Antileishmanial Activity of Myrtle Methanolic Extract against Leishmaniamajor: an In Vitro Study

Mahmoudvand, Hossein; Salehi Lalehmarzi, Hamed; Fallahi, Shirzad; Moslehi Baharanchi, Maryam; Bayat, Pardis; Jahanbakhsh, Sareh

doi:10.22087/hmj.v0i0.637

Antileishmanial Activity of Myrtle Methanolic Extract against Leishmaniamajor: an In Vitro Study

Document Type : Original Article

Authors

Hossein Mahmoudvand ¹

Hamed Salehi Lalehmarzi ²

Shirzad Fallahi ¹

Maryam Moslehi Baharanchi ³

Pardis Bayat ³

Sareh Jahanbakhsh ⁴

¹ Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

² Bogomolets National Medical University, Kyiv, Ukraine

³ 3 Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran

⁴ Bam University of Medical Sciences, Bam, Iran

10.22087/hmj.v0i0.637

Abstract

Background and Aim: In this study we assessed the in vitro antileishmanial activity of myrtle (Myrtus communis L.) methanolic extract against Leishmania major.Materials and Methods: The in vitro antileishmanial effects of myrtle methanolic extract against L. major promastigote and amastigotes were determined by colorimetric cell viability (MTT) assay and macrophage model, respectively. The IC50 values were also calculated by probit test in SPSS software.Results:The obtained results showed that myrtle extract was significantly inhibited promastigote growth of L. major based on a dose and time dependent manner. The measured IC50 values for myrtle methanolic extract and MA as control drug against promastigote forms of L. major were 23.6 µg/mL and 88.3 µg/mL, respectively. The obtained IC50 values were 13.8

Keywords

Promastigote

Amastigote

Leishmania major

Medicinal plants

Cutaneous leishmaniasis

Myrtus communis