Background and Aim: In the present study, a stainless steel plate was penetrated and platinized, and it was used as the disk for the solid phase dynamic extraction (SPDE) method. The SPE disk was coated by polythiophene/multiwalled carbon nanotubes (PTh/MWCNTs) nanocomposite using in-situ electropolymerization method.Materials and Methods: A high-surface area, porous and unbreakable platinized SPE disk was used for the dynamic extraction and determination of thymol and carvacrol by high-performance liquid chromatography (HPLC) with UV detection. The effect of various variables, including the type of elution solvent, pH, ionic strength and extraction time was optimized by the use of the central composite design (CCD) method.Results: Under the response surface methodology, the present method has admissible calibration curves over wide linear ranges of 0.01-100 µg mL-1 with high coefficient of determination (0.9998) that firmly confirms the high pertinence of the present method to the quantification of analytes. Meanwhile, their limits of detection (LODs) were 0.007 and 0.005 µg mL-1, and the limits of quantification (LOQs) were 0.09 and 0.01 µg mL-1 for thymol and carvacrol, respectively. Moreover, the enrichment factor (EF) was 87 for thymol and 54 for carvacrol, and the preconcentration factor was 142.8 for thymol and carvacrol.Conclusion: The SPDE-PTh/MWCNTs-disk was used for the extraction of thymol and carvacrol in thyme samples, and satisfactory results were obtained