Reproductive Toxicity of Ethanolic Extract of Lecaniodiscus cupanioides on Male Albino Mice

  • Adeola Michael Oloyede * Department of Cell Biology and Genetics, University of Lagos, Akoka, Lagos-Nigeria
  • Chidalu Christabel Anyika Department of Cell Biology and Genetics, University of Lagos, Akoka, Lagos-Nigeria
  • Bodunrin Ottu Department of Cell Biology and Genetics, University of Lagos, Akoka, Lagos-Nigeria
Keywords: L. cupanioides, Sperm, Abnormality, Testes, Toxicity


Background and Aim: Lecaniodiscus cupanioides is one of the numerous plants therapeutically used in  Southwestern Nigeria for controlling and curing several health complications. In this study, the in vivo effects of the ethanolic extract of L. cupanioides on sperm morphology of albino mice were investigated.Materials and Methods: Fifteen mice (18.2g to 24.8g) were divided into 3 groups of 5 mice. The treatment groups were orally administered 100 and 400mg/kg of the extract, and the control group received distilled water for 49 days. At the expiration of exposure, the mice were sacrificed by jugular puncture. Epididymis and testes were harvested for sperm cytological analysis and testes histology.Results: Fourteen types of sperm morphological abnormalities observed included: no hook, head with double tails, knobbed hook, amorphous head, bent hook, hook at wrong angle, tail folded over head, pin head, banana-shaped head, tail with no head (prominent), tail with double heads, head with triple tails, wrong tail attachment and head with no tail. Incidence was not significantly different from the control. Furthermore, no correlation between the dose level of the administered drug and incidence of abnormal sperms was observed. Hence, various abnormalities might probably be adduced to natural mistakes during differentiation in spermiogenesis, chromosomal aberration during packaging of genetic material in sperm head, occurrence of small deletions and point mutation during spermatogenesis. Histology of the testes did not show any malformation or alteration, which implies a highly conserved histoachitecture.Conclusion: This suggests that the extract might be devoid of any toxic effect on the male reproductive organs and sperms at the doses evaluated within the exposure period

Author Biography

Adeola Michael Oloyede *, Department of Cell Biology and Genetics, University of Lagos, Akoka, Lagos-Nigeria
Senior LecturerDepartment of Cell Biology and Genetics,Faculty of Science,University of Lagos,Akoka, Yaba- Lagos


Abe AI, Wakeel OK, Olapade M K. Bioassay-guided fractionation of antinociceptive effect of Lecaniodiscus cupanioides using mice. European Journal of Pharmaceutical and Medical Research. 2019;6(2):88-95.

Nafiu M, Abdulsalam T, Jimoh, R, Kazeem M. Ameliorative Effect of Lecaniodiscus cupanioides (Sapindaceae) Aqueous Root Extract in Loperamide-Induced Constipated Rats Tropical Journal of Pharmaceutical Research. 2015;14(6):1057-62.

Olaide NO, Ajiboye TO. Aqueous root extract of Lecaniodiscus cupanioides restores the alterations in testicular parameters of sexually impaired male rats Asian Pacific Journal of Reproduction. 2012;1(2):120-4.

Yemitan OK, Adeyemi OO. CNS depressant activity of Lecaniodiscus cupanioides, Fitoterapia. 2005;76(5):412-8.

Adeyemi OO, Yemitan OK, Adeogun OO. Analgesic activity of the aqueous root extract of Lecaniodiscus cupanioides. West African Journal of Pharmacological and Drug Research. 2004;20 (1-2):10-4.

Oloyede AM. The Reproductive Toxicity of the Ethanolic Extract of Tetrapleura tetraptera Pods on Male and Female Swiss Albino Mice (Mus musculus). Herbal Medicines Journal. 2018;3(1):1-7.

Otunbajo OA, Mosuro AA, Ladipo TF. An in vivo Evaluation of Induction of Abnormal Sperm Morphology by Invermectin MSD (Mectizan). Pakistan Journal of Biological Sciences. 2007;10(1):90-5.

Bakare AA, Adekunle A, Mosuro AA, Osibanjo O. An in vivo evaluation of induction of abnormal sperm morphology in mice by landfill leachates. Mutation Research. 2005;582:28-34.

Otitoloju AA, Obe IA, Adewale OA, Otubanjo OA, Osunkalu VO. Preliminary study on the induction of sperm head abnormalities in mice, Mus musculus, exposed to radiofrequency radiations from global system for mobile communication base stations. Bulletin of Environmental Contamination Toxicology. 2010;84:51-4.

Agunbiade SO, Okonko IO, Alimba CG, Folarin AC, Anugweje KC. Effects of a carbonaceous bottling plant effluent on albino mice sperm morphology and testes histopathology. Natural Science. 2012;10:154-60.

Rasgele PG. Abnormal sperm morphology in mouse germ cells after short – term exposures to acetamiprid, propineb, and their mixture. Arh Hig Rada Toksikol. 2014;65:47-56.

Narayana K, D’Souza UJ, Seetharama Rao KP. Ribavirin-induced sperm shape abnormalities in Wistar rat. Mutation Research. 2002;513:193-6.

Mendoza-Lujambio I, Burfeind P, Dixkens C, Meinhardt A, Hoyer-Fender S, Engel W, et al. The Hook1 gene is non-functional in the abnormal spermatozoon head shape (azh) mutant mouse. Human Molecular Genetics. 2002;11:1647-58 .

Pyle A, Handel MA. Meiosis in male PL/J mice: a genetic model for gametic aneuploidy. Molecular Reproduction and Development. 2003;64:471-81.

Escalier D, Bai XY, Silvius D, Xu PX, Xu, X. 2003. Spermatid nuclear and sperm periaxonemal anomalies in the Mouse Ube2b null mutant. Molecular Reproduction and Development 65:298-308.

Lamara AS, Fonseca G, Fuentes JL, Cozzi R, Cundari E, Fiore M, et al. Assessment of the genotoxic risk of Punica granatum L. (Punicaceae) whole fruit extracts. Journal of Ethnopharmacology. 2008;115:416-22.

Original Article