1
Department of Physiology and Pharmacology, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
2
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Abstract
Background and Aim:The protective effect of oleuropein,a chief polyphenol compound in olive tree, on cisplatin-induced nephrotoxicity in human cultured renal tubular cells, GP-293, was investigated.Materials and Methods:Cell viability was determined by the MTT assay. Cleaved caspase-3 and Bax: Bcl2 ratioas biochemical parameters of cellular apoptosis, was assessed using western blot analysis.Results:Our data showed that cisplatin in the dose of 55µg/ml significantly can reduced cell viability, increased caspase-3 activation and Bax: Bcl2 ratio in GP-293 cells after 24hr. Incubation of the cisplatin treated cells, with 20μg/ml oleuropein decreased the cisplatin-induced cell toxicity, as well as prevented caspase-3 activation and reduced Bax: Bcl2 elevation ratio.Conclusions: Oleuropein protects against cisplatin-induced cellular toxicity. This could be associated with the prevention of cellular apoptosis
Kaeidi,A , Rezaei,M and rashidipour,M . (2016). Oleuropein Reduces Cisplatin-Induced Nephrotoxicity in Human Embryonic Renal Epithelial “GP-293” Cells. Herbal Medicines Journal, 1(1), 18-23.
MLA
Kaeidi,A , , Rezaei,M , and rashidipour,M . "Oleuropein Reduces Cisplatin-Induced Nephrotoxicity in Human Embryonic Renal Epithelial “GP-293” Cells", Herbal Medicines Journal, 1, 1, 2016, 18-23.
HARVARD
Kaeidi A, Rezaei M, rashidipour M. (2016). 'Oleuropein Reduces Cisplatin-Induced Nephrotoxicity in Human Embryonic Renal Epithelial “GP-293” Cells', Herbal Medicines Journal, 1(1), pp. 18-23.
CHICAGO
A Kaeidi, M Rezaei and M rashidipour, "Oleuropein Reduces Cisplatin-Induced Nephrotoxicity in Human Embryonic Renal Epithelial “GP-293” Cells," Herbal Medicines Journal, 1 1 (2016): 18-23,
VANCOUVER
Kaeidi A, Rezaei M, rashidipour M. Oleuropein Reduces Cisplatin-Induced Nephrotoxicity in Human Embryonic Renal Epithelial “GP-293” Cells. Herb Med J. 2016;1(1):18-23.
