Bioassay Screening of the Essential Oil and Various Extracts of Nigella sativa L. Seeds Using Brine Shrimp Toxicity Assay

Fariba Sharififar, Ali Assadipour, Mohammad Hassan Moshafi, Fahimeh Alishahi, Hossein Mahmoudvand*

Abstract


Background and Aim: Since cytotoxicity screening is the first step necessary for any new drug development, this study was designed to find out and compare the cytotoxicity effects of the essential oil and various extracts of Nigella sativa L. seeds using Brine Shrimp Lethality (BSL) assay.

Materials and Methods: Essential oils and various extracts of N. sativa were assessed by two methods of disk and solution of BSL. Data analysis was carried out using SPSS statistical package version 17.0 (SPSS Inc., Chicago, IL, 250 USA). Data were processed in probit-analysis program to estimate LC50 values.

Results: All of the tested fractions demonstrated more cytotoxicity in the solution method. Petroleum ether and chloroform extract of N. sativa showed the most cytotoxicity with LC50 values 7 and 21 μg/ml respectively; while aqueous and ethanolic had no significant cytotoxicity. Moreover, the GC/MS analysis of the essential oil of N. sativa showed the p-cymene (48.1%), α-thujone (14.38%) and dihydro carveol (9.11%) as the main compounds.

Conclusion: These results suggest some limitation for using this spice in diet. Furthermore, this plant could be considered as a source of cytotoxic compounds which should be studied in details.


Keywords


Nigella sativa, Black cumin, Artemia salina, Cytotoxicity, Essential oil

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References


Rocha LG, Almeida JR, Macedo RO, Barbosa-Filho JM. A review of natural products with antileishmanial activity. Phytomed. 2005;12:514-35.

Ali B, Blunden G. Pharmacological and toxicological properties of Nigella sativa. Phytother Res. 2009;17:299–305.

Randhawa MA, Al-Ghamdi MS. A review of the pharmacotherapeutic effects of Nigella sativa. Pak J Med Res. 2002;41:77–83.

Mahmoudvand H, Dezaki ES, Kheirandish F, Ezatpour B, Jahanbakhsh S, Harandi MF. Scolicidal effects of black cumin seed (Nigella sativa) essential oil on hydatid cysts. Korean J Parasitol. 2014;52(6):653-9.

Mahmoudvand H, Sepahvand A, Jahanbakhsh S, Ezatpour B, Mousavi SA. Evaluation of antifungal activities of the essential oil and various extracts of Nigella sativa and its main component, thymoquinone against pathogenic dermatophyte strains. J Med Mycol. 2014;24(4):155-61

Mahmoudvand H, Tavakoli R, Sharififar F, Minaie K, Ezatpour B, Jahanbakhsh S, Sharifi I. Leishmanicidal and cytotoxic activities of Nigella sativa and its active principle, thymoquinone. Pharm Biol. 2014;4(0):1-6.

Mahmoudvand H, Asadi A, Harandi MF, Sharififar F, Jahanbakhsh S, Dezaki ES. In vitro lethal effects of various extracts of Nigella sativa seed on hydatid cyst protoscoleces. Iran J Basic Med Sci. 2014;17(12):1001-6.

Pisutthanan S, Plianbangchang P, Pisutthanan N. Ruanruay S, Muanrit O. Brine shrimp lethality activity of Thai medicinal plants in the family Meliaceae. Nare Univers J. 2004;12:13-8.

Sharififar F, Moshafi MH, Dehghan-Nudehe G, Ameri A, Alishahi F, Pourhemati A. Bioassay screening of the essential oil and various extracts from 4 spices medicinal plants. Pak J Pharm Sci. 2009;22(3):317-22.

Mahmoudvand H, Saedi Dezaki E, Ezatpour B, Sharifi I, Kheirandish F, Rashidipour M. In vitro and in vivo antileishmanial activities of Pistacia vera essential oil. Planta Med. 2016. [Epub ahead of print].

Adams RP. Identification of essential oil components by gas chromatography/mass spectroscopy. Illinois, USA: Carol Stream, IL; 2004: Allured Publishing Corporation.

Mahmoudvand H, Sharififar F, Rahmat MS, Tavakoli R, Dezaki ES, Jahanbakhsh S, Sharifi I. Evaluation of antileishmanial activity and cytotoxicity of the extracts of Berberis vulgaris and Nigella sativa against Leishmania tropica. J Vector Borne Dis. 2014;51(4):294-9.

Trease GE and Evans WC. Pharmacognosy. Bailliere Tindall Press, London, pp.1983;309-706.

Treece GD. Artemia production for marine larval fish culture. SRAC publication. 2000;702:1-7.

Meyer BN, Ferrigni NR Putnam JE. Brine shrimp: A convenient general bioassay for active plant constituents. Planta Medica. 1982;45:31-4.

Elvin-Lewis M. Should we be concerned about herbal remedies? J Ethnopharmacol. 2000;75:141-64.

Salem ML, Hossain MS. Protective effect of black seed oil from Nigella sativa against murine cytomegalovirus infection. Inter J Immunopharmacol. 2000;22:729-40.

Khanna T, Zaidi FA, Dandiya PC. CNS and analgesic studies on Nigella sativa. Fitoterapia. 1993;5:407-10.

Badary OA, Al-Shabanah OA, Nagi MN, Al-Bekairi AM, Almazar MMA. Acute and subchronic toxicity of thymoquinone in mice. Drug Dev Res. 1998;44:56-61.

El Daly ES. Protective effect of cysteine and vitamin E, Crocus sativus and Nigella sativa extracts on cisplatininduced toxicity in rats. J Pharm Belg. 1998;53:87-95.

Prior MG. E valuation brine shrimp (Artemia salina) larvae as a bioassay for mycotoxins in animal feed stuffs. Can J Comp Med. 1979;43:352-5.

Sharififar F, Moshafi MH, Mansouri SH, Khodashenas M, Khoshnoodi M. In vitro evaluation of antibacterial and antioxidant activities of the essential oil and methanol extract of endemic Zataria multiflora Boiss. Food Control. 2004;18:800-5.

23. Saedi Dezaki E, Mahmoudvand H, Sharififar F, Fallahi S, Monzote L, Ezatkhah F. Chemical composition along with anti-leishmanial and cytotoxic activity of Zataria multiflora. Pharm Biol. 2015;8:1-7.




DOI: https://doi.org/10.22087/hmj.v1i2.578

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Lorestan University of Medical Sciences, Khorramabad, Iran.

ISSN: 2538-2144