The Effect of the Hydroalcoholic Extract of Quercus infectoria Fruit Hulls (Jaft-E-Baloot) on Formalin-Induced Inflammation and Pain in Male Mice

  • Ali Karimian Student Research Committee, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Amirhossein Nafari Student Research Committee, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Amirmehdi Sarvestani Student Research Committee, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Amir Zamani Student Research Committee, School of Dentistry, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  • Nima Behnaminia Student Research Committee, Kashan University of Medical Sciences, Kashan, Iran
  • Rasool Mohammadi Razi Herbal Medicines Research Center,School of Health and Nutrition, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Mohammad Almasian School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
  • Ali Asghar Kiani* Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran
Keywords: Analgesic, Anti-inflammatory, Quercus infectoria


Background and Aim: Herbal medicines have been used to alleviate inflammation and pain since ancient times, and their use is even on the rise. The Quercus infectoria plant has been commonly used for many years to treat inflammation and pain in alternative medicine. Various species and different parts of this plant have been studied. The present study aimed to investigate the anti-inflammatory and analgesic properties of the extract of the fruit hulls of Q. infectoria to find a plant-based alternative to the available drugs with fewer side effects. Materials and Methods: This study used 60 male NMRI mice with a weight range of 35-40 g. The mice were randomized into a negative control group which only received formalin, a positive control group that received diclofenac 200 µg/kg, and four experimental groups that received 50, 100, 150, and 200 mg/kg of the fruit hulls extraction. Formalin was injected into the paws of the mice to induce inflammation and pain. Then, the paw volume was measured during the first three hours after injection with a digital Plethysmometer. Pain score was also evaluated in three stages at 60-minute intervals. Results: The results showed that the fruit hulls extract could reduce inflammation at all doses, particularly at 200 mg/kg in comparison with the negative control group (p < 0.001). Moreover, the fruit hulls extract relieved pain at different doses in acute and chronic stages. Conclusion: The fruit hulls extract alleviated the pain and reduced the inflammation in mices' paws depending on the dose. Therefore, it can be considered as a possible alternative to chemical drugs.


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