Targeting and Inhibiting Plasmodium berghei Growth in Balb/c Mice Using Kojic Acid-Solid Lipid Nanoparticles and Kojic Acid-Nanostructured Lipid Carriers

Mehdi Nateghpour*; Aref Faryabi; Afsaneh Motevalli Haghi*; Khadijeh Khezri; Bahman  Rahimi-Esboei; Abbas Rahimi Foroushani; Nader Shahrokhi; Amir Amani; Fatemeh   Bayat

doi:10.22087/hmj.v7i3.991

Mehdi Nateghpour* Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Aref Faryabi Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences (TUMS), Tehran, Iran
Afsaneh Motevalli Haghi* Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences (TUMS), Tehran, Iran
Khadijeh Khezri Department of Nursing, Khoy University of Medical Sciences, Khoy, Iran. Neurophysiology Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran
Bahman Rahimi-Esboei Department of Medical Parasitology and Mycology, School of medicine, Islamic Azad University, Tonekabon branch, Iran
Abbas Rahimi Foroushani Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Nader Shahrokhi Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
Amir Amani Department of Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
Fatemeh Bayat Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences (TUMS), Tehran, Iran

DOI: https://doi.org/10.22087/hmj.v7i3.991

Keywords: Malaria, Plasmodium berghei, Kojic acid, Nanoparticles, Nanostructure

Abstract

Background and Aim: Malaria is a life-threatening infection in the world. The emergence of strains of Plasmodium species that are resistant to anti-parasitic drugs, and the lack of licensed high-performance malaria vaccines have raised serious concerns worldwide. In recent years, new treatment strategies such as nanoformulations have been suggested as effective drug delivery systems to enhance the therapeutic efficiency of various drugs. Materials and Methods: In this study, kojic acid-solid lipid nanoparticles (KA-SLNs) and kojic acid-nanostructured lipid carriers (KA-NLCs) were synthesized using high-speed homogenization and ultra-probe sonication methods to improve their antiplasmodial activities. The obtained nanoformulations were evaluated against the Plasmodium berghei malaria parasite in mice. Anti-plasmodium activities and cytotoxicity of the nanoparticles were assed. Furthermore, the spleen and liver biochemical analyses of the subjected mice were evaluated for each group of mice in comparison with the control group. Fifty percent effective dose (ED50) was calculated as well. Moreover, ex vivo human red blood cells (RBCs) hemolysis was assessed. Results: Kojic acid solution was significantly effective in all concentrations on the seventh day (D7) and the tenth day (D10) (P. value <0.05). The toxicity test revealed no toxic impact on the subjects. ED50 was obtained at 150 mg/kg concentration for KA-NLCs and 400 mg/kg concentration for KA-SLNs on D10. The results of the evaluation of KA nanoformulations and KA solution on RBCs indicated that KA nanoformulations could reduce the lysis of RBCs. These results also showed that the lysis of RBCs increased with raising drug concentration in KA nanoformulations, and KA-NLCs (100 mg/kg) gave the least lysis. KA nanoformulations (especially KA-NLCs) and KA solution significantly reduced parasite growth. Conclusion: These results revealed that the KA solution was safe and had no side effects on the subjects in the range of evaluated concentrations. Moreover, the results of this study showed that

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