In Vitro Antibacterial, Anticoagulant, and Antioxidant Screening of Aqueous Extracts of Blue Ternate (Clitoria ternatea L.) Flower

  • Roque Merrick Dacullo Department of Biological Sciences, College of Arts and Sciences, Visayas State University, Visca, Baybay City 6521, Leyte, Philippines
  • Jayzon Gaspay Bitacura * Department of Biological Sciences, College of Arts and Sciences, Visayas State University, Visca, Baybay City 6521, Leyte, Philippines
Keywords: Antibacterial agents, Anticoagulants, Antioxidants, Decoction, Homogenate, Leachate and Tea, Flower extracts


Background and Aim: Extracts of Clitoria ternatea flowers obtained through laboratory standard methods have been constantly reported to have various medicinal activities. However, no reports on such properties are available for this flower’s extracts obtained through common domestic extraction procedures. This study aimed to determine and compare the aqueous extracts (leachate, decoction, tea, and homogenate) of C. ternatea flowers for their antibacterial, anticoagulant, and antioxidant activities. Materials and Methods: Agar well diffusion and Lee-White test tube method were used for antibacterial and anticoagulant assays, respectively. Moreover, enzymatic and non-enzymatic antioxidant assays of the extracts were conducted through the floating disc assay, and DPPH scavenging activity, respectively. Results: The results of the present study revealed that although the extracts exhibited antibacterial activities against Salmonella enterica and Staphylococcus aureus, they did not differ significantly at p>0.05. The extracts did not exhibit in vitro anticoagulant activity. Only the homogenate preparations showed catalase activity while all the extracts showed DPPH scavenging activity with the flower decoction as the highest % DPPH RSA harmonic mean of 56.24%, followed by the leachate (45.03%), and the tea (38.79%) and homogenate (37.74%) as the least. Conclusion: C. ternatea flowers extracted through common domestic extraction methods were revealed to have antibacterial, procoagulant, and antioxidant properties. The extracts could also be subjected to phytochemical screening and could be studied for their other bioactive properties.


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Original Article