Herbal Medicines Journal
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Evaluation of the Antibacterial Effect of Garlic and Ginger on ESBL and KPC-Producing Pseudomonas aeruginosa Strains

Document Type : Original Article

Authors

Nooshin Gharibi 1
Mohsen Mirzaee 2
Pegah Shakib 3

1 Department of Microbiology, Borujerd Branch, Islamic Azad University, Borujerd, Iran

2 Department of Laboratory Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran

3 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

10.22087/herb%20med%20j.v5i2.781
Abstract
Background and Aim: Due to the problems caused by the formation of biofilms in industry and medicine as well as the development of drug resistance, new methods are required to inhibit resistant microorganisms, particularly in the biofilm form. The aim of the present research was to determine the effect of garlic and ginger on the biofilm formation of Pseudomonas aeruginosa. Materials and Methods: In this study, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of garlic and ginger extracts against Pseudomonas aeruginosa were assessed by microtiter plate method. Biofilm formation was investigated by the use of microtiter plate method and staining with crystal violets. Results: The minimum inhibitory concentrations of garlic and ginger extracts against ATCC 27853 standard strain Pseudomonas aeruginosa were 40 and 2.5 mg / ml respectively. Moreover, it was 80 and 20 mg / ml for ESBL, KPC and ESBL + KPC generating strains respectively. The mean percentages of the biofilm inhibition of Pseudomonas aeruginosa by garlic and ginger extracts were 61.98% and 66.81% respectively. Conclusion: Garlic and ginger extract might be used in different compounds to inhibit Pseudomonas aeruginosa and its biofilm formation. 

Keywords

Biofilm
Garlic
Ginger
Pseudomonas aeruginosa
Minimum Inhibitory Concentration (MIC)
Minimum Bactericidal Concentration (MBC)
Herbal Medicines Journal
Volume 5, Issue 2
Spring 2020
Pages 60-68

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