Effect of Saffron on serum zinc and copper in patients with metabolic syndrome
AbstractIntroduction: The prevalence of metabolic syndrome (MetS) is increasing globally. Previous studies have shown that saffron has anti-oxidative effects. Copper (Cu) and zinc (Zn) are important trace elements involved in antioxidant protection. We aimed to investigate the effects of saffron supplements on serum levels of Cu, Zn and SOD in subjects with MetS.Methods: This randomized double-blind clinical trial comprised 70 subjects with MetS, aged18-75 years, who referred to the Nutrition Clinic in Ghaem Hospital. They were divided into 2 groups: 1- saffron group: taking a capsule of saffron 100 mg/kg/day (50 mg twice a day) (n=35); 2- placebo group: taking a capsule of placebo (twice a day) (n=35), for a period of 12 weeks. Atomic absorption spectrophotometry was used to measure serum zinc and copper levels in all subjects. Serum SOD activity determined using pyrogallol indirect spectrophotometric assay. SPSS version 18 was used for all the statistical analysis.Results: Fifty-six subjects completed the study. There were no significant differences in baseline features between the saffron and placebo groups (p> 0.05). The changes in serum zinc levels at baseline and after 12 weeks of intervention were significant between the saffron and placebo groups (p=0.041), (Table 2). While, there were no significant differences changes in serum copper, ZN/Cu and SOD1 between 2 study groups before and after the intervention.Conclusion:The results of current study showed that saffron supplementation for a period of 12 weeks can increase serum Zn level in patients with MetS.
Eshaghi FS, Ghazizadeh H, Kazami-Nooreini S, Timar A, Esmaeily H, Mehramiz M, et al. Association of a genetic variant in AKT1 gene with features of the metabolic syndrome. Genes & Diseases. 2019;6(3):290-5.
Ghargerechi R, RAZZAGHY AM. Prevalence of metabolic syndrome in obese children and adolescents dole scents. 2010.
Maleki F. Metabolic syndrome prevalence in Iran: a systematic review and meta-analysis. 2014.
Kermani T, Kazemi T, Molki S, Ilkhani K, Sharifzadeh G, Rajabi O. The efficacy of crocin of saffron (Crocus sativus L.) on the components of metabolic syndrome: A randomized controlled clinical trial. Journal of research in pharmacy practice. 2017;6(4):228.
Walter MF, Jacob RF, Jeffers B, Ghadanfar MM, Preston GM, Buch J, et al. Serum levels of thiobarbituric acid reactive substances predict cardiovascular events in patients with stable coronary artery disease: a longitudinal analysis of the PREVENT study. Journal of the American College of Cardiology. 2004;44(10):1996-2002.
DG H. Gongora MC. Oxidative stress and hypertension. Med Clin North Am. 2009;93(5):621-35.
Sankhla M, Sharma TK, Mathur K, Rathor JS, Butolia V, Gadhok AK, et al. Relationship of oxidative stress with obesity and its role in obesity induced metabolic syndrome. Clinical laboratory. 2012;58(5-6):385-92.
Payne D. Biochemical and Physiological Aspects of Human Nutrition. Journal of the Academy of Nutrition and Dietetics. 2001;101(5):598.
Gómez-García A, Martínez Torres G, Ortega-Pierres LE, Rodríguez-Ayala E, Álvarez-Aguilar C. Rosuvastatin and metformin decrease inflammation and oxidative stress in patients with hypertension and dyslipidemia. Revista espanola de cardiologia. 2007;60(12):1242-9.
Tsimikas S, Willeit J, Knoflach M, Mayr M, Egger G, Notdurfter M, et al. Lipoprotein-associated phospholipase A2 activity, ferritin levels, metabolic syndrome, and 10-year cardiovascular and non-cardiovascular mortality: results from the Bruneck study. European heart journal. 2009;30(1):107-15.
WILLETT W. Nutritional epidemiology: issues and challenges. International journal of epidemiology. 1987;16(2):312-7.
DiSilvestro RA. Zinc in relation to diabetes and oxidative disease. The Journal of nutrition. 2000;130(5):1509S-11S.
Miao X, Sun W, Fu Y, Miao L, Cai L. Zinc homeostasis in the metabolic syndrome and diabetes. Frontiers of medicine. 2013;7(1):31-52.
Hashemipour M, Kelishadi R, Shapouri J, Sarrafzadegan N, Amini M, Tavakoli N, et al. Effect of zinc supplementation on insulin resistance and components of the metabolic syndrome in prepubertal obese children. Hormones. 2009;8(4):279-85.
Suarez Ortegón MF, Ordoñez Betancourth JE, Aguilar de Plata C. Dietary zinc intake is inversely associated to metabolic syndrome in male but not in female urban adolescents. American Journal of Human Biology. 2013;25(4):550-4.
Letelier ME, Sánchez-Jofré S, Peredo-Silva L, Cortés-Troncoso J, Aracena-Parks P. Mechanisms underlying iron and copper ions toxicity in biological systems: Pro-oxidant activity and protein-binding effects. Chemico-biological interactions. 2010;188(1):220-7.
Viktorínová A, Tošerová E, Križko M, Ďuračková Z. Altered metabolism of copper, zinc, and magnesium is associated with increased levels of glycated hemoglobin in patients with diabetes mellitus. Metabolism. 2009;58(10):1477-82.
Arnaud J, De Lorgeril M, Akbaraly T, Salen P, Arnout J, Cappuccio F, et al. Gender differences in copper, zinc and selenium status in diabetic-free metabolic syndrome European population–The IMMIDIET study. Nutrition, Metabolism and Cardiovascular Diseases. 2012;22(6):517-24.
Seo J-A, Song S-W, Han K, Lee K-J, Kim H-N. The associations between serum zinc levels and metabolic syndrome in the Korean population: findings from the 2010 Korean National Health and Nutrition Examination Survey. PloS one. 2014;9(8).
Shafiee M, Aghili Moghaddam NS, Nosrati M, Tousi M, Avan A, Ryzhikov M, et al. Saffron against components of metabolic syndrome: Current status and prospective. Journal of agricultural and food chemistry. 2017;65(50):10837-43.
Kermani T, Mousavi SH, Shemshian M, Norouzy A, Mazidi M, Moezzi A, et al. Saffron supplements modulate serum pro-oxidant-antioxidant balance in patients with metabolic syndrome: A randomized, placebo-controlled clinical trial. Avicenna journal of phytomedicine. 2015;5(5):427.
Gong W, Gottlieb S, Collins J, Blescia A, Dietz H, Goldmuntz E, et al. Mutation analysis of TBX1 in non-deleted patients with features of DGS/VCFS or isolated cardiovascular defects. Journal of medical genetics. 2001;38(12):e45-e.
Zilaee M, Soukhtanloo M, Ghayour-Mobarhan M, Shemshian M, Salehi M, Ferns GA. Effect of saffron on serum leptin levels in patients with metabolic syndrome, a double-blind, randomized and placebo-controlled trial study. PROGRESS IN NUTRITION. 2018;20:140-4.
Ahmed H, Schott EJ, Gauthier JD, Vasta GR. Superoxide dismutases from the oyster parasite Perkinsus marinus: purification, biochemical characterization, and development of a plate microassay for activity. Analytical biochemistry. 2003 Jul 1;318(1):132-41.
Safarian H, Parizadeh SMR, Saberi-Karimain M, Darroudi S, Javandoost A, Mohammadi F, et al. The effect of curcumin on serum copper and zinc and Zn/Cu ratio in individuals with metabolic syndrome: a double-blind clinical trial. Journal of dietary supplements. 2019;16(6):625-34.
Abolbashari S, Darroudi S, Tayefi M, et al. Association between serum zinc and copper levels and antioxidant defense in subjects infected with human T-lymphotropic virus type 1. J Blood Med. 2018;10:29–35. Published 2018 Dec 27. doi:10.2147/JBM.S184913
Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European journal of biochemistry. 1974;47(3):469-74.
Hosseini A, Razavi BM, Hosseinzadeh H. Saffron (Crocus sativus) petal as a new pharmacological target: a review. Iranian Journal of Basic Medical Sciences. 2018 Nov;21(11):1091-1099. DOI: 10.22038/IJBMS.2018.31243.7529.
Modaghegh M-H, Shahabian M, Esmaeili H-A, Rajbai O, Hosseinzadeh H. Safety evaluation of saffron (Crocus sativus) tablets in healthy volunteers. Phytomedicine. 2008;15(12):1032-7.
Hajian Tilaki K, Heidari B. Prevalence of obesity, central obesity and the associated factors in urban population aged 20–70 years, in the north of Iran: a population based study and regression approach. Obesity reviews. 2007;8(1):3-10.
Consortium LF-N. Understanding local Mediterranean diets: a multidisciplinary pharmacological and ethnobotanical approach. Pharmacological Research. 2005;52(4):353-66.
Kermanshahi H, Riasi A. Effect of dietary dried Berberis vulgaris fruit and enzyme on some blood parameters of laying hens fed wheat-soybean based diets. Int J Poult Sci. 2006;5:89-93.
Nikbakht-Jam I, Khademi M, Nosrati M, Eslami S, Foroutan-Tanha M, Sahebkar A, et al. Effect of crocin extracted from saffron on pro-oxidant–anti-oxidant balance in subjects with metabolic syndrome: a randomized, placebo-controlled clinical trial. European Journal of Integrative Medicine. 2016;8(3):307-12.
Makhlouf H, Saksouk M, Habib J, Chahine R. Determination of antioxidant activity of saffron taken from the flower of Crocus sativus grown in Lebanon. African Journal of Biotechnology. 2011;10(41):8093-100.
Rahaiee S, Moini S, Hashemi M, Shojaosadati SA. Evaluation of antioxidant activities of bioactive compounds and various extracts obtained from saffron (Crocus sativus L.): a review. J Food Sci Technol. 2015;52(4):1881–1888. doi:10.1007/s13197-013-1238-x
Goli SAH, Mokhtari F, Rahimmalek M. Phenolic compounds and antioxidant activity from saffron (Crocus sativus L.) petal. Journal of Agricultural Science. 2012;4(10):175.
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