A Comparative Study of the Antibacterial Effect of Three Ethnomedical Plants (Ocimum gratissimum, Vernonia amygdalina and Cymbopogon citratus) on Certain Clinical Isolates

  • Obhioze Augustine Akpoka * Department of Biological Science, College of Natural and Applied Sciences, Igbinedion University, Okada, Nigeria https://orcid.org/0000-0001-9190-9188
  • Maureen U. Okwu Department of Biological Science, College of Natural and Applied Sciences, Igbinedion University, Okada, Nigeria
  • Odaro Stanley Imade Department of Biological Science, College of Natural and Applied Sciences, Igbinedion University, Okada, Nigeria
  • Spencer C. O. Nwangwu Department of Biochemistry, College of Natural and Applied Sciences, Igbinedion University, Okada, Nigeria
  • Erifeta Georgina Omonegho Department of Biochemistry, College of Natural and Applied Sciences, Igbinedion University, Okada, Nigeria
  • Christabel Uti Department of Biological Science, College of Natural and Applied Sciences, Igbinedion University, Okada, Nigeria
Keywords: Ethnomedical plants, Therapeutic efficacy, Ocimum gratissimum, Vernonia amygdalina and Cymbopogon citratus

Abstract

Background and Aim: Ethnomedicinal plants are used by indigenous populations all over the world as remedies for various maladies. The present study aimed at evaluating the antibacterial susceptibility of the leaves of Cymbopogon citrates, Vernonia amygdalina and Ocimum gratissimum against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and methicillin-resistant Staphylococcus aureus.Materials and Methods: Active agents in the leaves were extracted with methanol using the Soxhlet extraction technique. The extracts were then tested for antibacterial activity using the agar well diffusion method. MIC was determined by the tube dilution technique.Results: The results revealed that the methanolic extract of C. citrates had the best mean zones of inhibition against P. aeruginosa, S. aureus, E. coli and methicillin-resistant S. aureus. The mean zones of the inhibition of C. citrates against P. aeruginosa, S. aureus, E. coli and methicillin-resistant S. aureus were 11.5 ± 1.5 mm (31.25 mg/ml), 11.5 ± 0.5 mm (125 mg/ml), 12.0 ± 1.0 (125 mg/ml) and 12.0 ± 1.0 (500 mg/ml) respectively. The activities of the extracts in relation to the activity of gentamycin (positive control) and DMSO (negative control) were also determined. The methanolic extract of C. citrates had the highest activity (38 %) against Pseudomonas aeruginosa at 31.25 mg/ml and S. aureus (54%) at 250 mg/ml and (52%) against MRSA at 500 mg/ml. V. amygdalin showed the highest activity (35%) against E. coli at 125 mg/ml. The therapeutic efficacy was also determined using the breakpoint of 10 µg gentamycin (the positive control).Conclusion: The findings of this study suggest that C. citrates, V. amygdalina and O. gratissimum could be explored by pharmaceutical companies as raw materials for the synthesis of new antibiotics for the treatment of bacterial infections, particularly as a cocktail.

References

Ossy MJK, Jean-Marie TC, Ngenda MPS, Lusamba D. African Health Monitor. A magazine of the World Health Organization Regional Office for Africa. 2003;4(1).

Principe P. Monetizing the pharmacological benefits of plants. Washington D.C. : US Environmental protection Agency , 1991.

Diallo D, Hveem B, Mahmoud MA, Berge G, Paulsen BS, Maiga A. An ethnobotanical survey of herbal drugs of Gourma district Mali. Pharm. Biol. 1999;37:80-91.

Nascimento GGF, Locatelli J, Freitas PC, Silva GL Antibacterial activity of plant extracts and phytochemical on antibacterial-resistant bacteria. Braz. J. Microbiol. 2000;314:247-56.

Vierra RF, Simon JE. Chemical characterization of Ocimum gratissimum found in the market and used in Traditional medicine in Brazil. Journal of Economic Botany. 2000;20:5-6.

Iwu MM. Handbook of African medicinal plants. s.l. : CRC Press Inc. Boca Raton Floridamm, 1993.

Owulade MO, Eghianruwa KI, Daramola FO. Effects of aqueous extracts of Hibiscus sabdariffa calyces and Ocimum gratissimum leaves on intestinal transit in rats. Afr. J. Biomed Res. 2004;7:31-33.

Akinmoladun AC, Ibukun EO, Afor E, Obuotor EM, Farombi EO. Phytochemical constituent and antioxidant activity of extract from the leaves of Ocimum gratissimum. Sci Res Essay. 2007;2:63-166.

Matasyoh LG, Josphat CM, Francis NW, Miriam GK, Anne WTM, Titus KM. Chemical Composition and Antimicrobial Activity of the Essential Oil of Ocimum gratissimum L Growing in Eastern Kenya. African Journal of Biotechnology. 2007;6:760-5.

Kabir OA, Olukayode O, Chidi EO, Christopher CI, Kehinde AF. Screening of crude extracts of six medicinal plants used in South-West Nigerian unorthodox medicine for anti-methicillin resistant Staphylococcus aureus activity. BMC Complement Altern Med. 2005;5:1-7.

Effraim KD, Jacks TW, Sodipo OA. Histopathological studies on the toxicity ofOcimum gratissimum leaves extract on some on some organs of rabbit. Afr J Biomed Res. 2003;6:21-5.

Adebolu TT, Salau AO. Antimicrobial activity of leaf extracts of Ocimum gratissimum on selected diarrhea causing bacteria in south western Nigeria. Afr J Biotech. 2005;4:682-4.

Ngassoum MB, Jessia-Ngang JJ. Tatsadjieu LN, Jirovetz L, Buchbauer G, Adjoudji O. Antimicrobial study of essential oils of Ocimum gratissimum leaves and Zanthoxylum xanthoxyloids fruits from Cameroom. Filtoterapia. 2003;74:284-7.

Orafidiya LO, Fakoya FA, Agbani EO, Iwalewa EO. Vascular permeability increasing effect of the leaf essential oil of Ocimum gratissimum Linn as a mechanism for its wound healing property. Afr J Trad. 2005;2:253-58.

Igile GO, Oleszek W, Jurzysta M, Burda S, Fafunso M, Fasanmade AA.Flavonoids from Veronia amugdalina and their antioxidant activities. J Agric Food Chem. 1994;42;2445-8.

Butter GW, Bailey RW,. Chemistry and biochemistry of Herbage. Academic Press London; 1973;1.

Lee HJ, Jeong HS, Kim DJ, Noh YH, Yuk DY, Hong JT. Inhibitory effect of citral on NO production by suppression of iNOS expression and NF-kappa B activation in RAW2647 cells. Archives of Pharmacal Research. 2008;31:342-9.

Tiwari M, Dwivedi UN, Kakkar P. Suppression of oxidative stress and pro-inflammatory mediators by Cymbopogon citratus D Staph extract in lipopolysaccharide stimulated murine alveolar macrophages, Food Chem Toxicol. 2010;48:2913-9.

Sunita B, Mahendra R. Antifungal Activity of Essential Oils from Indian Medicinal Plants Against Human Pathogenic Aspergillus fumigatus and A niger. World Journal of Medical Sciences. 2008;48:81-8.

Abosi AO, Raseroka BH. In vivo antimalarial activity of Vernonia amygdalina. Br J of BioMed Sci. 2003;60(20):89-91.

Izevbigie EB, Bryant JL, Walker A. A novel natural inhibitor of extra cellular signal related kinases and human breast cancer cell growth. Maywood Exp Biol Med. 2004;228:293-8.

Cheesbrough M. Medical laboratory manual for tropical countries. ELBS edition. Tropical Health Technology Publications, UK, 2002.

Murugan S, Anand R, Uma -Devi, P, Vidhya N, Rajesh KA. Efficacy of Euphobia milli and E. pulcherrima on aflatoxin producing fungi (Aspergillus flavus and A. parasiticus). African Journal of Biotechnology. 2007;6(6):718-9.

Perez C, Pauli M, Bazevque P. An antibiotic assay by the agar well diffusion method. Acta Biol et Med Experimentalis. 1990;15:113-5.

Rojas JJ, Veronica JO, Saul AO, Muñoz JF. Screening for antimicrobial activity of ten medicinal plants used in Colombian folkloric medicine: A possible alternative in the treatment of non-nosocomial infections.. 2006, BMC Complemen Altern Med. 2006;6:2.

Figueirinha A, Cruz MT, Francisco V, Lopes MC, Batista MT. Anti-inflammatory activity of Cymbopogon citratus leaf infusion in lipopolysaccharide-stimulated dendritic cells: contribution of the polyphenols. Journal of Medicinal Food. 2010;13(3):681-90.

Baron JE, Fingold SM. Methods for Testing Antimicrobial Effectiveness . In: Bailey Scotts Diagnostic Microbiology. [ed.] Mosby C V. (ed) Missouri. 1990;171-94.

Microbeonline. [Online] https://microbeonlinecom/minimum-inhibitory-concentration-mic-broth-dilution-method-procedure-interpretation accessed, 2018.

CLSI. Performance Standards for Antimicrobial Susceptibility Testing. Twenty-Second Informational Supplement CLSI document M100- S22. Wayne PA : Clinical and Laboratory Standards Institute, 2012.

Singariya P, Mourya KK, Kumar P. Antimicrobial activity of the crude extracts of Withania somnifera and Cenchrus setigerus in-vitro. Pharmacognosy. 2011; J3:60-5.

Sharma B, Kumar P. Extraction and pharmacological evaluation of some extracts of Tridax procumbens and Capparis decidua. J Appl Resh Nat Prod. 2009;1:5-12.

Leite JR, Seabra M, Maluf L. Pharmacology of lemongrass Cymbopogon citratus Stapf III Assessment of eventual toxic hypnotic and anxiolytic effects on humans. J Ethnopharmacol. 1986;17(1):75–83.

Barrow G I, Feltham RKA. Cowan and Steel’s Manual of Medical Bacteria . 3rd ed : Cambridge University Press. 2000; p. 352.

Simplice DK, Llnoudo DP, Wendyame MC, Nadembega YA, Ajeneba O, Silvatore P, Virginio P, Alfred ST, Comlar de S, Jacques S. Antimicrobial resistance of major urinary tract pathogens. Pak J Biolog Sci. 2009;12:712-6.

Jafari B, Ebadi A, Aghdam BM, Hassanzade Z. Antibacterial activities of lemongrass methanol extract and essence on pathogenic bacteria. American-Eurasian J Agric and Environ Sci, 2012;2:1042-6.

Balakrishnan B, Paramasivam S, Arulkumar A. Evaluation of the lemongrass plant Cymbopogon citratus extracted in different solvents for antioxidant and antibacterial activity against human pathogens. Asian Pac J Trop Dis. 2009;4:S134-S139.

Duyilemi OP, Lawal IO. Antibacterial activity and phytochemical screening of Chrysophyllum albidum leaves. Asian Journal of Food and Agro-Industry, 2009; Special Issue: S75-S79.

Ibrahim TA, Ajala L, Adetuyi FO, Ojei JB. Assessment of the antibacterial activity of Vernonia amygdalina and Occimum gratissimum leaves on selected food borne pathogens. Int J Third World Med. 2009;8:1539-45.

Okwu MU, Olley M, Akpoka AO, Izevbuwa OE. Methicillin-resistant Staphylococcus aureus MRSA and anti-MRSA activities of extracts of some medicinal plants: A brief review. AIMS Microbiology. 2019;52:17–137.

Published
2019-10-03
Section
Original Article