The Effects of Olive Leaf Extract Administration on the Cerebral Hypoperfusion-Induced Electrophysiological Alterations in Rat Heart

Mehrnoush Moghaddasi, Majid Taati, Hasan Noroozi, Sam Bamani Lirgeshasi, Soheila Pourkhodadad, Bahram Delfan, Afshin Nazari *

Abstract


Background and Aim: In the present study, the role of ethanolic extract of olive (Olea europaea) leaf administration against the cerebral hypoperfusion-induced electrophysiological alterations and left ventricular thickness in rat heart was studied.

Materials and Methods: The right carotid artery was occluded with a 5-0 silk suture and the left carotid artery was occluded after a week. The olive (Olea europaea) leaf extract was administered orally (by gavage) to three experimental groups of rats in 100, 200 and 300 mg/kg/day for two weeks after the left carotid artery occlusion and processed in 25 days. The sham and hypoperfusion groups were fed with normal saline. Each group had 6 rats. After 25 days, electrophysiological heart parameters, i.e. HR, QTc, QRS duration, QT interval, PR interval, RR interval, ST amplitude, T wave amplitude and also the thickness of left ventricle (TLV) were assessed.

Results: The administration of olive leaf extract (100, 200 and 300 mg/kg) exhibited its cardioprotective effect by both stabilizing heart rate and preventing ECG changes (such as shortening of PR, QRS, QT and QTc intervals, prominent T waves and ST elevation). Moreover, olive leaf extract (100, 200 and 300 mg/kg) prevented cardiac hypertrophy after cerebral hypoperfusion.

Conclusion: Our results indicated that post-treatment with ethanolic extract of olive leaves could prove beneficial effects in managing the cerebral hypoperfusion-induced cardiac abnormality in rats.


Keywords


Electrocardiogram, Cerebral hypoperfusion, Left ventricle, Olive leaf extract

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References


van der Bilt IA, Hasan D, Vandertop WP, Wilde AA, Algra A, Visser FC, et al. Impact of cardiac complications on outcome after aneurysmal subarachnoid hemorrhage: a meta-analysis. Neurology. 2009;72(7):635-42.

Zygun D. Non-neurological organ dysfunction in neurocritical care: impact on outcome and etiological considerations. Curr Opin Crit Care. 2005;11(2):139-43.

Gregory T, Smith M. Cardiovascular complications of brain injury. Continuing Education in Anaesthesia, Critical Care & Pain. 2012;12(2).

Barnett HJ. Carotid disease and cognitive dysfunction. Ann Intern Med. 2004;140(4):303-4.

Nguyen H, Zaroff JG. Neurogenic stunned myocardium. Curr Neurol Neurosci Rep. 2009;9(6):486-91.

Tung P, Kopelnik A, Banki N, Ong K, Ko N, Lawton MT, et al. Predictors of neurocardiogenic injury after subarachnoid hemorrhage. Stroke. 2004;35(2):548-51.

El Sedef N, Karakaya S. Olive tree (Olea europaea) leaves: potential beneficial effects on human health. Nutrition reviews. 2009;67(11):632-8.

Andreadou I, Iliodromitis EK, Mikros E, Constantinou M, Agalias A, Magiatis P, et al. The olive constituent oleuropein exhibits anti-ischemic, antioxidative, and hypolipidemic effects in anesthetized rabbits. The journal of nutrition. 2006;136(8):2213-9.

Singh I, Mok M, Christensen A-M, Turner AH, Hawley JA. The effects of polyphenols in olive leaves on platelet function. Nutrition, metabolism and cardiovascular diseases. 2008;18(2):127-32.

Wang L, Geng C, Jiang L, Gong D, Liu D, Yoshimura H, et al. The anti-atherosclerotic effect of olive leaf extract is related to suppressed inflammatory response in rabbits with experimental atherosclerosis. European journal of nutrition. 2008;47(5):235-43.

Mohagheghi F, Bigdeli MR, Rasoulian B, Hashemi P, Pour MR. The neuroprotective effect of olive leaf extract is related to improved blood-brain barrier permeability and brain edema in rat with experimental focal cerebral ischemia. Phytomedicine. 2011;18(2-3):170-5.

Rafighdoost Houshang, Tavafi Majid, Jafari pour Leila, Rasoulian Bahram , Ahmadvand Hassan , Mahmodi Mona , et al. Effect of Olive Leaf Extract inInhibition of Renal Ischemia- Reperfusion Injuries in Rat. ASJ. 2013;10 (3):160-5.

M A, O D, A K, Sh N, A K, A P. Hepatoprotective effects of purified oleuropein from olive leaf extract against ethanol-induced damages in the rat. The Iranian Journal of Veterinary Research. 2012;13(3):218-26.

Leila J, Bahram R, Majid T, Houshang R, Mona M, Marzyeh R, et al. Pretreatment with Olive Leaf Extract Improves Renal and Liver Antioxidant Systems Following Renal Ischemia-Reperfusion Injury in Rats. Herbal Medicines Journal 2016;1(1):37-46

Rezvan V, Nariman M, SeyedJavadSeyed T, Farnaz K, Javad TM, Zahra A-M, et al. Effect of Olive Leaf Extract on Cytokines Secreted by Macrophage. Novelty in Biomedicine. 2016;4(3):116-20(5).

Ayat K, Maryam R, Marzieh r. Oleuropein Reduces Cisplatin-Induced Nephrotoxicity in Human Embryonic Renal Epithelial “GP-293” Cells. Herbal Medicines Journal. 2016;1:18-23.

Esmailidehaj M, Mirhosseini SJ, Rezvani ME, Rasoulian B, Mosaddeghmehrjardi MH, Haghshenas D. Prolonged Oral Administration of Oleuropein Might Protect Heart against Aconitine-induced Arrhythmia. Iran J Pharm Res. 2012;11(4):1255-63.

Mansour E, Shirin B, Ebrahim RM, Mozhdeh S, Zahra H, Zeynab H. Effect of oleuropein on myocardial dysfunction and oxidative stress induced by ischemic-reperfusion injury in isolated rat heart. Journal of Ayurveda and Integrative Medicine. 2016;7(4):224-30.

Marzieh R, Heydari R, Feizbakhsh A, Hashemi P. Rapid Screening of Oleuropein from Olive Leaves Using Matrix Solid-Phase Dispersion and High-Performance Liquid Chromatography. Journal of AOAC International 2014;97(4):1109-13.

Mann DL, Kent RL, Parsons B, Cooper Gt. Adrenergic effects on the biology of the adult mammalian cardiocyte. Circulation. 1992;85(2):790-804.

Ruthirago D, Julayanont P, Tantrachoti P, Kim J, Nugent K. Cardiac Arrhythmias and Abnormal Electrocardiograms After Acute Stroke. Am J Med Sci. 2016;351(1):112-8.

Selvetella G, Notte A, Maffei A, Calistri V, Scamardella V, Frati G, et al. Left ventricular hypertrophy is associated with asymptomatic cerebral damage in hypertensive patients. Stroke. 2003;34(7):1766-70.

Nekooeian AA , Dehghani GA, Mostafavi H, Khalili A. The Effect of Hydroalcoholic Extract of Olive Leaves on Blood Pressure in Rat Model of Two-Kidney, One-Clip Goldblatt Hypertension. Iran Cardiovasc Res J. 2011;5(1):1-6.

Somova LI, Shode FO, Mipando M. Cardiotonic and antidysrhythmic effects of oleanolic and ursolic acids, methyl maslinate and uvaol. Phytomedicine. 2004;11(2-3):121-9.

Molojavyi A, Preckel B, Comfere T, Mullenheim J, Thamer V, Schlack W. Effects of ketamine and its isomers on ischemic preconditioning in the isolated rat heart. Anesthesiology. 2001;94(4):623-9; discussion 5A-6A.

Waterman E, Lockwood B. Active components and clinical applications of olive oil. Altern Med Rev. 2007;12(4):331-42.

Esmailidehaj M, Rasulian B, Rezvani ME, Delfan B, Mosaddeghmehrjardi MH, Pourkhalili K. The anti-infarct, antistunning and antiarrhythmic effects of oleuropein in isolated rat heart. EXCLI J. 2012;11:150-62.




DOI: https://doi.org/10.22087/hmj.v0i0.624

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Lorestan University of Medical Sciences, Khorramabad, Iran.

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