the peel extracts against ethanol-induced ulcer was 83.33, whereas it was 100% against both aspirin and cold restraint stress-induced ulcers. Conclusion: The obtained results suggest that the fruit peel extracts of M. acuminata Colla, M. paradisiaca L., and M. acuminata Colla cv. Señorita have antioxidant capacities. They also exhibited anti-ulcer activities, and contained active compounds that are potentially responsible for its biological and pharmacological properties. Likewise, further explorations of the peel extracts are required, for the plant examined in this research is a promising plant potentially applicable in nutraceutical and pharmaceutical industries.


Laloo D, Prasad SK, Krishnamurthy S, Hemalatha S. Gastroprotective activity of ethanolic root extract of Potentilla fulgens Wall. ex Hook. Journal of ethnopharmacology. 2013;146(2):505-14.

Shristi B, Neha J, Indu BP, Rajesh G. A review on some Indian medicinal plants for antiulcer activity. J Sci Res Pharm. 2012;1:6-9.

Saheed S, Oladipo AE, Sunmonu TO, Balogun FO, Ashafa AO. The Purview of Phytotherapy in the Management of Gastric Ulcer. Stomach Disorders. 2018;23.

Paula de Oliveira A, Santin JR, Lemos M, Klein Júnior LC, Couto AG, Meyre da Silva Bittencourt C, Filho VC, Faloni de Andrade S. Gastroprotective activity of methanol extract and marrubiin obtained from leaves of Marrubium vulgare L.(Lamiaceae). Journal of Pharmacy and Pharmacology. 2011;63(9):1230-7.

Barocelli E, Chiavarini M, Ballabeni V, Barlocco D, Vianello P, Dal Piaz V, Impicciatore M. Study of the antisecretory and antiulcer mechanisms of a new indenopirydazinone derivative in rats. Pharmacological research. 1997;35(5):487-92.

Shadma A, Sundaram S, Rai GK. Nutraceutical application and value addition of banana peel: A review. Int J Pharm Pharm Sci. 2014;6(10):81-5.

Kapadia SP, Pudakalkatti PS, Shivanaikar S. Detection of antimicrobial activity of banana peel (Musa paradisiaca L.) on Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans: An in vitro study. Contemporary clinical dentistry. 2015;6(4):496.

Vu HT, Scarlett CJ, Vuong QV. Phenolic compounds within banana peel and their potential uses: A review. Journal of Functional Foods. 2018;40:238-48.

Imam MZ, Akter S. Musa paradisiaca L. and Musa sapientum L.: A phytochemical and pharmacological review. Journal of Applied Pharmaceutical Science. 2011;1(5):14-20.

González-Montelongo R, Lobo MG, González M. Antioxidant activity in banana peel extracts: Testing extraction conditions and related bioactive compounds. Food Chemistry. 2010;119(3):1030-9.

De Castro ML, Garcıa-Ayuso LE. Soxhlet extraction of solid materials: an outdated technique with a promising innovative future. Analytica chimica acta. 1998;369(1-2):1-0.

Wang L, Weller CL. Recent advances in extraction of nutraceuticals from plants. Trends in Food Science & Technology. 2006;17(6):300-12.

Hossain MD, Sarwar MS, Dewan SM, Hossain MS, Shahid-Ud-Daula AF, Islam MS. Investigation of total phenolic content and antioxidant activities of Azadirachta indica roots. Avicenna journal of phytomedicine. 2014;4(2):97.

Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of food and drug analysis. 2002;10(3).

Baba SA, Malik SA. Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume. Journal of Taibah University for Science. 2015;9(4):449-54.

Hollander D, Tarnawski A, Krause WJ, Gergely H. Protective effect of sucralfate against alcohol-induced gastric mucosal injury in the rat: macroscopic, histologic, ultrastructural, and functional time sequence analysis. Gastroenterology. 1985;88(1):366-74.

Rao CV, Ojha SK, Radhakrishnan K, Govindarajan R, Rastogi S, Mehrotra S, et al. Antiulcer activity of Utleria salicifolia rhizome extract. Journal of Ethnopharmacology. 2004;91(2-3):243-9.

Sairam KC, Rao CV, Babu MD, Kumar KV, Agrawal VK, Goel RK. Antiulcerogenic effect of methanolic extract of Emblica officinalis: an experimental study. Journal of Ethnopharmacology. 2002;82(1):1-9.

Petti S, Scully C. Polyphenols, oral health and disease: A review. Journal of dentistry. 2009;37(6):413-23.

Song FL, Gan RY, Zhang Y, Xiao Q, Kuang L, Li HB. Total phenolic contents and antioxidant capacities of selected Chinese medicinal plants. International Journal of Molecular Sciences. 2010;11(6):2362-72.

Harborne JB. General procedures and measurement of total phenolics. Methods in plant biochemistry. 1989;1:1-28.

Bravo L. Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutrition reviews. 1998;56(11):317-33.

González-Montelongo R, Lobo MG, González M. Antioxidant activity in banana peel extracts: Testing extraction conditions and related bioactive compounds. Food Chemistry. 2010;119(3):1030-9.

Morais DR, Rotta EM, Sargi SC, Schmidt EM, Bonafe EG, Eberlin MN, et al. Antioxidant activity, phenolics and UPLC–ESI (–)–MS of extracts from different tropical fruits parts and processed peels. Food Research International. 2015;77:392-9.

Benavente-Garcıa O, Castillo J, Lorente J, Ortuno A, Del Rio JA. Antioxidant activity of phenolics extracted from Olea europaea L. leaves. Food chemistry. 2000;68(4):457-62.

Manach C, Mazur A, Scalbert A. Polyphenols and prevention of cardiovascular diseases. Current opinion in lipidology. 2005;16(1):77-84.

Middleton E, Kandaswami C, Theoharides TC. The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacological reviews. 2000;52(4):673-751.

Samman S, Lyons Wall PM, Cook NC. Flavonoids and coronary heart disease: Dietary perspectives.

Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. The Journal of nutritional biochemistry. 2002;13(10):572-84.

Baxter H, Harborne JB, Moss GP, editors. Phytochemical dictionary: a handbook of bioactive compounds from plants. CRC press; 1998 Dec 1.

Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free radical biology and medicine. 1996;20(7):933-56.

Iwashina T. The structure and distribution of the flavonoids in plants. Journal of Plant Research. 2000;113(3):287-99.

Xiao K, Xuan L, Xu Y, Bai D, Zhong D, Wu H, et al. Dimeric stilbene glycosides from Polygonum cuspidatum. European Journal of Organic Chemistry. 2002;2002(3):564-8.

Cushnie TT, Lamb AJ. Antimicrobial activity of flavonoids. International journal of antimicrobial agents. 2005;26(5):343-56.

Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: an overview. The Scientific World Journal. 2013;2013.

Tripoli E, La Guardia M, Giammanco S, Di Majo D, Giammanco M. Citrus flavonoids: Molecular structure, biological activity and nutritional properties: A review. Food chemistry. 2007;104(2):466-79.

Tapas AR, Sakarkar DM, Kakde RB. Flavonoids as nutraceuticals: a review. Tropical Journal of Pharmaceutical Research. 2008;7(3):1089-99.

Conde-Hernández LA, Guerrero-Beltrán JÁ. Total phenolics and antioxidant activity of Piper auritum and Porophyllum ruderale. Food chemistry. 2014;142:455-60.

Auddy B, Ferreira M, Blasina F, Lafon L, Arredondo F, Dajas F, et al. Screening of antioxidant activity of three Indian medicinal plants, traditionally used for the management of neurodegenerative diseases. Journal of Ethnopharmacology. 2003;84(2-3):131-8.

Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;181(4617):1199.

Oki T, Masuda M, Furuta S, Nishiba Y, Terahara N, Suda I. Involvement of anthocyanins and other phenolic compounds in radical‐scavenging activity of purple‐fleshed sweet potato cultivars. Journal of Food Science. 2002;67(5):1752-6.

Ravichandran K, Ahmed AR, Knorr D, Smetanska I. The effect of different processing methods on phenolic acid content and antioxidant activity of red beet. Food Research International. 2012;48(1):16-20.

Tucker GA, Seymour GB, Taylor JE, Tucker GA. Biochemistry of fruit ripening.

Kanazawa K, Sakakibara H. High content of dopamine, a strong antioxidant, in cavendish banana. Journal of agricultural and food chemistry. 2000;48(3):844-8.

Nguyen QV, Eun JB. Antioxidant activity of solvent extracts from Vietnamese medicinal plants. Journal of Medicinal Plants Research. 2011;5(13):2798-811.

Surveswaran S, Cai YZ, Corke H, Sun M. Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants. Food chemistry. 2007;102(3):938-53.

Akhtar N, Mirza B. Phytochemical analysis and comprehensive evaluation of antimicrobial and antioxidant properties of 61 medicinal plant species. Arabian journal of chemistry. 2015.

Wasman SQ, Mahmood AA, Salehhuddin H, Zahra AA, Salmah I. Cytoprotective activities of Polygonum minus aqueous leaf extract on ethanol-induced gastric ulcer in rats. Journal of Medicinal Plants Research. 2010;4(24):2658-65.

Ghasi S. Evaluation of the anti-ulcer property of aqueous extract of unripe Musa paradisiaca Linn. peel in Wistar rats. African Journal of Pharmacy and Pharmacology. 2014;8(39):1006-11.

Ikpeazu O, Elekwa I, Ugbogu A, Arunsi U, Uche-Ikonne C. Preliminary evaluation of anti-ulcer potential of aqueous extract of fermented unripe Musa paradisiaca in Wistar Rats. Am J Biomed Res. 2017;5:17-23.

Mbagwu H, Jackson C, Ekpo M, Okopedi E, Anah V, Ugwu C. Gastroprotective effects of ethanolic leaf extract of Musa paradisiaca L.(Musaceae) in rats. J. Chem. Pharm. Res. 2011;3(3):322-7.

Nagaraju B, Anand SC, Ahmed N, Chandra JN, Ahmed F, Padmavathi GV. Antiulcer activity of aqueous extract of Citrus medica Linn. fruit against ethanol induced ulcer in rats. Advances in Biological Research. 2012;6(1):24-9.

Wallace JL. How do NSAIDs cause ulcer disease?. Best Practice & Research Clinical Gastroenterology. 2000;14(1):147-59.

Biswas K, Bandyopadhyay U, Chattopadhyay I, Varadaraj A, Ali E, Banerjee RK. A novel antioxidant and antiapoptotic role of omeprazole to block gastric ulcer through scavenging of hydroxyl radical. Journal of Biological Chemistry. 2003;278(13):10993-1001.

Govindarajan R, Vijayakumar M, Singh M, Rao CV, Shirwaikar A, Rawat AK, et al. Antiulcer and antimicrobial activity of Anogeissus latifolia. Journal of Ethnopharmacology. 2006;106(1):57-61.

Cho CH, Ogle CW, Dai S. Acute gastric ulcer formation in response to electrical vagal stimulation in rats. European journal of pharmacology. 1976;35(1):215-9.

Cho CH, Ogle CW. Cholinergic-mediated gastric mast cell degranulation with subsequent histamine H1-and H2-receptor activation in stress ulceration in rats. European journal of pharmacology. 1979;55(1):23-33.

Hase T, Moss BJ. Microvascular changes of gastric mucosa in the development of stress ulcer in rats. Gastroenterology. 1973;65(2):224-34.

Maiti RN, Goel RK. Effect of mild irritant on gastric mucosal offensive and defensive factors. Indian J Physiol Pharmacol. 1999;44(2):185-91.

De Barros MP, Lemos M, Maistro EL, Leite MF, Sousa JP, Bastos JK, et al. Evaluation of antiulcer activity of the main phenolic acids found in Brazilian Green Propolis. Journal of ethnopharmacology. 2008;120(3):372-7.

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