Subchronic Toxicity of the Ethanolic Extract of Lecaniodiscus cupanioides on Albino Wistar Mice (Mus musculus)
Abstract
Background and Aim: Lecaniodiscus cupanioides has been used in traditional therapies in Nigeria for the management of several ailments. This study investigated its subchronic toxicity. Materials and Methods: 30 mice (20–25g) were placed in three groups of 10 mice. Groups A and B were orally administered 100 and 400 mg/kg of ethanolic extract respectively for 49 days, while group C received distilled water as the control. At expiration of the treatment, the animals were sacrificed to harvest blood and internal organs for hematologic, biochemical, antioxidant and histologic analyses. Results: Hematologic analysis revealed increased monocytes in all the groups. Biochemical and Electrolytes analytes did not show any significant difference. Antioxidant results showed a dose-dependent significant decrease in super oxide dismutase (SOD). Glutathione (GSH) revealed a significant increase in 400mg/kg. No pathology was observed in the heart, but mild toxicity in the kidneys, loss of alveoli architecture in the lungs, inflammation, and congestion of portal triads and central vein in the liver were observed. Increased monocytes observed may depict antibody and anti-infection potentials of the extract. Decreased value of RBC, Hb at higher doses, and mean corpuscular hemoglobin (MCH) at lower doses suggest hemolytic anaemia. Insignificant difference in liver enzymes may suggest extract is not hepatotoxic. Insignificant increased creatinine and urea implies that the extract is not nephrotoxic. Conclusion: The extract displayed a great potential to inhibit free radicals. It increased the antioxidant defense system and GSH at 400mg/kg. Increased SOD and catalase suggest the upregulation of protein which culminates in the clean-up of free radicals and decreasing oxidative stress, thus protecting tissues from extremely reactive radicals.References
Dornelas de Oliveira T, Rodrigues J, Miguel T, Cruz dos Anjos, N, Clemente de Abreu J, et al. Evaluation of Cytotoxicity of Gossypium barbadense L. Extract in Bone Cord Cells through the Micronucleus Test. Journal of Biosciences and Medicines. 2017;5:84-91.
Abbasi A, Khana M, Mushtaq A, Muhammad Z, Sarwat J, Shahzia S. Ethnopharmacological application of medicinal plants to cure skin diseases and in folk cosmetics among the tribal communities of North-West Frontier Province. Ethnopharmacology. 2010;128:322–35.
World Health Organisation (WHO). Traditional Medicine Strategy. Geneva; 2014;10(6):15-20.
Burkill H.M. Brief descriptions and details of the buses of over 4,000 plants. The useful plants of West Tropical Africa. Royal botanical gardens. 2004;3.
Saliu J.A. Redox Potential and Antidiabetic Properties of Lecaniodiscus cupanioides in Vitro. SciFed Journal of Analytical Biochemistry. 2018;1:3.
Nafiu M, Abdulsalam T, Jimoh R, Kazeem M. Ameliorative Effect of Lecaniodiscus cupanioides (Sapindaceae) Aqueous Root Extract in Loperamide-Induced Constipated Rats Tropical Journal of Pharmaceutical Research. 2015;14(6):1057-62.
Ajiboye T, Nurudeen Q, Yakubu M. Aphrodisiac effect of aqueous root extract of Lecaniodiscus cupanioides in sexually impaired rats. Journal of Basic and Clinical Physiology and Pharmacology. 2014;25(2):241-8.
Okore V, Ugwu C, Oleghe, Akpa P. Selective anti-candidal action of crude aqueous pod extract of Lecaniodiscus cupanioides: a preliminary study on Candida albicans obtained from an AIDS patient. Scientific Research and Essays. 2007;2:243-6.
Olugbemiro N, Adedeji A, Adewumi. A. Phytochemical Analysis and Antimalarial Activity Aqueous Extract of Lecaniodiscus cupanioides Root. Journal of Tropical Medicine. 2013; 605393.
Adeyemi O, Yemitan O, Adeogun, O. Analgesic activity of the aqueous root extract of Lecaniodiscus cupanioides. West African Journal of Pharmacological and Drug Research. 2004;20(1-2):10-4.
Yemitan O.K, Adeyemi, O. O. CNS depressant activity of Lecaniodiscus cupanioides, Fitoterapia. 2005;76(5):412–8.
Nne E, Muelsson L, Ndberg G. Structural studies on some saponins from lecaniodiscus cupanioides. Phytochemistry. 1981;8:1939-42.
Adesegun S., Coker H, Hamann, M. Antifungal triterpenoid saponins from Lecaniodiscus cupanioides. Research Journal of Phytochemistry. 2008;2: 93-9.
Costa T, Vieira S, Andrade S, Maistro E. Absence of mutagenicity effects of Psidium cattleyanum Sabine (Myrtaceae) extract on peripheral blood and bone marrow cells of mice. Genetics and Molecular Research. 2008;7:679-86.
Joshua Z, Timothy A. Toxicity Studies of the Aqueous Root Extract of Lecaniodiscus cupanioides on Albino Mice. Science World Journal. 2011;6(4):27-9.
Nagababu E, Rifkind J.M, Sesikeran B, Lakshmaiah N. Assessment of antioxidant activities of eugenol by in vitro and in vivo methods, Methods in Molecular Biology. 2010;610:165–80.
Aebi H. Catalase in vitro. Methods in Enzymology. 1984; 105:121-6.
Thanh T.B, Thanh H.N, Minh H.P.T, Le-Thi-Thu H, Ly H. D T, Duc L.V. Protective effect of Tetracera scandens L. leaf extract against CCl4-induced acute liver injury in rats. Asian Pacific Journal of Tropical Biomedicine. 2015;5:21-7.
Chin Y. W, Balunas M. J, Chai H. B, Kinghorn, A. D. Drug discovery from natural sources. American Association of Pharmaceutical Journal. 2006;14:8(2):239-53.
Pcerangeli G, Vital G, Rivera W. Antimicrobial activity and cytotoxicity of Chromolaenaodorata (L. F.) king and rabinson and Uncariaperrottetii (A. Rich) Merr extracts. Journal of Medicinal Plant Research. 2009; 3(7):511-8.
Sermakkani M, Thangapandian V. GC-MS analysis of Cassia itallica leaf methanol extract. Asian Journal of Pharmaceutical and Clinical Research, 2012;5(2):90-4.
Ferreira SA, Guimarães AG, Ferraria FC, Carneiro CM, Nogueira de Paiva NC, Guimarães, DA. Assessment of acute toxicity of the ethanolic extract of Lychnophora pinaster (Brazilian arnica) Revista Brasilia de Farmacognosia. 2014;24:553-60.
Ajayi A, Ayodele E, Ben-Azu, B, Aderibigbe A, Umukoro S. Evaluation of neurotoxicity and hepatotoxicity effects of acute and sub-acute oral administration of unripe ackee (Blighia sapida) fruit extract. Toxicology Reports. 2019;6:656–65.
Murbach TS, Glavits R, Hirka G, Endres JR, Clewell AE, Szakonyine IP. A 28-day oral toxicology study of an aqueous extract of Polypodium leucotomos (Fernblock®), Toxicology Reports. 2017;4:494–501.
Brosche T, Platt D. Decrease of Cholesterol Concentration in Human Erythrocyte Ghosts in Old Age. Experimental Gerontology Journal. 1990;25(1):23-8.
Iranloye B. Effect of Chronic Garlic Feeding on Some Haematological Parameters. African Journal of Biomedical Research. 2002;5:81-2.
Okunlola D, Olorunisomo A, Aderinola A, Agboola A, Omole O. Haematology and Serum Quality of Red Sokoto Goats fed Baobab Fruit Meal as Supplement to Guinea Grass. Proceedings of the 17th Annual Conference of Annual Science Association of Nigeria. 2012;427-33.
Yoo H, Lee H, Kim J, Yoon J, Lee G, Lee Y, Cho C. A Toxicological Study of HangAmDan-B in Mice. Journal of Acupuncture and Meridian Studies. 2011;4(1):54-60.
Celik K, Kacar E. Kauak S, Bulmus O. Nitrofurantoin Inhibits Contraction of Myometrium Isolated from Pregnant and Non-Pregnant Rats. Medicine Science International Medical Journal. 2018;7(2):315.
Wazis C, Anuka J, Timothy S, Zezi A, Mohammed G, Hussaini I. Acute Toxicity and in-vivo Effects of Leaf Extracts of Byrsocarpus Coccineus Shum &Thonn in Pregnant Rat Uterus. Journal of Applied Pharmaceutical Science. 2012; 2:12, 130-136.
Worasuttayangkurn L, Nakareangrit W, Kwangjai J, Sritangos P, Pholphana, N, Watcharasit P, Rangkadilok, N, Thiantanawat A, Satayavivad J. Acute Oral Toxicity Evaluation of Andrographis paniculata – standardized First True Leaf Ethanolic Extract. Toxicology Reports. 2019;6:426-30.
Aremu O, Adebola O, Opeoluwa O, Benedicta N, Constance R. In Vitro and In Vivo Antioxidant Properties of Taraxacum officinale in Nω-Nitro-l-Arginine Methyl Ester (L-NAME)-Induced Hypertensive Rats. Antioxidants. 2019;8(8):309-19.
Zou S, Yafen W, Qun Z, Yuanyuan L, Yonghui Z, Jinwen Z. Protective effect of Kinsenoside on acute alcohol-induced liver injury in mice. Brazilian Journal of Pharmacognosy. 2019; https://doi.org/10.1016/j.bjp.2019.06.006
Kayode O, Kayode A, Nwonuma C. Alcoholic bitters modulates sex hormones and some biochemical parameters of testicular function in male Wistar rats. F1000Research. 2018;7:1838.
Tasaki M, Umemura T, Maeda M, Ishii Y, Okamura T, Inoue T, Kuroiwa Y, Hirose M., Nishikawa A. Safety assessment of ellagic acid, a food additive, in a subchronic toxicity study using F344 rats. Food and Chemical Toxicology. 2008;46:1119–24.
Beserra A.M, Calegari P.I, Souza M.D, Dos Santos, R.A, Lima J.C, Silva, R.M. Balogun, S.O, Martins D.T. Gastroprotective and ulcer-healing mechansms of ellagic acid in experimental rats. Journal of Agricultural and Food Chemistry. 2011;59:6957–65.
Gaillard Y, Carlier J, Berscht M, Mazoyer C, Bevalot F, Guitton J, Fanton L. Fatal intoxication due to ackee (Blighia sapida) in Suriname and French Guyana. GC-MS detection and quantification of hypoglycin-A, Forensic Science Interval. 2011;206:103–7.
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