A Stereological Study Concerning the Role of Satureja Khuzestanica Essential Oil in the Improvement of Mesangial Expansion in Uninephrectomized Diabetic Rats

Ahmad Tamjidipour, Majid Tavafi *, Hassan Ahmadvand

Abstract


Background and Aim: Mesangial expansion may lead to renal failure due to the stenosis or occlusion of glomerular capillary lumen in diabetic patients. Oxidative stress is the main cause of diabetic mesangial expansion. The information obtained from the accurate estimation of mesangium volume, glomerular capillary volume and mesangial cells number via stereological methods is more reliable than that of morphometric or semi-quantitative variables in diabetic nephropathy investigations.

Materials and Methods: Forty mature male rats were uninephrectomized and randomly divided into four groups as follows: group 1: the control group, group 2: diabetic without treatment, groups 3 and 4: diabetic treatment with SKEO 250 and 500 ppm in drinking water respectively. The treatment started after the induction of diabetes by alloxan. Treatment blood was sampled and the kidneys were fixed in formal saline solution after 8 weeks. Serum MDA was measured by thiobarbituric acid test. Kidney paraffin sections were prepared and stained via periodic acid Schiff (PAS) method. Mesangium volume, glomerular capillary volume and mesangial cells numbers were estimated via stereological methods. Data were analyzed via Mann Whitney U test at p<0.05.

Results: Diabetes increased serum MDA, mesangium volume, mesangial cells number, and at once decreased glomerular capillary volume in comparison to control group. Treatment by SKEO ameliorated these variables when compared with group 2 (p<0.05).

Conclusion: As an antioxidant agent, SKEO ameliorates diabetic mesangial expansion, glomerular capillary volume and mesangial cells numbers via inhibiting lipid peroxidation.


Keywords


Satureja Khuzestanica, diabetic nephropathy, mesangial expansion, stereology, glomerulosclerosis

Full Text:

PDF

References


Kriz W, Löwen J, Federico G, van den J, Gröne E, Gröne HJ. Accumulation of worn-out GBM material substantially contributes to mesangial matrix expansion in diabetic nephropathy. Am J Physiol Renal Physiol. 2017;312(6):1101-11.

Reidy K, Kang H.M, Hostetter T, Susztak K. Molecular mechanisms of diabetic kidney disease. J Clin Invest. 2014;124:2333-40.

Ostergaard M.V, Pinto V, Stevenson K, Worm J, Fink L.N, Coward R.J. DBA2J db/db mice are susceptible to early albuminuria and glomerulosclerosis that correlate with systemic insulin resistance. Am J Physiol Ren Physiol. 2017;312:312-21.

Satirapoj B. Review on Pathophysiology and Treatment of Diabetic Kidney Disease. J Med Assoc Thai. 2010;93:S228-41.

-Kinaan M, Yau H, Martinez SQ, Kar P. Concepts in diabetic nephropathy: From pathology to treatment. J Ren Hepat Disord. 2017;1(2):10-24.

Abboud HE. Mesangial cell biology. Exp Cell Res. 2012;318:979-85.

Tavafi M. Diabetic nephropathy and antioxi¬dants. J Nephropathology. 2013;2:20-7.

Zhanga L, Panga S, Denga B, Qiana L, Chena J, Zoub J, et al. High glucose induces renal mesan¬gial cell proliferation and fibronectin expression through JNK/NF-κB/NADPH oxidase/ROS path¬way, which is inhibited by resveratrol. Int J Bio¬chem Cell Biol. 2012;44:629–38.

Tavafi M, Ahmadvand H, Tamjidipoor A, Delfan B, Khalatbari AR. Satureja khozestanica essential oil ameliorates progression of diabetic nephropathy in uninephrectomized diabetic rats.

Tissue Cell. 2011;43(1):45-51.

Fernandes N.P, Lagishetty C.V, Panda V.S, Naik S.R. An experimental evaluation of the antidiabetic and antilipidemic properties of a standardized Momordica charantia fruit extract. BMC Complement. Altern Med. 2007;24(7):29-36.

Haidara MA, Mikhailidis DP, Rateb MA, Ahmed ZA, Yassin HZ, et al. Evaluation of the effect of oxidative stress and vitamin E supplementation on renal function in rat with streptozotocin-induced type 1 diabetes. J Diabet Complications. 2009;23(2):130-6.

Drummond K, Mauer M. The Early Natural History of Nephropathy in Type 1 Diabetes. Diabetes. 2002;51(5):1580-7

Gundersen HJG, Bendtsen TF, Korbo L, Marcussen N, Miller A, Nielsen K. Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. APMIS. 1988; 96:379-94.

Fakhruddin S, Alanazi W, Jackson KE.Diabetes-Induced Reactive Oxygen Species: Mechanism of Their Generation and Role in Renal Injury.J Diabetes Res. 2017;2017:8379327.

Schl¨ondorff D, Banas B. The mesangial cell revisited: no cell is an island. JASN. 2009;20:1179-87.

Johansen JS, Harris AK, Rychly DJ, Ergul A . Oxidative stress and the use of antioxidants in diabetes: linking basic science to clinical practice. Cardiovascular Diabetology. 2005;4:5-12.

Satoh M, Fujimoto S, Haruna Y. NAD(P)H oxidase and uncoupled nitric oxide synthase are major sources of glomerular superoxide in rats with experimental diabetic nephropathy. American Journal of Physiology: Renal Physiology. 2005;288:1144-52.

Cheng H, Wang H, Fan X, Paueksakon P, Harris RC. Improvement of endothelial nitric oxide synthase activity retards the progression of diabetic nephropathy in db/db mice. Kidney International. 2012;82:1176-83.

Nakagawa T, Tanabe K, Croker BP, Johnson RJ, Grant MB. Endothelial dysfunction as a potential contributor in diabetic nephropathy. Nature Reviews Nephrology. 2011;7:36–44.

Liapis H, Romagnani P, Anders HJ. New insights into the pathology of podocyte loss: mitotic catastrophe. American Journal of Pathology. 2013;183:1364-74.

Simonson MS. Phenotypic transitions and fibrosis in diabetic nephropathy, Kidney Int. 2007;71:846-54.

Daroux M, Prévost G, Maillard-Lefebvre H, Gaxatte C, D'Agati VD, et al. Advanced glycation end-products: implications for diabetic and non-diabetic nephropathies. Diabetes Metab. 2010;36:1–10.

Marrero MB, Banes-Berceli AK, Stern DM, Eaton DC. Role of the JAK/STAT signaling pathways in diabetic nephropathy, Am J Physiol. 2006;290:762–8.

Abe H, Matsubara T, Arai H. Role of Smad1 in diabetic nephropathy: molecular mechanisms and implications as a diagnostic marker. Histol Histopathol. 2011;26:531-41.

Haase V.H. The sweet side of HIF. Kidney Int. 2010;78:10-13.

Kishi S, Abe H, Akiyama H, Tominaga T, Murakami T, et al. SOX9 protein induces a chondrogenic phenotype of mesangial cells and contributes to advanced diabetic nephropathy. J Biol Chem. 2011;286:32162–69.

Wiggins JE, Patel SR, Shedden KA, Goyal M, Wharram BL, Martini S, Kretzler M, Wiggins RC. J Am Soc Nephrol. 2010;21:587–97.

Tominaga T, Abe H, Ueda O, Goto C, Nakahara K, et al. Activation of bone morphogenetic protein 4 signaling leads to glomerulosclerosis that mimics diabetic nephropathy. J Biol Chem. 2011;286:20109-16.

Winarska K, Malinska D, Szymanski K, Dudziak M, Bryla J. Lipoic acid ameliorates oxidative stress and renal injury in alloxan diabetic rabbits. Biochemie. 2008;90:450-9.

Fujita A, Sasaki H, Doi A, Okamoto K, Matsuno S, Furuta H, Nishi M, Nakao T, et al. Ferulic acid prevents pathological and functional abnormalities of the kidney in otsuka long-Evans Tokushima fatty diabetic rats . Diabet Res Clin Pract. 2008;79:11-17.

C hen X, Wu R, Kong Y, Yang Y, Gao Y, Sun D. Tanshinone IIA attenuates renal damage in STZ-induced diabetic rats via inhibiting oxidative stress and inflammation. Oncotarget. 2017;8(19):31915-22.

Yang Y, Chen G, Cheng X, Teng Z, Cai X, et al. Therapeutic potential of digitoflavone on diabetic nephropathy: nuclear factor erythroid 2-related factor 2-dependent anti-oxidant and anti-inflammatory effect. Sci Rep. 2015;5:12377.

Howard, Vyvyan.; Reed, M. G. Unbiased Stereology : Three-dimensional Measurement in Microscopy Advanced Methods; 2nd. Taylor & Francis Routledge: 2005.

Gundersen HJ, Bendtsen TF, Korbo L, Marcussen N, Moller A, Nielsen K, et al. Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. APMIS. 1988;96(5):379-94.




DOI: https://doi.org/10.22087/hmj.v0i0.673

Refbacks

  • There are currently no refbacks.


This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge.

                               

This work is licensed under a Creative Commons license (CC-BY).  However, the license permits any user to read, copy, redistribute and and make derivative the material in any medium or format for any purpose, even commercially.


 

Lorestan University of Medical Sciences, Khorramabad, Iran.

ISSN: 2538-2144