Herbal Medicines Journal
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A Comparative Evaluation of the Antioxidant and Phytochemical Properties of Various Extracts of Ocimum Sanctum Linn Grown in Jaffna District, Sri Lanka

Document Type : Original Article

Authors

Gowri Rajkumar 1
Vilochana Alahendra 1
Vinotha Sanmugarajah 2

1 Department of Botany, Faculty of Science, University of Jaffna, Sri Lanka

2 Department of NoiNaadal Chikitchai, Faculty of Siddha Medicine, University of Jaffna, Sri Lanka

https://doi.org/10.22087/hmj.2025.231632
Abstract
Background and Aim: Ocimum sanctum Linn (O. sanctum) (Lamiaceae) is a valuable medicinal herb. It contains several therapeutic characteristics due to its bioactive components. This study aimed to compare the phytochemical and antioxidant properties of different extracts of the leaves and flowers of the O. sanctum plant, which grows in Sri Lanka.
Materials and Methods: Standard procedures were followed for the qualitative and quantitative phytochemical analyses and the DPPH as well as ABTS antioxidant activities.
Results: The findings demonstrated that the ethanolic and methanolic extracts had adequate concentrations of flavonoids, phenols, tannins, and alkaloids compared with the aqueous extracts of the flowers and leaves of O. sanctum. The supreme quantity of the flavonoid content existed in the ethanolic flower extracts (50.88±0.62 µg QE/g) and methanolic leaf extract (49.11±0.27 µg QE/g), while the higher amount of the phenol content (20.96±0.65 µg GAE/g) was found in the ethanolic leaf extract. The methanolic leaf extract had the highest tannin content (409.95±0.66 µg TAE/g) compared with other extracts.  Moreover, the highest amount of alkaloid was observed in flowers (75.83±0.55 mg/g) and not in leaves (52.60±0.66 mg/g). Furthermore, the methanolic flower extract exhibited the greatest DPPH (IC50: 0.68±0.19μg/mL) and ABTS (IC50: 1.82±0.32μg/mL) activities compared with the Trolox.
Conclusion: The present study proved that although the leaf extracts contained higher concentrations of phytochemicals, the methanolic flower extract of O. sanctum had a greater potential for antioxidants than the methanolic leaf extract.

Keywords

Antioxidant
Comparative evaluation
Various extracts
Ocimum sanctum
Phytochemicals
Sri Lanka
  1. Sofowora A, Ogunbodede E, Onayade A. The role and place of medicinal plants in the strategies for disease prevention. Afr J Tradit Complement altern med. 2013; 10(5): 210-229.
  2. WHO. Research Guidelines for Evaluating the safety and Efficacy of Herbal Medicines, World Health Organization, Manila;1992.
  3. Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol. 2014; 4:177.
  4. Yuan H, Ma Q, Ye L, Piao G. The traditional medicine and modern medicine from natural products. Molecules. 2016; 21(5): 559.
  5. Tungmunnithum D, Thongboonyou A, Pholboon A, Yangsabai A. Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: An overview. Medicines. 2018; 5(3): 93.
  6. Tran N, Pham B, Le L. Bioactive compounds in anti-diabetic plants: From herbal medicine to modern drug discovery. Biology. 2020; 9(9): 252.
  7. Shehadeh MB, Suaifan GA, Abu-Odeh AM. Plants secondary metabolites as blood glucose-lowering molecules. Molecules. 2021; 26(14): 4333.
  8. Niaz A, Adnan A, Bashir R, Mumtaz MW, Raza SA, Rashid U, Tan CP, Tan TB. The In Vitro α-Glucosidase Inhibition Activity of Various Solvent Fractions of Tamarix dioica and 1H-NMR Based Metabolite Identification and Molecular Docking Analysis. Plants. 2021;10(6):1128.
  9. Altemimi A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot DA. Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants. 2017; 6(4): 42.
  10. Mahmood K. Antibacterial activity of essential oil of Ocimum sanctum L. Mycopath. 2012; 6 (1 & 2): 63-65.
  11. Singh JP, Singh AK, Bajpai A, Ahmad IZ. Characterization of different Syzygium cumini skeels accessions based on physico-chemical attributes and phytochemical investigations. Int J Pharm Pharm Sci. 2015; 7(5): 158-164.
  12. Pattanayak P, Behera P, Das D, Panda SK. Ocimum sanctum Linn. A reservoir plant for therapeutic applications: An overview. Pharm Rev. 2010; 4(7): 95-105. doi: 10.4103/0973-7847.65323. PMID: 22228948; PMCID: PMC3249909.
  13. Sen P. Therapeutic potential of Tulsi: From experience to facts. Drug News Views. 1993; 1: 15-21.
  14. Sajjan. Therapeutic Uses of “Wonder Herb” Tulsi (Ocimum sanctum Linn) - A Review. Research Biotica. 2021; 3(1): 57-62.
  15. Datiles MJ, Acevedo-Rodríguez P. Ocimum tenuiflorum (holy basil). Cabicompendium.110287, CABI Compendium. 2022. doi:10.1079/cabicompendium.110287, CABI International.
  16. Kalkhorani NM, Dadgar M, Rezaei MB, Hero Abadi F. Comparison of Essential oil of Ocimum sanctum L. from Fresh and Dry Aerial Parts by Hydro-distillation and Steam Distillation. J Med Plan By-products. 2016; 1: 45-50.
  17. Rajkumar G, Jayasinghe MR, Sanmugarajah V. Comparative Analytical Study of Phytochemicals in Selected Antidiabetic Medicinal Plant Seeds in Sri Lanka, Pharm Sci Res. 2021; 8(3): 145-155. Available at: https://scholarhub.ui.ac.id/psr/vol8/iss3/5
  18. Rajkumar G, Panambara PAHR, Sanmugarajah V. Comparative Analysis of Qualitative and Quantitative Phytochemical Evaluation of Selected Leaves of Medicinal Plants in Jaffna, Sri Lanka. Borneo J Pharm. 2022; 5(2): 93-103. doi: https://doi.org/10.33084/bjop.v5i2.3091
  19. Rajkumar G, Sanmugarajah V, Jayasinghe MR. Comparative Phyto and Physicochemical Parameters of the Therapeutic Plant Syzygium cumini (L.) Skeels in Jaffna District. Current Scientia, Faculty of Applied Sciences, University of Sri Jayewardenepura. 2023; 26 (02): 64-77. https://doi.org/10.31357/vjs.v26i02.6808
  20. Singleton VL, Orthofer R, Raventos RML. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocaltue reagent. Meth Enzymol. 1999; 299: 152-178.
  21. Kavitha Chandran CI, Indira G. Quantitative estimation of total phenolic, flavonoids, tannin and chlorophyll content of leaves of Strobilanthes kunthiana (Neelakurinji). J Med Plan. 2016; 4(6): 282–286.
  22. Badarinath AV, Mallikarjuna Rao K, Chetty CMS, Ramkanth S, Rajan TVS, Gnanaprakash K. A Review on in-vitro antioxidant methods: Comparisons, correlations and considerations. Int J Pharm Tech Res. 2010; 2(2): 1276-1285.
  23. Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol. 2005; 4(7): 685-688.
  24. Aliyu AB, Musa AM, Oshanimi JA, Ibrahim HA, Oyewale AO. Phytochemical analysis and mineral elements composition of some medicinal plants of Northern Nigeria. Niger J Pharm Sci. 2008; 7(1): 119– 125.
  25. Yamaguchi T, Takamura H, Matoba T, Terao J. HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl. Biosci Biotechnol Biochem. 1998; 62(6): 1201-4. doi: 10.1271/bbb.62.1201. PMID: 9692204.
  26. Blois MS. Antioxidant determination by use of stable free radical. Nature. 1958; 181: 1199-1200.
  27. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M. Antioxidant activity applying an improved ABTS radical cation decolorization Assay. Free Radic Biol Med. 999; 26: 1231-37.
  28. Joshi B, Prasad Sah G, Bahadur Basnet B, Raj Bhatt M, Sharma D, Subedi K, Pandey J, Malla R. Phytochemical extraction and antimicrobial properties of different medicinal plants: Ocimum sanctum (Tulsi), Eugenia caryophyllata (Clove), Achyranthes bidentata (Datiwan) and Azadirachta indica (Neem). J Microbiol Antimicrob. 2011; 3(1): 1-7.
  29. Rana Md. M, Sayeed Md. A, Nasrin Mst. S, Islam M, Rahman Md. M, Alam MF. Free radical scavenging potential and phytochemical analysis of leaf extract from Ocimum sanctum Linn. J Agri Tech. 2015;11(7): 1615-1623.
  30. Borah R, Biswas SP. Tulsi (Ocimum sanctum), excellent source of phytochemicals. Int J Environ Agric Biotechnol. 2018; 3 (5).
  31. Xia KZ, Perveen N, Khan NH. Phytochemical analysis, antibacterial and antioxidant activity determination of Ocimum sanctum. Pharma Pharmacol Int J. 2018; 6(6): 490-497. DOI: 10.15406/ppij.2018.06.00223
  32. Harichandan SS, Priyadarshini, Kumar SA, Sakshi G, Rahul N. Phytochemical screening and antioxidant activity of methanolic extract of Ocimum sanctum Linn. Leaves. GSC Biol Pharm Sci. 2019; 08(02): 022-033. DOI: 10.30574/gscbps.2019.8.2.0131
  33. Praveen G, Rajesh G. Phytochemical screening and quantitative estimation of total flavonoids of Ocimum sanctum in different solvent extract. Pharma Innov J. 2019; 8(2): 16-21.
  34. Pathak I, Niraula M. Assessment of Total Phenolic, Flavonoid Content and Antioxidant Activity of Ocimum sanctum Linn. J Nepal Chem Soc. 2019; 40: 30-35.
  35. Chaudhary A, Sharma S, Mittal A, Gupta S, Dua A. Phytochemical and antioxidant profiling of Ocimum sanctum. J Food Sci Technol. 2020; 57(10): 3852-3863. doi: 10.1007/s13197-020-04417-2. Epub 2020 Apr 11. PMID: 32903995; PMCID: PMC7447722.
  36. Roy M, Chandnanai Y, Rooplai B, Roy S. Phytochemical Analysis of Ocimum sanctum L. Leaf Extracts. J Ind Vet Assoc. 2021; 19 (1): 70 – 74.
  37. Nasim Milani Kalkhorani, Mohsen Dadgar, Mohammad Bagher Rezaei and Fatemeh HeroAbadi. Comparison of Essential oil of Ocimum sanctum L. from Fresh and Dry Aerial Parts by Hydro-distillation and Steam Distillation. J Med Plan and By-products. 2016; 1: 45-50.
  38. Rakesh Kumar Joshi. Phytoconstituents, traditional, medicinal and bioactive uses of Tulsi (Ocimum sanctum Linn.): A review. J Pharmacog Phytochem. 2017; 6(2): 261-264.
  39. Kumar R, Saha P, Lokare P, Datta K, Selvakumar P, Chourasia A. A Systemic Review of Ocimum sanctum (Tulsi): Morphological Characteristics, Phytoconstituents and Therapeutic Applications. Int J Res App Sci Biotechnol. 2022; 9(2): 121-126.
  40. Singh J, Neha M, Sinha S. Comparison of Total Flavonoid, Phenolic Content and Antioxidant capacity In Leaf and Seed Extracts from White Holy Basil (Ocimum Sanctum). Int J Appl Biol Pharm Technol. 2014; 5 (4): 34-42.
  41. Singh S, Gupta A, Verma S. Phytochemical Screening, TPC, TFC And Antibacterial Activity Against Uti Pathogen of Ocimum sanctum Plant Leaf Hydro Alcoholic Extract. Palarch’s J Arch Egyptol. 2020; 17(7): 13505 – 13513.
  42. Lin D, Xiao M, Zhao J, Li Z, Xing B, Li X, Kong M, Li L, Zhang Q, Liu Y, Chen H, Qin W, Wu H, Chen S. An Overview of Plant Phenolic Compounds and Their Importance in Human Nutrition and Management of Type 2 Diabetes. Molecules. 2016; 21(10): 1374. doi: 10.3390/molecules21101374. PMID: 27754463; PMCID: PMC6274266.
  43. Luna-Guevara Ma. L, Luna-Guevara JJ, Hernández-Carranza P, Espinosa HR, Ochoa-Velasco CE. Phenolic Compounds: A Good Choice Against Chronic Degenerative Diseases. Chapter 3. Stud Nat Prod Chem. 2018; 59: 79-108. https://doi.org/10.1016/B978-0-444-64179-3.00003-7
  44. Cáceres-Jiménez S, Ordóñez-Díaz JL, Moreno-Rojas JM, Pereira-Caro G. 9 – Bioaccesibility and bioavailability of marine polyphenols. Marine Phenolic Compounds. Sci Eng. 2023: 265-298. https://doi.org/10.1016/B978-0-12-823589-8.00008-X
  45. Gadnayak A, Dehury B. Phytochemicals: recent trends in food, pharmacy, and biotechnology, Chapter 5, Rec Fron Phytochem Appl Food Pharm Cos Biotechnol. 2023: 85-93. https://doi.org/10.1016/B978-0-443-19143-5.00031-1
  46. Pandey KB, Rizvi SI. Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev. 2009; 2(5): 270-8. doi: 10.4161/oxim.2.5.9498. PMID: 20716914; PMCID: PMC2835915.
  47. Hano C, Tungmunnithum D. Plant Polyphenols, more than Just Simple Natural Antioxidants: Oxidative Stress, Aging and Age-Related Diseases. Medicines (Basel). 2020; 9; 7(5): 26. doi: 10.3390/medicines7050026. PMID: 32397520; PMCID: PMC7281114.
  48. Natalia VZ, Zubova MY, Nechaeva TL, Kazantseva VV, Goncharuk EA, Katanskaya VM, Baranova EN, Aksenova MA. Polyphenols in Plants: Structure, Biosynthesis, Abiotic Stress Regulation, and Practical Applications (Review). Int J Mol Sci. 2023; 24 (18): 13874. https://doi.org/10.3390/ijms241813874
  49. Panche AN, Diwan AD, Chandra SR. Flavonoids: an overview. J Nutri Sci. 2016; 5: e47. doi: 10.1017/jns.2016.41. PMID: 28620474; PMCID: PMC5465813.
  50. Passos CP, Domingues MR, Silva CM, Coimbra MA. Book Chapter -Tannins: Types, Foods Containing, and Nutrition, Nova Science Publishers. 2012.
  51. Ashok PK, Upadhyaya K. Tannins are Astringent. J Pharmacog Phytochem. 2012; 1 (3): 45- 50.
  52. Adamczyk B, Simon J, Kitunen V, Adamczyk S, Smolander A. Tannins and Their Complex Interaction with Different Organic Nitrogen Compounds and Enzymes: Old Paradigms versus Recent Advances. Chemistry Open. 2017; 16; 6(5): 610-614. doi: 10.1002/open.201700113. PMID: 29046854; PMCID: PMC5641916.
  53. Amarowicz R. Tannins: the new natural antioxidants? Europ J Lip Sci Technol. Editorial. 2007; 109: 549–551.
  54. Singh AP, Kumar S. Applications of Tannins in Industry. Tannins - Structural Properties, Biological Properties and Current Knowledge. Intech Open. 2020. Available at: http://dx.doi.org/10.5772/intechopen.85984.
  55. Tong Z, He W, Fan X, Guo A. Biological Function of Plant Tannin and Its Application in Animal Health. Front Vet Sci. 2022; 8: 803657. doi: 10.3389/fvets.2021.803657. PMID: 35083309; PMCID: PMC8784788.
  56. Britannica T. Editors of Encyclopaedia. alkaloid. Encyclopedia Britannica. (2025). https://www.britannica.com/science/alkaloid
  57. Sirin S, Dolanbay SN, Aslim B. Role of plant derived alkaloids as antioxidant agents for neurodegenerative diseases, Health Sci Rev. 2023; 6:100071.
  58. Pooja SR. In-Vitro Antioxidant activity of Ocimum sanctum Linn. Int J Tech Res Appl. 2018; 6 (3): 47-54. www.ijtra.com
  59. Nasution H, Wijaya F, Ellsworth. Characterization of antioxidant from daunkemangi (Ocimum sanctum) extracted using ultrasonic bath. J Phy Con Ser. 2020; 1542 012016. DOI 10.1088/1742-6596/1542/1/012016
  60. Saravanan R, Ramamurthy J. Evaluation of Antioxidant Activity of Ocimum Sanctum-An In Vitro Study. Int J Dent Oral Sci. 2021; 8(11): 5001-5005.
  61. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacog Rev. 2010; 4(8): 118-26. doi: 10.4103/0973-7847.70902. PMID: 22228951; PMCID: PMC3249911.
  62. Jyoti S, Satendra S, Sushma S, Anjana T, Shashi S. Antistressor activity of Ocimum sanctum (Tulsi) against experimentally induced oxidative stress in rabbits. Methods Find in Exp Clin Pharmacol. 2007; 29(6): 411-6. doi: 10.1358/mf.2007.29.6.1118135. PMID: 17922070.

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