Pretreatment with Olive Leaf Extract Improves Renal and Liver Antioxidant Systems Following Renal Ischemia-Reperfusion Injury in Rats
Abstract
Background and Aim: The effect of Olive leaf extract (OLE) as a natural antioxidant was investigated on renal and liver antioxidant enzyme activities, after renal ischemia-reperfusion (IR) in a rat model.Materials and Methods: Forty male rats divided into 5 groups: Sham (no IR), 3 "OLE+IR" groups (25mg/kg, 50mg/kg and 100mg/kg oral OLE once daily for 2 weeks, prior to renal IR) and "water+IR" group (oral gavage of water prior to IR). In the last 4 groups, both renal pedicles clamped for 45 minutes followed by 24 hours of reperfusion. Clearance of creatinine was measured. Renal and liver catalase and glutathione peroxidase activities and glutathione level were assessed as well. Volume densities of the renal proximal convoluted tubules were measured stereologicaly.Results: Glutathione level, catalase and glutathione peroxidase activities, clearance of creatinine and volume density of the proximal convoluted tubules decreased in "water+IR" compared to the sham group. Pretreatment with OLE in 3 different doses significantly increased renal antioxidant enzymes, creatinine clearance and volume density of the proximal convoluted tubules in comparison with "water+IR" group. Pretreatment with only one dose (25mg/kg) of OLE significantly increased liver glutathione and GPx compared to the "water+IR" group. Pretreatment with 2 doses (25mg/kg, 50mg/kg) increase liver catalase compared to the water+IR group.Conclusions: Two weeks of oral pretreatment with different doses of OLE decreased renal injury couesd by renal IR. This was associated with increased renal catalase and glutathione peroxidase activities. Some doses of OLE were also associated with increased liver antioxidant system parametes.References
Brady He, Singer Gg. Acute renal failure. Lancet. 1995;346:1533-40.
Ellenberger C, Schweizer A, Diaper J, et al.Incidence, risk factors and prognosis of changes in serum creatinine early after aortic abdominal surgery. Intens Care Med, 2006;32(11):1808-16.
Thadhani R, Pascual M, Bonventre JV. Acute renal failure. N Engle J Med. 1996;334:1448-60
Noiri E, Nakao A, Uchida K, et al. Oxidative and nitrosative stress in acute renal schemia. Am J Phsiol Renal Phsiol. 2001;281:F948-57.
Hagiwara S, Koga H, Iwasaka H, et al. ETS-GS, a new antioxidant, ameliorates renal ischemia reperfusion injury in a rodent model. Journal of Surgical Research. 2011;171:226-33
Polat C, Tokyo l, Kahraman A, Sabuncuoglu B,Ylmaz S. The effects of desferrioxamine and quercetin on hepatic ischemia-reperfusion induced renal disturbance. Prostaglandins Leukot and Essent Fatty Acids. 2006;74(6):379-83.
Rajan D, Wu R, Shah KG, Jacob A, Coppa GF, Wang P. Human ghrelin protects animals from renal ischemia-reperfusion injury through the vagus nerve. Surgery. 2012;151:37-47.
Melin J, Hellberg O, Akyurek LM, Kallskog O, Larsson E, Fellstrom BC. Ischemia causes rapidly progassive nephropathy in diabetic rat. Kidney Int . 1997; 52:985-91.
Yildirim A, Gumus M, Dalga S, Sahin YN, Akcay F. Dehydroefiand roster one improves hepatic antioxidant systems after renal ischemia reperfusion injury in rabits. Ann Clin Lab Sci. 2003;33:459-64.
Serteser M, Koken T, Kahraman A, Yilmaz K, Akbulut G, Dilek ON. Change in hepatic TNF-α levels. Antioxidant status and oxidation product after renal ischemia-reperfusion injury in mice. Journal of Surgery Research. 2002;107:234-40.
Karthikumar S, Vigneswari K, Jegatheesan K. Screening of antibacterial and antioxidant activities of leaves of Eclipta prostrata (L). Scientific Research and Essay. 2007;2 (4):101-4.
Lee-Huang S, Zhang L, Lin Huang P, Chang Y, Huang P. Anti-HIV activity of infection and OLE treatment. Biochemical and Biophysical Research Communications. 2003;307(4):1029-37.
Pereira A, Ferreira I, Marcelino F, et al. Phenolic compounds and antimicrobial activity of olive (Olea europaea L. Cv. Cobrançosa) leaves. Molecules. 2007;12(5):1153-62.
Susalit E, Agus N, Effendi I, et al. Olive (Olea europaea) leaf extract effective in patients with stage-1 hypertension: Comparison with Captopril. Phytomedicine. 2011;18:251–8.
Wainstein J, Ganz T, Boaz M, et al. Olive Leaf Extract as a Hypoglycemic Agent in Both Human Diabetic Subjects and in Rats. Journal of Medicinal Food. 2012;15(7):1-6.
Rafiei Z, Jafari SM, Alami M, Khomeiri M. Antioxidant Properties of Olive Leaf Extract and its Application in Sunflower Oil . Research Journal of Food. 2011; 21(1) : 11-23.
Mashiach E, Sela S, Weinstein T, et al. Mesna: A novel renoprotective antioxidant in ischemic acute renal failure. Nephrol Dial Transplant. 2001;16(3):542-551.
Korkmaz A, Kolankaya D. The protective effects of ascorbic acid against renal ischemia reperfusion injury in male rats. Ren Fail. 2009;31(1):36-43.
Mohagheghi F, Bigdeli MR, Rasoulian B, Hashemi P, Rashidi Pour M. The neuro protective effect of olive leaf extract is related to improved blood –brain barrier permeability and brain edema in rat with experimental focal cerebral ischemia. Phytomedicine. 2011;18:170-5.
Wahhabaghai H, Heidari R, Zeinoddini A, Soleyman-Jahi S, Golmanesh L, Rasoulian B, et al. Hyperoxia-induced preconditioning against renal ischemic injury is mediated by reactive oxygen species but not related to heat shock proteins 70 and 32. Surgery. 2015;157(6):1014-22.
Rajan D, Wu R, Shah KG, et al. Human ghrelin protects animals from renal ischemia-reperfusion injury through the vagus nerve. Surgery. 2012;151:37-47.
Rungby J, Ernst E. Experimentally induced lipid peroxidation after exposure to chromium, mercury or silver: Interactions with carbon tetrachloride. Pharmacol Toxicol. 1992;70: 205.
Sedlak J, Lindsay HR. Estimation of total protein and nonprotein sulfhydryl groups in tissue with Ellmans reagent. Anal Biochem. 1968;25: 192.
Hfaieh N, Mbarki S, Alimi H, Murat JC, Elfeki A. Diabetes-induced damages in rat kidney and brain and protective of natural antioxidants.Food and Nutrition Sciences. 2013;4:436-44.
Flohe L, Gunzler WA. Assays of glutathione peroxidase. Methode in Enzymology. 1984;105:114-21.
Carlos A. Stereological tools in biochemical research. Acad Bras Cienc. 2003;75:413.
Cicora F, Lausada N, Vasquez DN, et al. Sirolimus in kidney transplant donors and clinical and histologic improvement in recipients: rat model. Transplantation Proceedings. 2010;42:365-70.
Sadeghnia HR, Boroushaki MT, Mofidpour H. Effect of safranal on lipid peroxidation level during renal ischemia-reperfusion injury in rat. Iran J Basic Med Sci. 2005;8: 179-85.
Korkmaz A, Kolankaya D. Protective effect of rutin on the ischemia/reperfusion induced damage in rat kidny. Journal of Surgical Research. 2010;164:309-315.
Koga H, Hagiwara S, Hideto M, et al. The vitamin E derivative, ESeroS-GS, attenuates renal ischemia-reperfusion injury in rats. Journal of Surgical Research. 2011;1-6.
Baud L, Ardaillou R. Involvement of reactive oxygen species in kidney damage. Br Med Bull. 1993;49:621.
Tavafi M, Ahmadvand H, Toolabi P. Inhibitory effect of olive leaf extract on gentamicin-induced nephrotoxicity in rat. Iranian Journal Kidney Dissease. 2012;6:25-32.
Jemai H, Bouaziz M, Fki I, El Feki A, Sayadi S. Hypolipidimic and antioxidant activities of oleuropein and its hydrolysis derivative rich extracts from chemlali olive leaves. Chem. Biol. Intract. 2008;176:88-98.
Beiranvand A, Rasoulian B, Alirezaei M, et al. Pretreatment with olive leaf Extract partially attenuates cisplatin-induced nephrotoxicity in rat. Yafteh. 2010;11(5):35-44.
Hu H, Batteux F, Chereau C, et al. Clopidogrel protects from cell apoptosis and oxidative damage in a mouse model of renal ischemia-reperfusion injury. Journal of Pathology. 2011;225:265-75.
Rasoulian B, Jafari M, Noroozzadeh A, et al. Effects of Ischemia-Reperfusion on rat renal tissue antioxidant systems and lipid peroxidation. Acta Medica Iranica. 2008;46(5):353-60. 37. Emre MH, Erdogan H, Fadillioglu E. Effect of BQ-123 and Nitric Oxide Inhibition on Liver in Rats after Renal Ischemia-Reperfusion Injury. Gen Physiol Biophys. 2006;25:195–206.
Yildirim A, Gumus M, Dalga S, Sahin YN, Akcay F. Dehydroepiandrosterone Improves Hepatic Antioxidant Systems after Renal Ischemia-Reperfusion Injury in Rabbits. Annals of Clinical & Labora- tory Science. 2003;33(4):459-64.
Sezgin G, Ozturk G, Guney S, Sinanoglu O, Tuncdemir M. Protective effect of melatonine and 1,25- Dihydroxyvitamine D3 on renal Ischemia-Reperfusion injury in rats. Renal Failure. 2013;35(3):374-9.
Seth P, Kumari R, Madhavan S, et al. Prevention of renal ischemia-reperfusion-induced injury in rats by picroliv. Biochemical Pharmacology. 2000; 59:1315-22.
Rabiei Z, Bigdeli MR, Rasoulian B, Ghassempour A, Mirzajani F. The neuroprotection effect of pretreatment with olive leaf extract on brain lipidomics in rat stroke model. Phytomedicine. 2012;19(10):940-6.
Mohagheghi F, Bigdeli M, Rasoulian B, Asghar Zeinanloo A. Effect of dietary virgin olive oil on serum lipids levels in rats. Physiol Pharmacol. 2010;13(4):379-85.
Esmailidehaj M, Rasoulian B, Rezvani ME, Delfan B, Mosaddeghmehrjardi MH, Pourkhalili Kh. The anti-infarct, antistunning and antiarrhythmic effects of oleuropein in isolated rat heart. EXCLI Journal. 2012;11:150-62.
Zare L, Esmaeili-Mahani S, Abbasnejad M, et al.Oleuropein, chief constituent of olive leaf extract, prevents the development of morphine antinociceptive tolerance through inhibition of morphine-induced L-type calcium channel overexpression. Phytotherapy Research. 2011;26(11):1731-7.
Esmailidehaj M, Mirhosseini SJ, Rezvani ME, Rasoulian B, Mosaddeghmehrjardi MH, Haghshenas D. Prolonged Oral Administration of Oleuropein Might Protect Heart against Aconitine-induced Arrhythmia. Iran Journal of Pharm Research. 2012;11(4);1255-1263.
Khalatbary AR, Ahmadvand H. Neuroprotective effect of oleuropein following spinal cord injury in rats.Neurological Research. 2012;34(1):44-51.
Alirezaei M, Dezfoulian O, Neamati S, Rashidipour M, Tanideh N, Kheradmand A.Oleuropein prevents ethanol-induced gastric ulcers via elevation of antioxidant enzyme activities in rats. Journal of Physiology and Biochemistry. 2012;68(4):583-92.
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