Background and Aim: Some medicinal plants are conventionally used for treatment of different aspects of male infertility. This study was conducted to evaluate the effects of Hibiscus Sabdariffa aqueous extract on spermatogenesis and sperm parameters of mice.materials and methods: Forty-eight were divided into 4 groups with 3 replications and 3 animals in per replication and received doses of 0, 100, 200 and 300 mg/kg of Hibiscus Sabdariffa aqueous extract for 28 days. Levels of testosterone, LH, FSH, TAS, SOD, GPx, blood variables, testis weight, sperm abnormality and viability, motility and sperm concentration were evaluated.Results: Oral administration of Hibiscus Sabdariffa aqueous extract lowered levels of FSH and LH in comparison to control group (P<0.05). The level of TAS was significantly lower in level of 300 mg/kg of the extract in comparison to control group (P<0.05).  The levels of SOD and MDA were significantly higher and lower in comparison to control group, respectively. The levels of triglycerides and cholesterol were significantly lower in control group in comparison to other groups (P<0.05). Left and right weights were significantly lower in 100 and 200 mg/kg extract groups in comparison to 300 mg/kg extract group (P<0.05). Normal sperm count was significantly higher in mice treated with 100 mg/kg of the extract. Defected sperms were significantly lower in mice treated with extract.Conclusion: It cannot be stated to use of clear dose of Hibiscus Sabdariffa aqueous extract in order to improve spermatogenesis and sperm parameters in mice.