Herbal Medicines Journal
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Oleuropein Reduces Cisplatin-Induced Nephrotoxicity in Human Embryonic Renal Epithelial “GP-293” Cells

Document Type : Original Article

Authors

Ayat Kaeidi 1
Maryam Rezaei 2
Marzieh rashidipour 2

1 Department of Physiology and Pharmacology, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

2 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

Abstract
Background and Aim:The protective effect of oleuropein,a chief polyphenol compound in olive tree, on cisplatin-induced nephrotoxicity in human cultured renal tubular cells, GP-293, was investigated.Materials and Methods:Cell viability was determined by the MTT assay. Cleaved caspase-3 and Bax: Bcl2 ratioas biochemical parameters of cellular apoptosis, was assessed using western blot analysis.Results:Our data showed that cisplatin in the dose of 55µg/ml significantly can reduced cell viability, increased caspase-3 activation and Bax: Bcl2 ratio in GP-293 cells after 24hr. Incubation of the cisplatin treated cells, with 20μg/ml oleuropein decreased the cisplatin-induced cell toxicity, as well as prevented caspase-3 activation and reduced Bax: Bcl2 elevation ratio.Conclusions: Oleuropein protects against cisplatin-induced cellular toxicity. This could be associated with the prevention of cellular apoptosis

Keywords

Cisplatin
Nephrotoxicity
Apoptosis
GP-293 cells
Oleuropein
Herbal Medicines Journal
Volume 1, Issue 1
Spring 2016
Pages 18-23

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